Functional analysis of the <it>theobroma cacao NPR1 </it>gene in <it>arabidopsis</it>
<p>Abstract</p> <p>Background</p> <p>The <it>Arabidopsis thaliana NPR1 </it>gene encodes a transcription coactivator (NPR1) that plays a major role in the mechanisms regulating plant defense response. After pathogen infection and in response to salicylic aci...
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| Format: | Article |
| Language: | English |
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BMC
2010-11-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/10/248 |
| _version_ | 1819138346943774720 |
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| author | Verica Joseph Liu Yi Maximova Siela N Shi Zi Guiltinan Mark J |
| author_facet | Verica Joseph Liu Yi Maximova Siela N Shi Zi Guiltinan Mark J |
| author_sort | Verica Joseph |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The <it>Arabidopsis thaliana NPR1 </it>gene encodes a transcription coactivator (NPR1) that plays a major role in the mechanisms regulating plant defense response. After pathogen infection and in response to salicylic acid (SA) accumulation, NPR1 translocates from the cytoplasm into the nucleus where it interacts with other transcription factors resulting in increased expression of over 2000 plant defense genes contributing to a pathogen resistance response.</p> <p>Results</p> <p>A putative <it>Theobroma cacao NPR1 </it>cDNA was isolated by RT-PCR using degenerate primers based on homologous sequences from <it>Brassica</it>, <it>Arabidopsis </it>and <it>Carica papaya</it>. The cDNA was used to isolate a genomic clone from <it>Theobroma cacao </it>containing a putative <it>TcNPR1 </it>gene. DNA sequencing revealed the presence of a 4.5 kb coding region containing three introns and encoding a polypeptide of 591 amino acids. The predicted TcNPR1 protein shares 55% identity and 78% similarity to <it>Arabidopsis </it>NPR1, and contains each of the highly conserved functional domains indicative of this class of transcription factors (BTB/POZ and ankyrin repeat protein-protein interaction domains and a nuclear localization sequence (NLS)). To functionally define the <it>TcNPR1 </it>gene, we transferred <it>TcNPR1 </it>into an <it>Arabidopsis npr1 </it>mutant that is highly susceptible to infection by the plant pathogen <it>Pseudomonas syringae </it>pv. tomato DC3000. Driven by the constitutive CaMV35S promoter, the cacao <it>TcNPR1 </it>gene partially complemented the <it>npr1 </it>mutation in transgenic <it>Arabidopsis </it>plants, resulting in 100 fold less bacterial growth in a leaf infection assay. Upon induction with SA, <it>TcNPR1 </it>was shown to translocate into the nucleus of leaf and root cells in a manner identical to <it>Arabidopsis </it>NPR1. Cacao NPR1 was also capable of participating in SA-JA signaling crosstalk, as evidenced by the suppression of JA responsive gene expression in <it>TcNPR1 </it>overexpressing transgenic plants.</p> <p>Conclusion</p> <p>Our data indicate that the <it>TcNPR1 </it>is a functional ortholog of <it>Arabidopsis NPR1</it>, and is likely to play a major role in defense response in cacao. This fundamental knowledge can contribute to breeding of disease resistant cacao varieties through the application of molecular markers or the use of transgenic strategies.</p> |
| first_indexed | 2024-12-22T11:05:19Z |
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| id | doaj.art-326305d7712a4b5d8bd50107208ffe1a |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-22T11:05:19Z |
| publishDate | 2010-11-01 |
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| series | BMC Plant Biology |
| spelling | doaj.art-326305d7712a4b5d8bd50107208ffe1a2022-12-21T18:28:20ZengBMCBMC Plant Biology1471-22292010-11-0110124810.1186/1471-2229-10-248Functional analysis of the <it>theobroma cacao NPR1 </it>gene in <it>arabidopsis</it>Verica JosephLiu YiMaximova Siela NShi ZiGuiltinan Mark J<p>Abstract</p> <p>Background</p> <p>The <it>Arabidopsis thaliana NPR1 </it>gene encodes a transcription coactivator (NPR1) that plays a major role in the mechanisms regulating plant defense response. After pathogen infection and in response to salicylic acid (SA) accumulation, NPR1 translocates from the cytoplasm into the nucleus where it interacts with other transcription factors resulting in increased expression of over 2000 plant defense genes contributing to a pathogen resistance response.</p> <p>Results</p> <p>A putative <it>Theobroma cacao NPR1 </it>cDNA was isolated by RT-PCR using degenerate primers based on homologous sequences from <it>Brassica</it>, <it>Arabidopsis </it>and <it>Carica papaya</it>. The cDNA was used to isolate a genomic clone from <it>Theobroma cacao </it>containing a putative <it>TcNPR1 </it>gene. DNA sequencing revealed the presence of a 4.5 kb coding region containing three introns and encoding a polypeptide of 591 amino acids. The predicted TcNPR1 protein shares 55% identity and 78% similarity to <it>Arabidopsis </it>NPR1, and contains each of the highly conserved functional domains indicative of this class of transcription factors (BTB/POZ and ankyrin repeat protein-protein interaction domains and a nuclear localization sequence (NLS)). To functionally define the <it>TcNPR1 </it>gene, we transferred <it>TcNPR1 </it>into an <it>Arabidopsis npr1 </it>mutant that is highly susceptible to infection by the plant pathogen <it>Pseudomonas syringae </it>pv. tomato DC3000. Driven by the constitutive CaMV35S promoter, the cacao <it>TcNPR1 </it>gene partially complemented the <it>npr1 </it>mutation in transgenic <it>Arabidopsis </it>plants, resulting in 100 fold less bacterial growth in a leaf infection assay. Upon induction with SA, <it>TcNPR1 </it>was shown to translocate into the nucleus of leaf and root cells in a manner identical to <it>Arabidopsis </it>NPR1. Cacao NPR1 was also capable of participating in SA-JA signaling crosstalk, as evidenced by the suppression of JA responsive gene expression in <it>TcNPR1 </it>overexpressing transgenic plants.</p> <p>Conclusion</p> <p>Our data indicate that the <it>TcNPR1 </it>is a functional ortholog of <it>Arabidopsis NPR1</it>, and is likely to play a major role in defense response in cacao. This fundamental knowledge can contribute to breeding of disease resistant cacao varieties through the application of molecular markers or the use of transgenic strategies.</p>http://www.biomedcentral.com/1471-2229/10/248 |
| spellingShingle | Verica Joseph Liu Yi Maximova Siela N Shi Zi Guiltinan Mark J Functional analysis of the <it>theobroma cacao NPR1 </it>gene in <it>arabidopsis</it> BMC Plant Biology |
| title | Functional analysis of the <it>theobroma cacao NPR1 </it>gene in <it>arabidopsis</it> |
| title_full | Functional analysis of the <it>theobroma cacao NPR1 </it>gene in <it>arabidopsis</it> |
| title_fullStr | Functional analysis of the <it>theobroma cacao NPR1 </it>gene in <it>arabidopsis</it> |
| title_full_unstemmed | Functional analysis of the <it>theobroma cacao NPR1 </it>gene in <it>arabidopsis</it> |
| title_short | Functional analysis of the <it>theobroma cacao NPR1 </it>gene in <it>arabidopsis</it> |
| title_sort | functional analysis of the it theobroma cacao npr1 it gene in it arabidopsis it |
| url | http://www.biomedcentral.com/1471-2229/10/248 |
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