Transmission of NDM-5-Producing and OXA-48-Producing Escherichia coli Sequence Type 648 by International Visitors without Previous Medical Exposure
ABSTRACT Carbapenemase-producing Escherichia coli sequence type (ST) 648 strains were isolated from two international visitors without previous medical exposure from Southeast Asian countries in a hospital in Japan. One isolate, FUJ80154, carried blaNDM-5 in a complex class 1 integron on an IncFIB/F...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2021-12-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.01827-21 |
| _version_ | 1798015770499743744 |
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| author | Sohei Harada Masahiro Suzuki Toshiharu Sasaki Aki Sakurai Masato Inaba Hosoda Takuya Mitsutaka Wakuda Yohei Doi |
| author_facet | Sohei Harada Masahiro Suzuki Toshiharu Sasaki Aki Sakurai Masato Inaba Hosoda Takuya Mitsutaka Wakuda Yohei Doi |
| author_sort | Sohei Harada |
| collection | DOAJ |
| description | ABSTRACT Carbapenemase-producing Escherichia coli sequence type (ST) 648 strains were isolated from two international visitors without previous medical exposure from Southeast Asian countries in a hospital in Japan. One isolate, FUJ80154, carried blaNDM-5 in a complex class 1 integron on an IncFIB/FII plasmid; the other isolate, FUJ80155, carried two copies of blaOXA-48 on the chromosome flanked by IS1R on both sides. The core-genome based-phylogenetic analysis with publicly available genome data of E. coli ST648 carrying blaNDM-5 or blaOXA-48-like demonstrated high genetic similarity between FUJ80154 and NDM-5-prooducing E. coli ST648 strains isolated in South and Southeast Asian countries. On the other hand, no closely related isolates of FUJ80155 were identified. In the absence of prior hospitalization overseas, neither patient had qualified for routine screening of multidrug-resistant organisms, and the isolates were incidentally identified in cultures ordered at the discretion of the treating physician. IMPORTANCE Although patients with history of international hospitalization are often subject to screening for multidrug-resistant organisms, it is unclear whether patients who reside in countries where carbapenemase-producing Enterobacterales (CPE) is endemic but have no history of local hospitalization contribute to the transmission of CPE. In this study, NDM-5-producing and OXA-48-producing Escherichia coli sequence type (ST) 648, a recently recognized high-risk, multidrug-resistant clone, were detected from two overseas visitors without previous medical exposure. The findings of this study suggest that active surveillance culture on admission to hospital may be considered for travelers from countries with endemicity of carbapenem-resistant organisms even without history of local hospitalization and underscore the need to monitor cross-border transmission of high-risk clones, such as carbapenemase-producing E. coli ST648. |
| first_indexed | 2024-04-11T15:40:05Z |
| format | Article |
| id | doaj.art-3270b1adf4584dfeaa61ebe6435541ad |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-04-11T15:40:05Z |
| publishDate | 2021-12-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-3270b1adf4584dfeaa61ebe6435541ad2022-12-22T04:15:50ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972021-12-019310.1128/spectrum.01827-21Transmission of NDM-5-Producing and OXA-48-Producing Escherichia coli Sequence Type 648 by International Visitors without Previous Medical ExposureSohei Harada0Masahiro Suzuki1Toshiharu Sasaki2Aki Sakurai3Masato Inaba4Hosoda Takuya5Mitsutaka Wakuda6Yohei Doi7Department of Infection Control and Prevention, The University of Tokyo Hospital, Hongo, Bunkyo-ku, Tokyo, JapanDepartment of Microbiology, Fujita Health University School of Medicine, Toyoake, Aichi, JapanDepartment of Infectious Diseases, Fujita Health University School of Medicine, Toyoake, Aichi, JapanDepartment of Infectious Diseases, Fujita Health University School of Medicine, Toyoake, Aichi, JapanDepartment of Infectious Diseases, Fujita Health University School of Medicine, Toyoake, Aichi, JapanDepartment of Joint Research Laboratory of Clinical Medicine, Fujita Health University Okazaki Medical Center, Okazaki, Aichi, JapanDepartment of Joint Research Laboratory of Clinical Medicine, Fujita Health University Hospital, Toyoake, Aichi, JapanDepartment of Infectious Diseases, Fujita Health University School of Medicine, Toyoake, Aichi, JapanABSTRACT Carbapenemase-producing Escherichia coli sequence type (ST) 648 strains were isolated from two international visitors without previous medical exposure from Southeast Asian countries in a hospital in Japan. One isolate, FUJ80154, carried blaNDM-5 in a complex class 1 integron on an IncFIB/FII plasmid; the other isolate, FUJ80155, carried two copies of blaOXA-48 on the chromosome flanked by IS1R on both sides. The core-genome based-phylogenetic analysis with publicly available genome data of E. coli ST648 carrying blaNDM-5 or blaOXA-48-like demonstrated high genetic similarity between FUJ80154 and NDM-5-prooducing E. coli ST648 strains isolated in South and Southeast Asian countries. On the other hand, no closely related isolates of FUJ80155 were identified. In the absence of prior hospitalization overseas, neither patient had qualified for routine screening of multidrug-resistant organisms, and the isolates were incidentally identified in cultures ordered at the discretion of the treating physician. IMPORTANCE Although patients with history of international hospitalization are often subject to screening for multidrug-resistant organisms, it is unclear whether patients who reside in countries where carbapenemase-producing Enterobacterales (CPE) is endemic but have no history of local hospitalization contribute to the transmission of CPE. In this study, NDM-5-producing and OXA-48-producing Escherichia coli sequence type (ST) 648, a recently recognized high-risk, multidrug-resistant clone, were detected from two overseas visitors without previous medical exposure. The findings of this study suggest that active surveillance culture on admission to hospital may be considered for travelers from countries with endemicity of carbapenem-resistant organisms even without history of local hospitalization and underscore the need to monitor cross-border transmission of high-risk clones, such as carbapenemase-producing E. coli ST648.https://journals.asm.org/doi/10.1128/spectrum.01827-21Escherichia coliNDM-5OXA-48ST648carbapenemase |
| spellingShingle | Sohei Harada Masahiro Suzuki Toshiharu Sasaki Aki Sakurai Masato Inaba Hosoda Takuya Mitsutaka Wakuda Yohei Doi Transmission of NDM-5-Producing and OXA-48-Producing Escherichia coli Sequence Type 648 by International Visitors without Previous Medical Exposure Microbiology Spectrum Escherichia coli NDM-5 OXA-48 ST648 carbapenemase |
| title | Transmission of NDM-5-Producing and OXA-48-Producing Escherichia coli Sequence Type 648 by International Visitors without Previous Medical Exposure |
| title_full | Transmission of NDM-5-Producing and OXA-48-Producing Escherichia coli Sequence Type 648 by International Visitors without Previous Medical Exposure |
| title_fullStr | Transmission of NDM-5-Producing and OXA-48-Producing Escherichia coli Sequence Type 648 by International Visitors without Previous Medical Exposure |
| title_full_unstemmed | Transmission of NDM-5-Producing and OXA-48-Producing Escherichia coli Sequence Type 648 by International Visitors without Previous Medical Exposure |
| title_short | Transmission of NDM-5-Producing and OXA-48-Producing Escherichia coli Sequence Type 648 by International Visitors without Previous Medical Exposure |
| title_sort | transmission of ndm 5 producing and oxa 48 producing escherichia coli sequence type 648 by international visitors without previous medical exposure |
| topic | Escherichia coli NDM-5 OXA-48 ST648 carbapenemase |
| url | https://journals.asm.org/doi/10.1128/spectrum.01827-21 |
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