Graphene FET Array Biosensor Based on ssDNA Aptamer for Ultrasensitive Hg2+ Detection in Environmental Pollutants
Invisible mercury ion is an extremely poisonous environmental pollutant, therefore, a fast and highly sensitive detection method is of significant importance. In this study, a liquid-gated graphene field-effect transistor (GFET) array biosensor (6 × 6 GFETs on the chip) was fabricated and applied fo...
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Frontiers Media S.A.
2018-08-01
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Series: | Frontiers in Chemistry |
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Online Access: | https://www.frontiersin.org/article/10.3389/fchem.2018.00333/full |
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author | Jiawei Tu Ying Gan Tao Liang Qiongwen Hu Qian Wang Tianling Ren Qiyong Sun Qiyong Sun Hao Wan Ping Wang |
author_facet | Jiawei Tu Ying Gan Tao Liang Qiongwen Hu Qian Wang Tianling Ren Qiyong Sun Qiyong Sun Hao Wan Ping Wang |
author_sort | Jiawei Tu |
collection | DOAJ |
description | Invisible mercury ion is an extremely poisonous environmental pollutant, therefore, a fast and highly sensitive detection method is of significant importance. In this study, a liquid-gated graphene field-effect transistor (GFET) array biosensor (6 × 6 GFETs on the chip) was fabricated and applied for Hg2+ quantitate detection based on single-stranded DNA (ssDNA) aptamer. The biosensor showed outstanding selectivity to Hg2+ in mixed solutions containing various metal ions. Moreover, the sensing capability of the biosensor was demonstrated by real-time responses and showed a fairly low detection limit of 40 pM, a wide detection ranged from 100 pM to 100 nM and rapid response time below one second. These results suggest that the GFET array biosensor based on ssDNA aptamer offers a simple fabrication procedure and quite fast method for mercury ion contaminant detection and are promising for various analytical applications. |
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issn | 2296-2646 |
language | English |
last_indexed | 2024-12-10T15:45:35Z |
publishDate | 2018-08-01 |
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spelling | doaj.art-3268867f73454ba59728b8e14b3ffac02022-12-22T01:42:57ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462018-08-01610.3389/fchem.2018.00333375828Graphene FET Array Biosensor Based on ssDNA Aptamer for Ultrasensitive Hg2+ Detection in Environmental PollutantsJiawei Tu0Ying Gan1Tao Liang2Qiongwen Hu3Qian Wang4Tianling Ren5Qiyong Sun6Qiyong Sun7Hao Wan8Ping Wang9Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, ChinaBiosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, ChinaBiosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, ChinaBiosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, ChinaTsinghua National Laboratory for Information Science and Technology, Tsinghua University, Beijing, ChinaTsinghua National Laboratory for Information Science and Technology, Tsinghua University, Beijing, ChinaKey Laboratory of Healthy & Intelligent Kitchen System Integration of Zhejiang Province, Ningbo, ChinaNingbo Fotile Kitchenware CO. LTD., Bodi Centre, Hangzhou, ChinaBiosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, ChinaBiosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, ChinaInvisible mercury ion is an extremely poisonous environmental pollutant, therefore, a fast and highly sensitive detection method is of significant importance. In this study, a liquid-gated graphene field-effect transistor (GFET) array biosensor (6 × 6 GFETs on the chip) was fabricated and applied for Hg2+ quantitate detection based on single-stranded DNA (ssDNA) aptamer. The biosensor showed outstanding selectivity to Hg2+ in mixed solutions containing various metal ions. Moreover, the sensing capability of the biosensor was demonstrated by real-time responses and showed a fairly low detection limit of 40 pM, a wide detection ranged from 100 pM to 100 nM and rapid response time below one second. These results suggest that the GFET array biosensor based on ssDNA aptamer offers a simple fabrication procedure and quite fast method for mercury ion contaminant detection and are promising for various analytical applications.https://www.frontiersin.org/article/10.3389/fchem.2018.00333/fullbiosensorgraphene FET arrayHg2+ detectionssDNA aptamerultrasensitive detection |
spellingShingle | Jiawei Tu Ying Gan Tao Liang Qiongwen Hu Qian Wang Tianling Ren Qiyong Sun Qiyong Sun Hao Wan Ping Wang Graphene FET Array Biosensor Based on ssDNA Aptamer for Ultrasensitive Hg2+ Detection in Environmental Pollutants Frontiers in Chemistry biosensor graphene FET array Hg2+ detection ssDNA aptamer ultrasensitive detection |
title | Graphene FET Array Biosensor Based on ssDNA Aptamer for Ultrasensitive Hg2+ Detection in Environmental Pollutants |
title_full | Graphene FET Array Biosensor Based on ssDNA Aptamer for Ultrasensitive Hg2+ Detection in Environmental Pollutants |
title_fullStr | Graphene FET Array Biosensor Based on ssDNA Aptamer for Ultrasensitive Hg2+ Detection in Environmental Pollutants |
title_full_unstemmed | Graphene FET Array Biosensor Based on ssDNA Aptamer for Ultrasensitive Hg2+ Detection in Environmental Pollutants |
title_short | Graphene FET Array Biosensor Based on ssDNA Aptamer for Ultrasensitive Hg2+ Detection in Environmental Pollutants |
title_sort | graphene fet array biosensor based on ssdna aptamer for ultrasensitive hg2 detection in environmental pollutants |
topic | biosensor graphene FET array Hg2+ detection ssDNA aptamer ultrasensitive detection |
url | https://www.frontiersin.org/article/10.3389/fchem.2018.00333/full |
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