Comparative Transcriptome Analysis of <i>Eriocheir sinensis</i> from Wild Habitats in Han River, Korea
<i>Eriocheir sinensis</i> is an euryhaline crab found from East Asia to Europe and North America. This species can live in freshwater and seawater due to the unique physiological characteristics of their life cycle, which allows them to adapt and inhabit different habitats in a wide rang...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/12/12/2027 |
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| author | Hyung-Eun An Tae-June Choi Chang-Bae Kim |
| author_facet | Hyung-Eun An Tae-June Choi Chang-Bae Kim |
| author_sort | Hyung-Eun An |
| collection | DOAJ |
| description | <i>Eriocheir sinensis</i> is an euryhaline crab found from East Asia to Europe and North America. This species can live in freshwater and seawater due to the unique physiological characteristics of their life cycle, which allows them to adapt and inhabit different habitats in a wide range of environments. Despite the wealth of studies focusing on adaptation mechanism of <i>E. sinensis</i> to specific environmental factors, the adaptation mechanisms to wild habitats with coexisting environmental factors are not well understood. In this study, we conducted a transcriptome analysis to investigate gene expression differences related to habitat adaptation of <i>E. sinensis</i> from two wild habitats with different environmental factors in the Han River, Korea. A total of 138,261 unigenes were analyzed, of which 228 were analyzed as differentially expressed genes (DEGs) between the two wild habitats. Among 228 DEGs, 110 DEGs were annotated against databases; most DEGs were involved in energy metabolism, immunity, and osmoregulation. Moreover, DEG enrichment analysis showed that upregulated genes were related to biosynthesis, metabolism, and immunity in an habitat representing relatively high salinity whereas downregulated genes were related to ion transport and hypoxia response in habitats with relatively low salinity and dissolved oxygen. The present findings can serve as foundation for future <i>E. sinensis</i> culture or conservation approaches in natural conditions. |
| first_indexed | 2024-03-09T16:10:44Z |
| format | Article |
| id | doaj.art-325599ff68554f7ca3baa8e847da3a9d |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T16:10:44Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-325599ff68554f7ca3baa8e847da3a9d2023-11-24T16:12:21ZengMDPI AGLife2075-17292022-12-011212202710.3390/life12122027Comparative Transcriptome Analysis of <i>Eriocheir sinensis</i> from Wild Habitats in Han River, KoreaHyung-Eun An0Tae-June Choi1Chang-Bae Kim2Department of Biotechnology, Sangmyung University, Seoul 03016, Republic of KoreaDepartment of Biotechnology, Sangmyung University, Seoul 03016, Republic of KoreaDepartment of Biotechnology, Sangmyung University, Seoul 03016, Republic of Korea<i>Eriocheir sinensis</i> is an euryhaline crab found from East Asia to Europe and North America. This species can live in freshwater and seawater due to the unique physiological characteristics of their life cycle, which allows them to adapt and inhabit different habitats in a wide range of environments. Despite the wealth of studies focusing on adaptation mechanism of <i>E. sinensis</i> to specific environmental factors, the adaptation mechanisms to wild habitats with coexisting environmental factors are not well understood. In this study, we conducted a transcriptome analysis to investigate gene expression differences related to habitat adaptation of <i>E. sinensis</i> from two wild habitats with different environmental factors in the Han River, Korea. A total of 138,261 unigenes were analyzed, of which 228 were analyzed as differentially expressed genes (DEGs) between the two wild habitats. Among 228 DEGs, 110 DEGs were annotated against databases; most DEGs were involved in energy metabolism, immunity, and osmoregulation. Moreover, DEG enrichment analysis showed that upregulated genes were related to biosynthesis, metabolism, and immunity in an habitat representing relatively high salinity whereas downregulated genes were related to ion transport and hypoxia response in habitats with relatively low salinity and dissolved oxygen. The present findings can serve as foundation for future <i>E. sinensis</i> culture or conservation approaches in natural conditions.https://www.mdpi.com/2075-1729/12/12/2027<i>Eriocheir sinensis</i>transcriptomeRNA-Seqdifferential expressed geneswild habitat |
| spellingShingle | Hyung-Eun An Tae-June Choi Chang-Bae Kim Comparative Transcriptome Analysis of <i>Eriocheir sinensis</i> from Wild Habitats in Han River, Korea Life <i>Eriocheir sinensis</i> transcriptome RNA-Seq differential expressed genes wild habitat |
| title | Comparative Transcriptome Analysis of <i>Eriocheir sinensis</i> from Wild Habitats in Han River, Korea |
| title_full | Comparative Transcriptome Analysis of <i>Eriocheir sinensis</i> from Wild Habitats in Han River, Korea |
| title_fullStr | Comparative Transcriptome Analysis of <i>Eriocheir sinensis</i> from Wild Habitats in Han River, Korea |
| title_full_unstemmed | Comparative Transcriptome Analysis of <i>Eriocheir sinensis</i> from Wild Habitats in Han River, Korea |
| title_short | Comparative Transcriptome Analysis of <i>Eriocheir sinensis</i> from Wild Habitats in Han River, Korea |
| title_sort | comparative transcriptome analysis of i eriocheir sinensis i from wild habitats in han river korea |
| topic | <i>Eriocheir sinensis</i> transcriptome RNA-Seq differential expressed genes wild habitat |
| url | https://www.mdpi.com/2075-1729/12/12/2027 |
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