Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds
Abstract: Quick tests are essential tools to evaluate seed quality. The objective of this study was to adapt the methodology of tetrazolium test, by identifying the most suitable pre-conditioning parameters of imbibition temperature and duration, and tetrazolium solution concentrations, in order to...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Tecnologia de Sementes
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| Series: | Journal of Seed Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372019000100007&lng=en&tlng=en |
| _version_ | 1818300069830459392 |
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| author | Humberto Alencar Paraíso Delacyr da Silva Brandão Junior Ramon Ivo Soares Avelar Candido Alves da Costa Luan Souza de Paula Gomes Warley Marcos Nascimento |
| author_facet | Humberto Alencar Paraíso Delacyr da Silva Brandão Junior Ramon Ivo Soares Avelar Candido Alves da Costa Luan Souza de Paula Gomes Warley Marcos Nascimento |
| author_sort | Humberto Alencar Paraíso |
| collection | DOAJ |
| description | Abstract: Quick tests are essential tools to evaluate seed quality. The objective of this study was to adapt the methodology of tetrazolium test, by identifying the most suitable pre-conditioning parameters of imbibition temperature and duration, and tetrazolium solution concentrations, in order to assess the physiological quality of chickpea seeds. Also, this work proposed the separation of lots in classes, according to the viability and vigor of the seeds. Three lots of chickpeas (lot 1 - BRS Aleppo C1, lot 2 - BRS Aleppo basic, and lot 3 - Cicero) were evaluated. They were analyzed according to three combinations of imbibition temperature and duration (41 °C for 4 hours, 41 ºC for 6 hours, and 30 ºC for 18 hours) and two tetrazolium concentrations (0.1% and 0.5%). The imbibition at 41 °C for 4 or 6 hours, and at 30 ºC for 18 hours allowed clear visualization of injuries in the seeds after immersion in 0.1% tetrazolium solution. The intense coloration formed by the 0.5% solution of this salt prevented the differentiation of the types of damage. In addition, it was possible to separate the seeds into four classes: class I (viable and vigorous, without damages), class II (viable and vigorous, with superficial injuries), class III (viable and non-vigorous), and class IV (non-viable). |
| first_indexed | 2024-12-13T05:01:16Z |
| format | Article |
| id | doaj.art-324ffafbe94a41d9b21b9498e28b9349 |
| institution | Directory Open Access Journal |
| issn | 2317-1545 |
| language | English |
| last_indexed | 2024-12-13T05:01:16Z |
| publisher | Associação Brasileira de Tecnologia de Sementes |
| record_format | Article |
| series | Journal of Seed Science |
| spelling | doaj.art-324ffafbe94a41d9b21b9498e28b93492022-12-21T23:58:46ZengAssociação Brasileira de Tecnologia de SementesJournal of Seed Science2317-154541171210.1590/2317-1545v41n1187777S2317-15372019000100007Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seedsHumberto Alencar ParaísoDelacyr da Silva Brandão JuniorRamon Ivo Soares AvelarCandido Alves da CostaLuan Souza de Paula GomesWarley Marcos NascimentoAbstract: Quick tests are essential tools to evaluate seed quality. The objective of this study was to adapt the methodology of tetrazolium test, by identifying the most suitable pre-conditioning parameters of imbibition temperature and duration, and tetrazolium solution concentrations, in order to assess the physiological quality of chickpea seeds. Also, this work proposed the separation of lots in classes, according to the viability and vigor of the seeds. Three lots of chickpeas (lot 1 - BRS Aleppo C1, lot 2 - BRS Aleppo basic, and lot 3 - Cicero) were evaluated. They were analyzed according to three combinations of imbibition temperature and duration (41 °C for 4 hours, 41 ºC for 6 hours, and 30 ºC for 18 hours) and two tetrazolium concentrations (0.1% and 0.5%). The imbibition at 41 °C for 4 or 6 hours, and at 30 ºC for 18 hours allowed clear visualization of injuries in the seeds after immersion in 0.1% tetrazolium solution. The intense coloration formed by the 0.5% solution of this salt prevented the differentiation of the types of damage. In addition, it was possible to separate the seeds into four classes: class I (viable and vigorous, without damages), class II (viable and vigorous, with superficial injuries), class III (viable and non-vigorous), and class IV (non-viable).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372019000100007&lng=en&tlng=enCicer arietinumseed analysisviabilityvigor |
| spellingShingle | Humberto Alencar Paraíso Delacyr da Silva Brandão Junior Ramon Ivo Soares Avelar Candido Alves da Costa Luan Souza de Paula Gomes Warley Marcos Nascimento Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds Journal of Seed Science Cicer arietinum seed analysis viability vigor |
| title | Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds |
| title_full | Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds |
| title_fullStr | Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds |
| title_full_unstemmed | Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds |
| title_short | Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds |
| title_sort | adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds |
| topic | Cicer arietinum seed analysis viability vigor |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372019000100007&lng=en&tlng=en |
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