Bone Marrow Mononuclear Cell Therapy Led to Alveolar-Capillary Membrane Repair, Improving Lung Mechanics in Endotoxin-Induced Acute Lung Injury
The aim of this study was to test the hypothesis that bone marrow mononuclear cell (BMDMC) therapy led an improvement in lung mechanics and histology in endotoxin-induced lung injury. Twenty-four C57BL/6 mice were randomly divided into four groups ( n = 6 each). In the acute lung injur;y (ALI) group...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2010-08-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368910X506845 |
| _version_ | 1818117953165459456 |
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| author | Luiz Felipe M. Prota Roberta M. Lassance Tatiana Maron-Gutierrez Raquel C. Castiglione Cristiane Souza Baez Garcia Maria Cristina Ebole Santana Jackson Souza-Menezes Soraia C. Abreu Vivian Samoto Marcelo Felipe Santiago Vera Luiza Capelozzi Christina Maeda Takiya Patricia R. M. Rocco Marcelo M. Morales |
| author_facet | Luiz Felipe M. Prota Roberta M. Lassance Tatiana Maron-Gutierrez Raquel C. Castiglione Cristiane Souza Baez Garcia Maria Cristina Ebole Santana Jackson Souza-Menezes Soraia C. Abreu Vivian Samoto Marcelo Felipe Santiago Vera Luiza Capelozzi Christina Maeda Takiya Patricia R. M. Rocco Marcelo M. Morales |
| author_sort | Luiz Felipe M. Prota |
| collection | DOAJ |
| description | The aim of this study was to test the hypothesis that bone marrow mononuclear cell (BMDMC) therapy led an improvement in lung mechanics and histology in endotoxin-induced lung injury. Twenty-four C57BL/6 mice were randomly divided into four groups ( n = 6 each). In the acute lung injur;y (ALI) group, Escherichia coli lipopolysaccharide (LPS) was instilled intratracheally (40 μg, IT), and control (C) mice received saline (0.05 ml, IT). One hour after the administration of saline or LPS, BMDMC (2 × 10 7 cells) was intravenously injected. At day 28, animals were anesthetized and lung mechanics [static elastance ( E st ), resistive (Δ P 1 ), and viscoelastic (Δ P 2 ) pressures] and histology (light and electron microscopy) were analyzed. Immunogold electron microscopy was used to evaluate if multinucleate cells were type II epithelial cells. BMDMC therapy prevented endotoxin-induced lung inflammation, alveolar collapse, and interstitial edema. In addition, BMDMC administration led to epithelial and endothelial repair with multinucleated type II pneumocytes. These histological changes yielded a reduction in lung E st , Δ P 1 , and Δ P 2 compared to ALI. In the present experimental ALI model, the administration of BMDMC yielded a reduction in the inflammatory process and a repair of epithelium and endothelium, reducing the amount of alveolar collapse, thus leading to an improvement in lung mechanics. |
| first_indexed | 2024-12-11T04:46:36Z |
| format | Article |
| id | doaj.art-324e7146b0ca4370ab3baf17fea95274 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-11T04:46:36Z |
| publishDate | 2010-08-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-324e7146b0ca4370ab3baf17fea952742022-12-22T01:20:30ZengSAGE PublishingCell Transplantation0963-68971555-38922010-08-011910.3727/096368910X506845Bone Marrow Mononuclear Cell Therapy Led to Alveolar-Capillary Membrane Repair, Improving Lung Mechanics in Endotoxin-Induced Acute Lung InjuryLuiz Felipe M. Prota0Roberta M. Lassance1Tatiana Maron-Gutierrez2Raquel C. Castiglione3Cristiane Souza Baez Garcia4Maria Cristina Ebole Santana5Jackson Souza-Menezes6Soraia C. Abreu7Vivian Samoto8Marcelo Felipe Santiago9Vera Luiza Capelozzi10Christina Maeda Takiya11Patricia R. M. Rocco12Marcelo M. Morales13Laboratory of Cellular and Molecular Physiology, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilLaboratory of Cellular and Molecular Physiology, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilLaboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilLaboratory of Cellular and Molecular Physiology, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilLaboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilLaboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilFederal University of Rio de Janeiro, Macaé, BrazilLaboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilLaboratory of Cell Pathology, Biomedical Science Institute, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilLaboratory of Cellular and Molecular Neurology, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilDepartment of Pathology, University of São Paulo, São Paulo, BrazilLaboratory of Cell Pathology, Biomedical Science Institute, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilLaboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilLaboratory of Cellular and Molecular Physiology, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilThe aim of this study was to test the hypothesis that bone marrow mononuclear cell (BMDMC) therapy led an improvement in lung mechanics and histology in endotoxin-induced lung injury. Twenty-four C57BL/6 mice were randomly divided into four groups ( n = 6 each). In the acute lung injur;y (ALI) group, Escherichia coli lipopolysaccharide (LPS) was instilled intratracheally (40 μg, IT), and control (C) mice received saline (0.05 ml, IT). One hour after the administration of saline or LPS, BMDMC (2 × 10 7 cells) was intravenously injected. At day 28, animals were anesthetized and lung mechanics [static elastance ( E st ), resistive (Δ P 1 ), and viscoelastic (Δ P 2 ) pressures] and histology (light and electron microscopy) were analyzed. Immunogold electron microscopy was used to evaluate if multinucleate cells were type II epithelial cells. BMDMC therapy prevented endotoxin-induced lung inflammation, alveolar collapse, and interstitial edema. In addition, BMDMC administration led to epithelial and endothelial repair with multinucleated type II pneumocytes. These histological changes yielded a reduction in lung E st , Δ P 1 , and Δ P 2 compared to ALI. In the present experimental ALI model, the administration of BMDMC yielded a reduction in the inflammatory process and a repair of epithelium and endothelium, reducing the amount of alveolar collapse, thus leading to an improvement in lung mechanics.https://doi.org/10.3727/096368910X506845 |
| spellingShingle | Luiz Felipe M. Prota Roberta M. Lassance Tatiana Maron-Gutierrez Raquel C. Castiglione Cristiane Souza Baez Garcia Maria Cristina Ebole Santana Jackson Souza-Menezes Soraia C. Abreu Vivian Samoto Marcelo Felipe Santiago Vera Luiza Capelozzi Christina Maeda Takiya Patricia R. M. Rocco Marcelo M. Morales Bone Marrow Mononuclear Cell Therapy Led to Alveolar-Capillary Membrane Repair, Improving Lung Mechanics in Endotoxin-Induced Acute Lung Injury Cell Transplantation |
| title | Bone Marrow Mononuclear Cell Therapy Led to Alveolar-Capillary Membrane Repair, Improving Lung Mechanics in Endotoxin-Induced Acute Lung Injury |
| title_full | Bone Marrow Mononuclear Cell Therapy Led to Alveolar-Capillary Membrane Repair, Improving Lung Mechanics in Endotoxin-Induced Acute Lung Injury |
| title_fullStr | Bone Marrow Mononuclear Cell Therapy Led to Alveolar-Capillary Membrane Repair, Improving Lung Mechanics in Endotoxin-Induced Acute Lung Injury |
| title_full_unstemmed | Bone Marrow Mononuclear Cell Therapy Led to Alveolar-Capillary Membrane Repair, Improving Lung Mechanics in Endotoxin-Induced Acute Lung Injury |
| title_short | Bone Marrow Mononuclear Cell Therapy Led to Alveolar-Capillary Membrane Repair, Improving Lung Mechanics in Endotoxin-Induced Acute Lung Injury |
| title_sort | bone marrow mononuclear cell therapy led to alveolar capillary membrane repair improving lung mechanics in endotoxin induced acute lung injury |
| url | https://doi.org/10.3727/096368910X506845 |
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