Investigating the therapeutic potential of Bifidobacterium breve and Lactobacillus rhamnosus postbiotics through apoptosis induction in colorectal HT-29 cancer cells
Background and Objectives: Colorectal cancer (CRC) is a prevalent form of cancer worldwide. Recent studies suggest that postbiotics derived from probiotic bacteria have the potential as an adjunct therapy for CRC. This study investigates the anti-cancer effects of Bifidobacterium breve (B. breve) a...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2024-02-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4340 |
| _version_ | 1797303918459355136 |
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| author | Nafiseh Erfanian Hossein Safarpour Tahmineh Tavakoli Mohammad Ali Mahdiabadi Saeed Nasseri Mohammad Hassan Namaei |
| author_facet | Nafiseh Erfanian Hossein Safarpour Tahmineh Tavakoli Mohammad Ali Mahdiabadi Saeed Nasseri Mohammad Hassan Namaei |
| author_sort | Nafiseh Erfanian |
| collection | DOAJ |
| description |
Background and Objectives: Colorectal cancer (CRC) is a prevalent form of cancer worldwide. Recent studies suggest that postbiotics derived from probiotic bacteria have the potential as an adjunct therapy for CRC. This study investigates the anti-cancer effects of Bifidobacterium breve (B. breve) and Lactobacillus rhamnosus (L. rhamnosus) postbiotics on the HT-29 cell line.
Materials and Methods: Through MTT and scratch assay, we investigated the anti-proliferation and anti-migration effects of B. breve and L. rhamnosus postbiotics on HT-29 cells. Furthermore, postbiotic-mediated apoptosis was assessed by analyzing the expression of Bax, Bcl-2, and caspase-3. We also investigated the effects of B. breve postbiotics on the expression of three important genes involved in metastasis, including RSPO2, NGF, and MMP7. Consequently, we validated the expression of selected genes in twelve adenocarcinoma tissues.
Results: The results demonstrated the significant impact of postbiotics on HT-29 cells, highlighting their ability to induce anti-proliferation, anti-migration, and apoptosis-related effects. Notably, these effects were more pronounced using B. breve postbiotics than L. rhamnosus. Additionally, B. breve postbiotics could inhibit metastasis through upregulation of RSPO2 while downregulating NGF and MMP7 expression in HT-29 cells.
Conclusion: Our research suggests that postbiotic metabolites may be effective biological products for the prevention and treatment of cancer.
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| first_indexed | 2024-03-08T00:01:40Z |
| format | Article |
| id | doaj.art-325ade4e9c624aeb8980b895476a7c12 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-03-08T00:01:40Z |
| publishDate | 2024-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-325ade4e9c624aeb8980b895476a7c122024-02-18T04:04:18ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472024-02-0116110.18502/ijm.v16i1.14873Investigating the therapeutic potential of Bifidobacterium breve and Lactobacillus rhamnosus postbiotics through apoptosis induction in colorectal HT-29 cancer cellsNafiseh Erfanian0Hossein Safarpour1Tahmineh Tavakoli2Mohammad Ali Mahdiabadi3Saeed Nasseri4Mohammad Hassan Namaei5Student Research Committee, Birjand University of Medical Sciences, Birjand, IranCellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, IranDepartment of Internal Medicine, School of Medicine, Birjand University of Medical Sciences, Birjand, IranDepartment of Internal Medicine, School of Medicine, Birjand University of Medical Sciences, Birjand, IranCellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, IranInfectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran Background and Objectives: Colorectal cancer (CRC) is a prevalent form of cancer worldwide. Recent studies suggest that postbiotics derived from probiotic bacteria have the potential as an adjunct therapy for CRC. This study investigates the anti-cancer effects of Bifidobacterium breve (B. breve) and Lactobacillus rhamnosus (L. rhamnosus) postbiotics on the HT-29 cell line. Materials and Methods: Through MTT and scratch assay, we investigated the anti-proliferation and anti-migration effects of B. breve and L. rhamnosus postbiotics on HT-29 cells. Furthermore, postbiotic-mediated apoptosis was assessed by analyzing the expression of Bax, Bcl-2, and caspase-3. We also investigated the effects of B. breve postbiotics on the expression of three important genes involved in metastasis, including RSPO2, NGF, and MMP7. Consequently, we validated the expression of selected genes in twelve adenocarcinoma tissues. Results: The results demonstrated the significant impact of postbiotics on HT-29 cells, highlighting their ability to induce anti-proliferation, anti-migration, and apoptosis-related effects. Notably, these effects were more pronounced using B. breve postbiotics than L. rhamnosus. Additionally, B. breve postbiotics could inhibit metastasis through upregulation of RSPO2 while downregulating NGF and MMP7 expression in HT-29 cells. Conclusion: Our research suggests that postbiotic metabolites may be effective biological products for the prevention and treatment of cancer. https://ijm.tums.ac.ir/index.php/ijm/article/view/4340Apoptosis;Bifidobacterium breve;Colorectal cancer;Lactobacillus rhamnosus;Postbiotics;Probiotics |
| spellingShingle | Nafiseh Erfanian Hossein Safarpour Tahmineh Tavakoli Mohammad Ali Mahdiabadi Saeed Nasseri Mohammad Hassan Namaei Investigating the therapeutic potential of Bifidobacterium breve and Lactobacillus rhamnosus postbiotics through apoptosis induction in colorectal HT-29 cancer cells Iranian Journal of Microbiology Apoptosis; Bifidobacterium breve; Colorectal cancer; Lactobacillus rhamnosus; Postbiotics; Probiotics |
| title | Investigating the therapeutic potential of Bifidobacterium breve and Lactobacillus rhamnosus postbiotics through apoptosis induction in colorectal HT-29 cancer cells |
| title_full | Investigating the therapeutic potential of Bifidobacterium breve and Lactobacillus rhamnosus postbiotics through apoptosis induction in colorectal HT-29 cancer cells |
| title_fullStr | Investigating the therapeutic potential of Bifidobacterium breve and Lactobacillus rhamnosus postbiotics through apoptosis induction in colorectal HT-29 cancer cells |
| title_full_unstemmed | Investigating the therapeutic potential of Bifidobacterium breve and Lactobacillus rhamnosus postbiotics through apoptosis induction in colorectal HT-29 cancer cells |
| title_short | Investigating the therapeutic potential of Bifidobacterium breve and Lactobacillus rhamnosus postbiotics through apoptosis induction in colorectal HT-29 cancer cells |
| title_sort | investigating the therapeutic potential of bifidobacterium breve and lactobacillus rhamnosus postbiotics through apoptosis induction in colorectal ht 29 cancer cells |
| topic | Apoptosis; Bifidobacterium breve; Colorectal cancer; Lactobacillus rhamnosus; Postbiotics; Probiotics |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4340 |
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