Co-Occurrence of <i>L. monocytogenes</i> with Other Bacterial Genera and Bacterial Diversity on Cleaned Conveyor Surfaces in a Swine Slaughterhouse
Bacterial pathogens, such as <i>Listeria monocytogenes</i>, can show resistance to disinfection and persistence on working surfaces, permitting them to survive and contaminate food products. Persistence—a complex phenomenon involving interactions between many bacteria within a biofilm—is...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/3/613 |
| _version_ | 1797444627014352896 |
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| author | Tamazight Cherifi Julie Arsenault Sylvain Quessy Philippe Fravalo |
| author_facet | Tamazight Cherifi Julie Arsenault Sylvain Quessy Philippe Fravalo |
| author_sort | Tamazight Cherifi |
| collection | DOAJ |
| description | Bacterial pathogens, such as <i>Listeria monocytogenes</i>, can show resistance to disinfection and persistence on working surfaces, permitting them to survive and contaminate food products. Persistence—a complex phenomenon involving interactions between many bacteria within a biofilm—is modulated by in situ characteristics. This study aimed to describe, in silico, the microbiota identified in a swine slaughterhouse after sanitation procedures to better understand the presence of <i>L. monocytogenes</i> on these surfaces. Molecular tools for characterization of microbial communities were used to assess the relative contribution of different bacteria resulting from this phenomenon, and the 16S rRNA sequencing method was used on samples from meat conveyor belt surfaces collected on four sampling visits to study the co-occurrence between <i>L. monocytogenes</i> and other bacteria. From the background microbiota, a total of six genera were found to be negatively correlated with <i>Listeria</i> spp., suggesting <i>Listeria</i> growth inhibition, competition, or at least an absence of shared habitats. Based on these results, a complete scenario of interactions of <i>Listeria</i> with components of background microbiota was established. This work contributes to identifying avenues that could prevent the growth and persistence of <i>L. monocytogenes</i> on food-processing surfaces. |
| first_indexed | 2024-03-09T13:15:18Z |
| format | Article |
| id | doaj.art-325fa7c19d8f4f2e99d68fa95b244f5a |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T13:15:18Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-325fa7c19d8f4f2e99d68fa95b244f5a2023-11-30T21:36:56ZengMDPI AGMicroorganisms2076-26072022-03-0110361310.3390/microorganisms10030613Co-Occurrence of <i>L. monocytogenes</i> with Other Bacterial Genera and Bacterial Diversity on Cleaned Conveyor Surfaces in a Swine SlaughterhouseTamazight Cherifi0Julie Arsenault1Sylvain Quessy2Philippe Fravalo3Chaire de Recherche en Salubrité des Viandes, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC J2S 2M2, CanadaCentre de Recherche en Infectiologie Porcine et Avicole (CRIPA-FQRNT), Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC J2S 2M2, CanadaChaire de Recherche en Salubrité des Viandes, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC J2S 2M2, CanadaChaire de Recherche en Salubrité des Viandes, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC J2S 2M2, CanadaBacterial pathogens, such as <i>Listeria monocytogenes</i>, can show resistance to disinfection and persistence on working surfaces, permitting them to survive and contaminate food products. Persistence—a complex phenomenon involving interactions between many bacteria within a biofilm—is modulated by in situ characteristics. This study aimed to describe, in silico, the microbiota identified in a swine slaughterhouse after sanitation procedures to better understand the presence of <i>L. monocytogenes</i> on these surfaces. Molecular tools for characterization of microbial communities were used to assess the relative contribution of different bacteria resulting from this phenomenon, and the 16S rRNA sequencing method was used on samples from meat conveyor belt surfaces collected on four sampling visits to study the co-occurrence between <i>L. monocytogenes</i> and other bacteria. From the background microbiota, a total of six genera were found to be negatively correlated with <i>Listeria</i> spp., suggesting <i>Listeria</i> growth inhibition, competition, or at least an absence of shared habitats. Based on these results, a complete scenario of interactions of <i>Listeria</i> with components of background microbiota was established. This work contributes to identifying avenues that could prevent the growth and persistence of <i>L. monocytogenes</i> on food-processing surfaces.https://www.mdpi.com/2076-2607/10/3/613<i>Listeria monocytogenes</i>microbiotaswine slaughterhouse |
| spellingShingle | Tamazight Cherifi Julie Arsenault Sylvain Quessy Philippe Fravalo Co-Occurrence of <i>L. monocytogenes</i> with Other Bacterial Genera and Bacterial Diversity on Cleaned Conveyor Surfaces in a Swine Slaughterhouse Microorganisms <i>Listeria monocytogenes</i> microbiota swine slaughterhouse |
| title | Co-Occurrence of <i>L. monocytogenes</i> with Other Bacterial Genera and Bacterial Diversity on Cleaned Conveyor Surfaces in a Swine Slaughterhouse |
| title_full | Co-Occurrence of <i>L. monocytogenes</i> with Other Bacterial Genera and Bacterial Diversity on Cleaned Conveyor Surfaces in a Swine Slaughterhouse |
| title_fullStr | Co-Occurrence of <i>L. monocytogenes</i> with Other Bacterial Genera and Bacterial Diversity on Cleaned Conveyor Surfaces in a Swine Slaughterhouse |
| title_full_unstemmed | Co-Occurrence of <i>L. monocytogenes</i> with Other Bacterial Genera and Bacterial Diversity on Cleaned Conveyor Surfaces in a Swine Slaughterhouse |
| title_short | Co-Occurrence of <i>L. monocytogenes</i> with Other Bacterial Genera and Bacterial Diversity on Cleaned Conveyor Surfaces in a Swine Slaughterhouse |
| title_sort | co occurrence of i l monocytogenes i with other bacterial genera and bacterial diversity on cleaned conveyor surfaces in a swine slaughterhouse |
| topic | <i>Listeria monocytogenes</i> microbiota swine slaughterhouse |
| url | https://www.mdpi.com/2076-2607/10/3/613 |
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