Resveratrol modulates cytokine-induced Jak/STAT activation more efficiently than 5-aminosalicylic acid: an in vitro approach.
Many advances have been recently made focused on the valuable help of dietary polyphenols in chronic inflammatory diseases. On the other hand, current treatment options for intestinal bowel disease patients are unsatisfying and, for this reason, it is estimated that many patients use dietary supplem...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4182878?pdf=render |
| _version_ | 1811322529895153664 |
|---|---|
| author | Diana Serra Ana T Rufino Alexandrina F Mendes Leonor M Almeida Teresa C P Dinis |
| author_facet | Diana Serra Ana T Rufino Alexandrina F Mendes Leonor M Almeida Teresa C P Dinis |
| author_sort | Diana Serra |
| collection | DOAJ |
| description | Many advances have been recently made focused on the valuable help of dietary polyphenols in chronic inflammatory diseases. On the other hand, current treatment options for intestinal bowel disease patients are unsatisfying and, for this reason, it is estimated that many patients use dietary supplements to achieve extra benefits.The aim of this work was to analyze under a mechanistic perspective the anti-inflammatory potential of resveratrol, a natural polyphenolic compound, and to compare it with a pharmaceutical agent, 5-aminosalicylic acid, using the intestinal HT-29 cell line, as a cellular model.In the present study, HT-29 colon epithelial cells were pre-treated with 25 µM resveratrol and/or 500 µM 5-aminosalicylic acid and then exposed to a combination of cytokines (IL-1α, TNF-α, IFN-γ) for a certain period of time. Our data showed that resveratrol, used in a concentration 20 times lower than 5-aminosalicylic acid, was able to significantly reduce NO and PGE2 production, iNOS and COX-2 expression and reactive oxidant species formation induced by the cytokine challenge. However, as already verified with 5-aminosalicylic acid, in spite of not exhibiting any effect on IkB-α degradation, resveratrol down-regulated JAK-STAT pathway, decreasing the levels of activated STAT1 in the nucleus. Additionally, resveratrol decreased the cytokine-stimulated activation of SAPK/JNK pathway but did not counteract the cytokine-triggered negative feedback mechanism of STAT1, through p38 MAPK.Taken together, our results show that resveratrol may be considered a future nutraceutical approach, promoting remission periods, limiting the inflammatory process and preventing colorectal cancer, which is common in these patients. |
| first_indexed | 2024-04-13T13:37:45Z |
| format | Article |
| id | doaj.art-325ac200c35647b98e88b6b07a9f349e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T13:37:45Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-325ac200c35647b98e88b6b07a9f349e2022-12-22T02:44:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e10904810.1371/journal.pone.0109048Resveratrol modulates cytokine-induced Jak/STAT activation more efficiently than 5-aminosalicylic acid: an in vitro approach.Diana SerraAna T RufinoAlexandrina F MendesLeonor M AlmeidaTeresa C P DinisMany advances have been recently made focused on the valuable help of dietary polyphenols in chronic inflammatory diseases. On the other hand, current treatment options for intestinal bowel disease patients are unsatisfying and, for this reason, it is estimated that many patients use dietary supplements to achieve extra benefits.The aim of this work was to analyze under a mechanistic perspective the anti-inflammatory potential of resveratrol, a natural polyphenolic compound, and to compare it with a pharmaceutical agent, 5-aminosalicylic acid, using the intestinal HT-29 cell line, as a cellular model.In the present study, HT-29 colon epithelial cells were pre-treated with 25 µM resveratrol and/or 500 µM 5-aminosalicylic acid and then exposed to a combination of cytokines (IL-1α, TNF-α, IFN-γ) for a certain period of time. Our data showed that resveratrol, used in a concentration 20 times lower than 5-aminosalicylic acid, was able to significantly reduce NO and PGE2 production, iNOS and COX-2 expression and reactive oxidant species formation induced by the cytokine challenge. However, as already verified with 5-aminosalicylic acid, in spite of not exhibiting any effect on IkB-α degradation, resveratrol down-regulated JAK-STAT pathway, decreasing the levels of activated STAT1 in the nucleus. Additionally, resveratrol decreased the cytokine-stimulated activation of SAPK/JNK pathway but did not counteract the cytokine-triggered negative feedback mechanism of STAT1, through p38 MAPK.Taken together, our results show that resveratrol may be considered a future nutraceutical approach, promoting remission periods, limiting the inflammatory process and preventing colorectal cancer, which is common in these patients.http://europepmc.org/articles/PMC4182878?pdf=render |
| spellingShingle | Diana Serra Ana T Rufino Alexandrina F Mendes Leonor M Almeida Teresa C P Dinis Resveratrol modulates cytokine-induced Jak/STAT activation more efficiently than 5-aminosalicylic acid: an in vitro approach. PLoS ONE |
| title | Resveratrol modulates cytokine-induced Jak/STAT activation more efficiently than 5-aminosalicylic acid: an in vitro approach. |
| title_full | Resveratrol modulates cytokine-induced Jak/STAT activation more efficiently than 5-aminosalicylic acid: an in vitro approach. |
| title_fullStr | Resveratrol modulates cytokine-induced Jak/STAT activation more efficiently than 5-aminosalicylic acid: an in vitro approach. |
| title_full_unstemmed | Resveratrol modulates cytokine-induced Jak/STAT activation more efficiently than 5-aminosalicylic acid: an in vitro approach. |
| title_short | Resveratrol modulates cytokine-induced Jak/STAT activation more efficiently than 5-aminosalicylic acid: an in vitro approach. |
| title_sort | resveratrol modulates cytokine induced jak stat activation more efficiently than 5 aminosalicylic acid an in vitro approach |
| url | http://europepmc.org/articles/PMC4182878?pdf=render |
| work_keys_str_mv | AT dianaserra resveratrolmodulatescytokineinducedjakstatactivationmoreefficientlythan5aminosalicylicacidaninvitroapproach AT anatrufino resveratrolmodulatescytokineinducedjakstatactivationmoreefficientlythan5aminosalicylicacidaninvitroapproach AT alexandrinafmendes resveratrolmodulatescytokineinducedjakstatactivationmoreefficientlythan5aminosalicylicacidaninvitroapproach AT leonormalmeida resveratrolmodulatescytokineinducedjakstatactivationmoreefficientlythan5aminosalicylicacidaninvitroapproach AT teresacpdinis resveratrolmodulatescytokineinducedjakstatactivationmoreefficientlythan5aminosalicylicacidaninvitroapproach |