Graphene FET Array Biosensor Based on ssDNA Aptamer for Ultrasensitive Hg2+ Detection in Environmental Pollutants

Invisible mercury ion is an extremely poisonous environmental pollutant, therefore, a fast and highly sensitive detection method is of significant importance. In this study, a liquid-gated graphene field-effect transistor (GFET) array biosensor (6 × 6 GFETs on the chip) was fabricated and applied fo...

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Main Authors: Jiawei Tu, Ying Gan, Tao Liang, Qiongwen Hu, Qian Wang, Tianling Ren, Qiyong Sun, Hao Wan, Ping Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-08-01
Series:Frontiers in Chemistry
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fchem.2018.00333/full
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author Jiawei Tu
Ying Gan
Tao Liang
Qiongwen Hu
Qian Wang
Tianling Ren
Qiyong Sun
Qiyong Sun
Hao Wan
Ping Wang
author_facet Jiawei Tu
Ying Gan
Tao Liang
Qiongwen Hu
Qian Wang
Tianling Ren
Qiyong Sun
Qiyong Sun
Hao Wan
Ping Wang
author_sort Jiawei Tu
collection DOAJ
description Invisible mercury ion is an extremely poisonous environmental pollutant, therefore, a fast and highly sensitive detection method is of significant importance. In this study, a liquid-gated graphene field-effect transistor (GFET) array biosensor (6 × 6 GFETs on the chip) was fabricated and applied for Hg2+ quantitate detection based on single-stranded DNA (ssDNA) aptamer. The biosensor showed outstanding selectivity to Hg2+ in mixed solutions containing various metal ions. Moreover, the sensing capability of the biosensor was demonstrated by real-time responses and showed a fairly low detection limit of 40 pM, a wide detection ranged from 100 pM to 100 nM and rapid response time below one second. These results suggest that the GFET array biosensor based on ssDNA aptamer offers a simple fabrication procedure and quite fast method for mercury ion contaminant detection and are promising for various analytical applications.
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spelling doaj.art-3268867f73454ba59728b8e14b3ffac02022-12-22T01:42:57ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462018-08-01610.3389/fchem.2018.00333375828Graphene FET Array Biosensor Based on ssDNA Aptamer for Ultrasensitive Hg2+ Detection in Environmental PollutantsJiawei Tu0Ying Gan1Tao Liang2Qiongwen Hu3Qian Wang4Tianling Ren5Qiyong Sun6Qiyong Sun7Hao Wan8Ping Wang9Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, ChinaBiosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, ChinaBiosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, ChinaBiosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, ChinaTsinghua National Laboratory for Information Science and Technology, Tsinghua University, Beijing, ChinaTsinghua National Laboratory for Information Science and Technology, Tsinghua University, Beijing, ChinaKey Laboratory of Healthy & Intelligent Kitchen System Integration of Zhejiang Province, Ningbo, ChinaNingbo Fotile Kitchenware CO. LTD., Bodi Centre, Hangzhou, ChinaBiosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, ChinaBiosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, ChinaInvisible mercury ion is an extremely poisonous environmental pollutant, therefore, a fast and highly sensitive detection method is of significant importance. In this study, a liquid-gated graphene field-effect transistor (GFET) array biosensor (6 × 6 GFETs on the chip) was fabricated and applied for Hg2+ quantitate detection based on single-stranded DNA (ssDNA) aptamer. The biosensor showed outstanding selectivity to Hg2+ in mixed solutions containing various metal ions. Moreover, the sensing capability of the biosensor was demonstrated by real-time responses and showed a fairly low detection limit of 40 pM, a wide detection ranged from 100 pM to 100 nM and rapid response time below one second. These results suggest that the GFET array biosensor based on ssDNA aptamer offers a simple fabrication procedure and quite fast method for mercury ion contaminant detection and are promising for various analytical applications.https://www.frontiersin.org/article/10.3389/fchem.2018.00333/fullbiosensorgraphene FET arrayHg2+ detectionssDNA aptamerultrasensitive detection
spellingShingle Jiawei Tu
Ying Gan
Tao Liang
Qiongwen Hu
Qian Wang
Tianling Ren
Qiyong Sun
Qiyong Sun
Hao Wan
Ping Wang
Graphene FET Array Biosensor Based on ssDNA Aptamer for Ultrasensitive Hg2+ Detection in Environmental Pollutants
Frontiers in Chemistry
biosensor
graphene FET array
Hg2+ detection
ssDNA aptamer
ultrasensitive detection
title Graphene FET Array Biosensor Based on ssDNA Aptamer for Ultrasensitive Hg2+ Detection in Environmental Pollutants
title_full Graphene FET Array Biosensor Based on ssDNA Aptamer for Ultrasensitive Hg2+ Detection in Environmental Pollutants
title_fullStr Graphene FET Array Biosensor Based on ssDNA Aptamer for Ultrasensitive Hg2+ Detection in Environmental Pollutants
title_full_unstemmed Graphene FET Array Biosensor Based on ssDNA Aptamer for Ultrasensitive Hg2+ Detection in Environmental Pollutants
title_short Graphene FET Array Biosensor Based on ssDNA Aptamer for Ultrasensitive Hg2+ Detection in Environmental Pollutants
title_sort graphene fet array biosensor based on ssdna aptamer for ultrasensitive hg2 detection in environmental pollutants
topic biosensor
graphene FET array
Hg2+ detection
ssDNA aptamer
ultrasensitive detection
url https://www.frontiersin.org/article/10.3389/fchem.2018.00333/full
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