Probabilities of evoked and spontaneous synaptic transmission at individual active zones: Lessons from Drosophila
Nerve terminals release neuronal transmitters at morphological specializations known as active zones (AZs). Synaptic vesicle fusion at individual AZs is probabilistic, and this property is fundamental for the neuronal information transfer. Until recently, a lack of appropriate tools limited the stud...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnmol.2022.1110538/full |
| _version_ | 1797961535753027584 |
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| author | Maria Bykhovskaia |
| author_facet | Maria Bykhovskaia |
| author_sort | Maria Bykhovskaia |
| collection | DOAJ |
| description | Nerve terminals release neuronal transmitters at morphological specializations known as active zones (AZs). Synaptic vesicle fusion at individual AZs is probabilistic, and this property is fundamental for the neuronal information transfer. Until recently, a lack of appropriate tools limited the studies of stochastic properties of neuronal secretion at individual AZs. However, Drosophila transgenic lines that express postsynaptically tethered Ca2+ sensor GCaMP enabled the visualization of single exocytic event at individual AZs. The present mini-review discusses how this powerful approach enables the investigation of the evoked and spontaneous transmission at single AZs and promotes the understanding of the properties of both release components. |
| first_indexed | 2024-04-11T00:59:33Z |
| format | Article |
| id | doaj.art-326ebe8d026041a582ed4ff6828f7755 |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-04-11T00:59:33Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-326ebe8d026041a582ed4ff6828f77552023-01-04T18:05:57ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992023-01-011510.3389/fnmol.2022.11105381110538Probabilities of evoked and spontaneous synaptic transmission at individual active zones: Lessons from DrosophilaMaria BykhovskaiaNerve terminals release neuronal transmitters at morphological specializations known as active zones (AZs). Synaptic vesicle fusion at individual AZs is probabilistic, and this property is fundamental for the neuronal information transfer. Until recently, a lack of appropriate tools limited the studies of stochastic properties of neuronal secretion at individual AZs. However, Drosophila transgenic lines that express postsynaptically tethered Ca2+ sensor GCaMP enabled the visualization of single exocytic event at individual AZs. The present mini-review discusses how this powerful approach enables the investigation of the evoked and spontaneous transmission at single AZs and promotes the understanding of the properties of both release components.https://www.frontiersin.org/articles/10.3389/fnmol.2022.1110538/fullexocytosiscalciumGCaMPneuromuscular junctionComplexinglutamate |
| spellingShingle | Maria Bykhovskaia Probabilities of evoked and spontaneous synaptic transmission at individual active zones: Lessons from Drosophila Frontiers in Molecular Neuroscience exocytosis calcium GCaMP neuromuscular junction Complexin glutamate |
| title | Probabilities of evoked and spontaneous synaptic transmission at individual active zones: Lessons from Drosophila |
| title_full | Probabilities of evoked and spontaneous synaptic transmission at individual active zones: Lessons from Drosophila |
| title_fullStr | Probabilities of evoked and spontaneous synaptic transmission at individual active zones: Lessons from Drosophila |
| title_full_unstemmed | Probabilities of evoked and spontaneous synaptic transmission at individual active zones: Lessons from Drosophila |
| title_short | Probabilities of evoked and spontaneous synaptic transmission at individual active zones: Lessons from Drosophila |
| title_sort | probabilities of evoked and spontaneous synaptic transmission at individual active zones lessons from drosophila |
| topic | exocytosis calcium GCaMP neuromuscular junction Complexin glutamate |
| url | https://www.frontiersin.org/articles/10.3389/fnmol.2022.1110538/full |
| work_keys_str_mv | AT mariabykhovskaia probabilitiesofevokedandspontaneoussynaptictransmissionatindividualactivezoneslessonsfromdrosophila |