Integrative analysis of ferroptosis-related genes in ulcerative colitis
Objective The aim of this study was to identify and validate ferroptosis-related markers in ulcerative colitis (UC) to explore new directions for UC diagnosis and treatment. Methods We screened UC chips and ferroptosis-related genes from the Gene Expression Omnibus (GEO), FerrDb, and GeneCards datab...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2021-09-01
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| Series: | Journal of International Medical Research |
| Online Access: | https://doi.org/10.1177/03000605211042975 |
| _version_ | 1818589114304299008 |
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| author | De-jun Cui Chen Chen Wen-qiang Yuan Yun-han Yang Lu Han |
| author_facet | De-jun Cui Chen Chen Wen-qiang Yuan Yun-han Yang Lu Han |
| author_sort | De-jun Cui |
| collection | DOAJ |
| description | Objective The aim of this study was to identify and validate ferroptosis-related markers in ulcerative colitis (UC) to explore new directions for UC diagnosis and treatment. Methods We screened UC chips and ferroptosis-related genes from the Gene Expression Omnibus (GEO), FerrDb, and GeneCards databases. The differentially expressed genes (DEGs) and ferroptosis-related DEGs between the UC group and normal controls were analyzed using bioinformatics methods. Enrichment analysis, protein–protein interaction analysis, and hub genes were screened. Peripheral blood chip and animal experiments were used to validate the ferroptosis-related hub genes. Finally, hub gene–transcription factor, hub gene–microRNA (miRNA), and hub gene–drug interaction networks were constructed. Results Overall, 26 ferroptosis-related DEGs were identified that were significantly enriched in energy pathways and metabolism. We identified ten ferroptosis-related hub genes from the protein–protein interaction network: IL6 , PTGS2 , HIF1A , CD44 , MUC1 , CAV1 , NOS2 , CXCL2 , SCD , and ACSL4 . In the peripheral blood chip GSE94648, CD44 and MUC1 were upregulated, which was consistent with the expression trend in GSE75214. Animal experiments showed that CD44 expression was significantly increased in the colon. Conclusions Our findings indicate that CD44 and MUC1 may be ferroptosis-related markers in UC. |
| first_indexed | 2024-12-16T09:35:30Z |
| format | Article |
| id | doaj.art-32574de3326d4130ae620f0d262e1069 |
| institution | Directory Open Access Journal |
| issn | 1473-2300 |
| language | English |
| last_indexed | 2024-12-16T09:35:30Z |
| publishDate | 2021-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of International Medical Research |
| spelling | doaj.art-32574de3326d4130ae620f0d262e10692022-12-21T22:36:25ZengSAGE PublishingJournal of International Medical Research1473-23002021-09-014910.1177/03000605211042975Integrative analysis of ferroptosis-related genes in ulcerative colitisDe-jun CuiChen ChenWen-qiang YuanYun-han YangLu HanObjective The aim of this study was to identify and validate ferroptosis-related markers in ulcerative colitis (UC) to explore new directions for UC diagnosis and treatment. Methods We screened UC chips and ferroptosis-related genes from the Gene Expression Omnibus (GEO), FerrDb, and GeneCards databases. The differentially expressed genes (DEGs) and ferroptosis-related DEGs between the UC group and normal controls were analyzed using bioinformatics methods. Enrichment analysis, protein–protein interaction analysis, and hub genes were screened. Peripheral blood chip and animal experiments were used to validate the ferroptosis-related hub genes. Finally, hub gene–transcription factor, hub gene–microRNA (miRNA), and hub gene–drug interaction networks were constructed. Results Overall, 26 ferroptosis-related DEGs were identified that were significantly enriched in energy pathways and metabolism. We identified ten ferroptosis-related hub genes from the protein–protein interaction network: IL6 , PTGS2 , HIF1A , CD44 , MUC1 , CAV1 , NOS2 , CXCL2 , SCD , and ACSL4 . In the peripheral blood chip GSE94648, CD44 and MUC1 were upregulated, which was consistent with the expression trend in GSE75214. Animal experiments showed that CD44 expression was significantly increased in the colon. Conclusions Our findings indicate that CD44 and MUC1 may be ferroptosis-related markers in UC.https://doi.org/10.1177/03000605211042975 |
| spellingShingle | De-jun Cui Chen Chen Wen-qiang Yuan Yun-han Yang Lu Han Integrative analysis of ferroptosis-related genes in ulcerative colitis Journal of International Medical Research |
| title | Integrative analysis of ferroptosis-related genes in ulcerative colitis |
| title_full | Integrative analysis of ferroptosis-related genes in ulcerative colitis |
| title_fullStr | Integrative analysis of ferroptosis-related genes in ulcerative colitis |
| title_full_unstemmed | Integrative analysis of ferroptosis-related genes in ulcerative colitis |
| title_short | Integrative analysis of ferroptosis-related genes in ulcerative colitis |
| title_sort | integrative analysis of ferroptosis related genes in ulcerative colitis |
| url | https://doi.org/10.1177/03000605211042975 |
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