The Design and Use of an Optical Mapping System for the Study of Intracardiac Electrical Signaling
Fluorescent optical mapping of electrically active cardiac tissues provides a unique method to examine the excitation wave dynamics of underlying action potentials. Such mapping can be viewed as a bridge between cellular level and organ systems physiology, e.g., by facilitating the development of ad...
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| Format: | Article |
| Language: | English |
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Elsevier
2012-07-01
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| Series: | Indian Pacing and Electrophysiology Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0972629216305216 |
| _version_ | 1819054885736284160 |
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| author | Maneesh Shrivastav, PhD Megan B. Ghai Ashish Singal Paul A. Iaizzo, PhD |
| author_facet | Maneesh Shrivastav, PhD Megan B. Ghai Ashish Singal Paul A. Iaizzo, PhD |
| author_sort | Maneesh Shrivastav, PhD |
| collection | DOAJ |
| description | Fluorescent optical mapping of electrically active cardiac tissues provides a unique method to examine the excitation wave dynamics of underlying action potentials. Such mapping can be viewed as a bridge between cellular level and organ systems physiology, e.g., by facilitating the development of advanced theoretical concepts of arrhythmia. We present the design and use of a high-speed, high-resolution optical mapping system composed entirely of “off the shelf” components. The electrical design integrates a 256 element photodiode array with a 16 bit data acquisition system. Proper grounding and shielding at various stages of the design reduce electromagnetic interference. Our mechanical design provides flexibility in terms of mounting positions and applications (use for whole heart or tissue preparations), while maintaining precise alignment between all optical components. The system software incorporates a user friendly graphical user interface, e.g., spatially recorded action potentials can be represented as intensity graphs or in strip chart format. Thus, this system is capable of displaying cardiac action potentials with high spatiotemporal resolution. Results from cardiac action potential mapping with intact mouse hearts are provided. It should be noted that this system could be readily configured to study isolated myocardial biopsies (e.g., isolated ventricular trabeculae). We describe the details of a versatile, user-friendly system that could be employed for a magnitude of study protocols. |
| first_indexed | 2024-12-21T12:58:44Z |
| format | Article |
| id | doaj.art-326372e8e2c44a1ab1a2cbaa1fdba455 |
| institution | Directory Open Access Journal |
| issn | 0972-6292 |
| language | English |
| last_indexed | 2024-12-21T12:58:44Z |
| publishDate | 2012-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Indian Pacing and Electrophysiology Journal |
| spelling | doaj.art-326372e8e2c44a1ab1a2cbaa1fdba4552022-12-21T19:03:15ZengElsevierIndian Pacing and Electrophysiology Journal0972-62922012-07-0112413815110.1016/S0972-6292(16)30521-6The Design and Use of an Optical Mapping System for the Study of Intracardiac Electrical SignalingManeesh Shrivastav, PhD0Megan B. Ghai1Ashish Singal2Paul A. Iaizzo, PhD3University of Minnesota, Dept of Biomedical Engineering & SurgeryUniversity of Minnesota, Dept of SurgeryUniversity of Minnesota, Dept of Biomedical Engineering & SurgeryUniversity of Minnesota, Dept of SurgeryFluorescent optical mapping of electrically active cardiac tissues provides a unique method to examine the excitation wave dynamics of underlying action potentials. Such mapping can be viewed as a bridge between cellular level and organ systems physiology, e.g., by facilitating the development of advanced theoretical concepts of arrhythmia. We present the design and use of a high-speed, high-resolution optical mapping system composed entirely of “off the shelf” components. The electrical design integrates a 256 element photodiode array with a 16 bit data acquisition system. Proper grounding and shielding at various stages of the design reduce electromagnetic interference. Our mechanical design provides flexibility in terms of mounting positions and applications (use for whole heart or tissue preparations), while maintaining precise alignment between all optical components. The system software incorporates a user friendly graphical user interface, e.g., spatially recorded action potentials can be represented as intensity graphs or in strip chart format. Thus, this system is capable of displaying cardiac action potentials with high spatiotemporal resolution. Results from cardiac action potential mapping with intact mouse hearts are provided. It should be noted that this system could be readily configured to study isolated myocardial biopsies (e.g., isolated ventricular trabeculae). We describe the details of a versatile, user-friendly system that could be employed for a magnitude of study protocols.http://www.sciencedirect.com/science/article/pii/S0972629216305216optical mappingcardiac mappingsignal processingvoltage sensitive dye |
| spellingShingle | Maneesh Shrivastav, PhD Megan B. Ghai Ashish Singal Paul A. Iaizzo, PhD The Design and Use of an Optical Mapping System for the Study of Intracardiac Electrical Signaling Indian Pacing and Electrophysiology Journal optical mapping cardiac mapping signal processing voltage sensitive dye |
| title | The Design and Use of an Optical Mapping System for the Study of Intracardiac Electrical Signaling |
| title_full | The Design and Use of an Optical Mapping System for the Study of Intracardiac Electrical Signaling |
| title_fullStr | The Design and Use of an Optical Mapping System for the Study of Intracardiac Electrical Signaling |
| title_full_unstemmed | The Design and Use of an Optical Mapping System for the Study of Intracardiac Electrical Signaling |
| title_short | The Design and Use of an Optical Mapping System for the Study of Intracardiac Electrical Signaling |
| title_sort | design and use of an optical mapping system for the study of intracardiac electrical signaling |
| topic | optical mapping cardiac mapping signal processing voltage sensitive dye |
| url | http://www.sciencedirect.com/science/article/pii/S0972629216305216 |
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