Spectrophotometric determination of total lactones in Andrographis paniculata Nees
A spectrophotometric method for determination of total lactones in Andrographis paniculata was established by using dinitrobenzoic acid and potassium hydroxide solutions as colour forming agents. The absorbance of the solution was determined at 536 nm. The linearity range was 12-72 × 10-6 g.ml-1. Th...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2005-11-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://www.sjst.psu.ac.th/journal/27-6-pdf/11-Andrographis-paniculata.pdf |
| _version_ | 1818291652042686464 |
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| author | Napaporn Jantakun Penkae Wichitchote Chantana Aromdee |
| author_facet | Napaporn Jantakun Penkae Wichitchote Chantana Aromdee |
| author_sort | Napaporn Jantakun |
| collection | DOAJ |
| description | A spectrophotometric method for determination of total lactones in Andrographis paniculata was established by using dinitrobenzoic acid and potassium hydroxide solutions as colour forming agents. The absorbance of the solution was determined at 536 nm. The linearity range was 12-72 × 10-6 g.ml-1. The detection limit was 1.2 μg, the quantitation limit was 4.23 μg. The intraday variation had an average of slope 6082.97 g.ml-1, % RSD 0.10; an average intercept 0.2786, %RSD 3.66 (n=3). The interday variation had an average of 6146 g.ml-1 with the %RSD of 6.30 and an average intercept 0.2628, %RSD 4.95 (n=4). The coefficients of determination were 0.998-0.999. The total lactones content, calculated as andrographolide, determined by this method was 8.61±0.52% (n=4) and by the official method, Thai Herbal Pharmacopoeia, was 8.12±0.34% (n=2). The results of the two methods do not differ significantly at P=0.05 (P(|t|>0.903 = 0.53) |
| first_indexed | 2024-12-13T02:47:28Z |
| format | Article |
| id | doaj.art-326747684f3f4cc5b2edb58b104dd059 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-13T02:47:28Z |
| publishDate | 2005-11-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-326747684f3f4cc5b2edb58b104dd0592022-12-22T00:02:09ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952005-11-0127612271231Spectrophotometric determination of total lactones in Andrographis paniculata NeesNapaporn JantakunPenkae WichitchoteChantana AromdeeA spectrophotometric method for determination of total lactones in Andrographis paniculata was established by using dinitrobenzoic acid and potassium hydroxide solutions as colour forming agents. The absorbance of the solution was determined at 536 nm. The linearity range was 12-72 × 10-6 g.ml-1. The detection limit was 1.2 μg, the quantitation limit was 4.23 μg. The intraday variation had an average of slope 6082.97 g.ml-1, % RSD 0.10; an average intercept 0.2786, %RSD 3.66 (n=3). The interday variation had an average of 6146 g.ml-1 with the %RSD of 6.30 and an average intercept 0.2628, %RSD 4.95 (n=4). The coefficients of determination were 0.998-0.999. The total lactones content, calculated as andrographolide, determined by this method was 8.61±0.52% (n=4) and by the official method, Thai Herbal Pharmacopoeia, was 8.12±0.34% (n=2). The results of the two methods do not differ significantly at P=0.05 (P(|t|>0.903 = 0.53)http://www.sjst.psu.ac.th/journal/27-6-pdf/11-Andrographis-paniculata.pdfAndrographis paniculatatotal lactonesspectrophotometric determinationcolorimetry |
| spellingShingle | Napaporn Jantakun Penkae Wichitchote Chantana Aromdee Spectrophotometric determination of total lactones in Andrographis paniculata Nees Songklanakarin Journal of Science and Technology (SJST) Andrographis paniculata total lactones spectrophotometric determination colorimetry |
| title | Spectrophotometric determination of total lactones in Andrographis paniculata Nees |
| title_full | Spectrophotometric determination of total lactones in Andrographis paniculata Nees |
| title_fullStr | Spectrophotometric determination of total lactones in Andrographis paniculata Nees |
| title_full_unstemmed | Spectrophotometric determination of total lactones in Andrographis paniculata Nees |
| title_short | Spectrophotometric determination of total lactones in Andrographis paniculata Nees |
| title_sort | spectrophotometric determination of total lactones in andrographis paniculata nees |
| topic | Andrographis paniculata total lactones spectrophotometric determination colorimetry |
| url | http://www.sjst.psu.ac.th/journal/27-6-pdf/11-Andrographis-paniculata.pdf |
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