Overexpressed p-S6 associates with lymph node metastasis and predicts poor prognosis in non-small cell lung cancer
Abstract Background Ribosomal protein S6 (S6), a downstream effect media of the AKT/mTOR pathway, not only is a part of 40S small subunit of eukaryotic ribosome, but also involves in protein synthesis and cell proliferation during cancer development. Methods In present study, we explore the associat...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-05-01
|
| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-022-09664-4 |
| _version_ | 1818549699583410176 |
|---|---|
| author | Yaoxiang Tang Jiadi Luo Ying Zhou Hongjing Zang Yang Yang Sile Liu Hongmei Zheng Jian Ma Songqing Fan Qiuyuan Wen |
| author_facet | Yaoxiang Tang Jiadi Luo Ying Zhou Hongjing Zang Yang Yang Sile Liu Hongmei Zheng Jian Ma Songqing Fan Qiuyuan Wen |
| author_sort | Yaoxiang Tang |
| collection | DOAJ |
| description | Abstract Background Ribosomal protein S6 (S6), a downstream effect media of the AKT/mTOR pathway, not only is a part of 40S small subunit of eukaryotic ribosome, but also involves in protein synthesis and cell proliferation during cancer development. Methods In present study, we explore the association between phosphorylated S6 (p-S6) protein expression and clinicopathological features as well as prognostic implications in NSCLC. P-S6 was detected in tissue microarrays (TMAs) containing 350 NSCLC, 53 non-cancerous lung tissues (Non-CLT), and 88 cases of matched metastatic lymph node lesions via immunohistochemistry (IHC). Transwell assays and wound healing assay were used to assess the effects of p-S6 inhibition on NSCLC cell metastasis. Results The p-S6 expression in NSCLC was more evident than that in Non-CLT (p < 0.05). Compared to NSCLC patients who have no lymph node metastasis (LNM), those with LNM had higher p-S6 expression (p = 0.001). Regardless of lung squamous cell carcinoma (SCC) or adenocarcinoma (ADC), p-S6 was increased obviously in metastatic lymph nodes compared with matched primary cancers (p = 0.001, p = 0.022, respectively). Inhibition of p-S6 decreased the metastasis ability of NSCLC cells. In addition, p-S6 was an independent predicted marker for LNM in patients with NSCLC (p < 0.001). According to survival analysis, patients with highly expressed p-S6 had a lower survival rate compared with that with lower expression (p = 0.013). P-S6 is an unfavorable independent prognostic factor for NSCLC patients (p = 0.011). Conclusion Increased expression of p-S6 is not only a novel predictive biomarker of LNM but also poor prognosis in NSCLC. |
| first_indexed | 2024-12-12T08:36:45Z |
| format | Article |
| id | doaj.art-3255522741b9492c822e0d08b5d1a650 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-12T08:36:45Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-3255522741b9492c822e0d08b5d1a6502022-12-22T00:30:54ZengBMCBMC Cancer1471-24072022-05-0122111110.1186/s12885-022-09664-4Overexpressed p-S6 associates with lymph node metastasis and predicts poor prognosis in non-small cell lung cancerYaoxiang Tang0Jiadi Luo1Ying Zhou2Hongjing Zang3Yang Yang4Sile Liu5Hongmei Zheng6Jian Ma7Songqing Fan8Qiuyuan Wen9Department of Pathology, The Second Xiangya Hospital, Central South UniversityDepartment of Pathology, The Second Xiangya Hospital, Central South UniversityDepartment of Pathology, The Second Xiangya Hospital, Central South UniversityDepartment of Pathology, The Second Xiangya Hospital, Central South UniversityDepartment of Pathology, The Second Xiangya Hospital, Central South UniversityDepartment of Pathology, The Second Xiangya Hospital, Central South UniversityDepartment of Pathology, The Second Xiangya Hospital, Central South UniversityCancer Research Institute of Central South University, Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Central South UniversityDepartment of Pathology, The Second Xiangya Hospital, Central South UniversityDepartment of Pathology, The Second Xiangya Hospital, Central South UniversityAbstract Background Ribosomal protein S6 (S6), a downstream effect media of the AKT/mTOR pathway, not only is a part of 40S small subunit of eukaryotic ribosome, but also involves in protein synthesis and cell proliferation during cancer development. Methods In present study, we explore the association between phosphorylated S6 (p-S6) protein expression and clinicopathological features as well as prognostic implications in NSCLC. P-S6 was detected in tissue microarrays (TMAs) containing 350 NSCLC, 53 non-cancerous lung tissues (Non-CLT), and 88 cases of matched metastatic lymph node lesions via immunohistochemistry (IHC). Transwell assays and wound healing assay were used to assess the effects of p-S6 inhibition on NSCLC cell metastasis. Results The p-S6 expression in NSCLC was more evident than that in Non-CLT (p < 0.05). Compared to NSCLC patients who have no lymph node metastasis (LNM), those with LNM had higher p-S6 expression (p = 0.001). Regardless of lung squamous cell carcinoma (SCC) or adenocarcinoma (ADC), p-S6 was increased obviously in metastatic lymph nodes compared with matched primary cancers (p = 0.001, p = 0.022, respectively). Inhibition of p-S6 decreased the metastasis ability of NSCLC cells. In addition, p-S6 was an independent predicted marker for LNM in patients with NSCLC (p < 0.001). According to survival analysis, patients with highly expressed p-S6 had a lower survival rate compared with that with lower expression (p = 0.013). P-S6 is an unfavorable independent prognostic factor for NSCLC patients (p = 0.011). Conclusion Increased expression of p-S6 is not only a novel predictive biomarker of LNM but also poor prognosis in NSCLC.https://doi.org/10.1186/s12885-022-09664-4P-S6Lymph node metastasisPrognosisNon-small cell lung cancer |
| spellingShingle | Yaoxiang Tang Jiadi Luo Ying Zhou Hongjing Zang Yang Yang Sile Liu Hongmei Zheng Jian Ma Songqing Fan Qiuyuan Wen Overexpressed p-S6 associates with lymph node metastasis and predicts poor prognosis in non-small cell lung cancer BMC Cancer P-S6 Lymph node metastasis Prognosis Non-small cell lung cancer |
| title | Overexpressed p-S6 associates with lymph node metastasis and predicts poor prognosis in non-small cell lung cancer |
| title_full | Overexpressed p-S6 associates with lymph node metastasis and predicts poor prognosis in non-small cell lung cancer |
| title_fullStr | Overexpressed p-S6 associates with lymph node metastasis and predicts poor prognosis in non-small cell lung cancer |
| title_full_unstemmed | Overexpressed p-S6 associates with lymph node metastasis and predicts poor prognosis in non-small cell lung cancer |
| title_short | Overexpressed p-S6 associates with lymph node metastasis and predicts poor prognosis in non-small cell lung cancer |
| title_sort | overexpressed p s6 associates with lymph node metastasis and predicts poor prognosis in non small cell lung cancer |
| topic | P-S6 Lymph node metastasis Prognosis Non-small cell lung cancer |
| url | https://doi.org/10.1186/s12885-022-09664-4 |
| work_keys_str_mv | AT yaoxiangtang overexpressedps6associateswithlymphnodemetastasisandpredictspoorprognosisinnonsmallcelllungcancer AT jiadiluo overexpressedps6associateswithlymphnodemetastasisandpredictspoorprognosisinnonsmallcelllungcancer AT yingzhou overexpressedps6associateswithlymphnodemetastasisandpredictspoorprognosisinnonsmallcelllungcancer AT hongjingzang overexpressedps6associateswithlymphnodemetastasisandpredictspoorprognosisinnonsmallcelllungcancer AT yangyang overexpressedps6associateswithlymphnodemetastasisandpredictspoorprognosisinnonsmallcelllungcancer AT sileliu overexpressedps6associateswithlymphnodemetastasisandpredictspoorprognosisinnonsmallcelllungcancer AT hongmeizheng overexpressedps6associateswithlymphnodemetastasisandpredictspoorprognosisinnonsmallcelllungcancer AT jianma overexpressedps6associateswithlymphnodemetastasisandpredictspoorprognosisinnonsmallcelllungcancer AT songqingfan overexpressedps6associateswithlymphnodemetastasisandpredictspoorprognosisinnonsmallcelllungcancer AT qiuyuanwen overexpressedps6associateswithlymphnodemetastasisandpredictspoorprognosisinnonsmallcelllungcancer |