Salmonella enterica serovar Choleraesuis vector delivering a dual-antigen expression cassette provides mouse cross-protection against Streptococcus suis serotypes 2, 7, 9, and 1/2
Abstract A universal vaccine protecting against multiple serotypes of Streptococcus suis is urgently needed to improve animal welfare and reduce the consumption of antibiotics. In this study, a dual antigen expression cassette consisting of SS2-SaoA and SS9-Eno was delivered by a recombinant Salmone...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s13567-022-01062-9 |
| _version_ | 1811334683571519488 |
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| author | Yu-an Li Yanni Sun Yang Fu Yuqin Zhang Quan Li Shifeng Wang Huoying Shi |
| author_facet | Yu-an Li Yanni Sun Yang Fu Yuqin Zhang Quan Li Shifeng Wang Huoying Shi |
| author_sort | Yu-an Li |
| collection | DOAJ |
| description | Abstract A universal vaccine protecting against multiple serotypes of Streptococcus suis is urgently needed to improve animal welfare and reduce the consumption of antibiotics. In this study, a dual antigen expression cassette consisting of SS2-SaoA and SS9-Eno was delivered by a recombinant Salmonella Choleraesuis vector to form the vaccine candidate rSC0016(pS-SE). SaoA and Eno were simultaneously synthesized in rSC0016(pS-SE) without affecting the colonization of the recombinant vector in the lymphatic system. In addition, the antiserum of mice immunized with rSC0016(pS-SE) produced a broader and potent opsonophagocytic response against multiple serotypes of S. suis. Finally, rSC0016(pS-SE) provided mice with a 100% protection against a lethal dose of parent S. suis serotype 2 and serotype 9, and provided 90% and 80% protection against heterologous S. suis serotype 7 or 1/2. These values were significantly higher than those obtained with rSC0016(pS-SaoA) or rSC0016(pS-Eno). Together, this study serves as a foundation for developing a universal vaccine against multiple serotypes of S. suis. |
| first_indexed | 2024-04-13T17:11:39Z |
| format | Article |
| id | doaj.art-327193a66e7046e48f8fa888b098f9fd |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-04-13T17:11:39Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-327193a66e7046e48f8fa888b098f9fd2022-12-22T02:38:15ZengBMCVeterinary Research1297-97162022-06-0153111510.1186/s13567-022-01062-9Salmonella enterica serovar Choleraesuis vector delivering a dual-antigen expression cassette provides mouse cross-protection against Streptococcus suis serotypes 2, 7, 9, and 1/2Yu-an Li0Yanni Sun1Yang Fu2Yuqin Zhang3Quan Li4Shifeng Wang5Huoying Shi6College of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityDepartment of Infectious Diseases and Immunology, College of Veterinary Medicine, University of FloridaCollege of Veterinary Medicine, Yangzhou UniversityAbstract A universal vaccine protecting against multiple serotypes of Streptococcus suis is urgently needed to improve animal welfare and reduce the consumption of antibiotics. In this study, a dual antigen expression cassette consisting of SS2-SaoA and SS9-Eno was delivered by a recombinant Salmonella Choleraesuis vector to form the vaccine candidate rSC0016(pS-SE). SaoA and Eno were simultaneously synthesized in rSC0016(pS-SE) without affecting the colonization of the recombinant vector in the lymphatic system. In addition, the antiserum of mice immunized with rSC0016(pS-SE) produced a broader and potent opsonophagocytic response against multiple serotypes of S. suis. Finally, rSC0016(pS-SE) provided mice with a 100% protection against a lethal dose of parent S. suis serotype 2 and serotype 9, and provided 90% and 80% protection against heterologous S. suis serotype 7 or 1/2. These values were significantly higher than those obtained with rSC0016(pS-SaoA) or rSC0016(pS-Eno). Together, this study serves as a foundation for developing a universal vaccine against multiple serotypes of S. suis.https://doi.org/10.1186/s13567-022-01062-9Salmonella Choleraesuis vectorStreptococcus suisdual antigen expression cassettesuniversal vaccine |
| spellingShingle | Yu-an Li Yanni Sun Yang Fu Yuqin Zhang Quan Li Shifeng Wang Huoying Shi Salmonella enterica serovar Choleraesuis vector delivering a dual-antigen expression cassette provides mouse cross-protection against Streptococcus suis serotypes 2, 7, 9, and 1/2 Veterinary Research Salmonella Choleraesuis vector Streptococcus suis dual antigen expression cassettes universal vaccine |
| title | Salmonella enterica serovar Choleraesuis vector delivering a dual-antigen expression cassette provides mouse cross-protection against Streptococcus suis serotypes 2, 7, 9, and 1/2 |
| title_full | Salmonella enterica serovar Choleraesuis vector delivering a dual-antigen expression cassette provides mouse cross-protection against Streptococcus suis serotypes 2, 7, 9, and 1/2 |
| title_fullStr | Salmonella enterica serovar Choleraesuis vector delivering a dual-antigen expression cassette provides mouse cross-protection against Streptococcus suis serotypes 2, 7, 9, and 1/2 |
| title_full_unstemmed | Salmonella enterica serovar Choleraesuis vector delivering a dual-antigen expression cassette provides mouse cross-protection against Streptococcus suis serotypes 2, 7, 9, and 1/2 |
| title_short | Salmonella enterica serovar Choleraesuis vector delivering a dual-antigen expression cassette provides mouse cross-protection against Streptococcus suis serotypes 2, 7, 9, and 1/2 |
| title_sort | salmonella enterica serovar choleraesuis vector delivering a dual antigen expression cassette provides mouse cross protection against streptococcus suis serotypes 2 7 9 and 1 2 |
| topic | Salmonella Choleraesuis vector Streptococcus suis dual antigen expression cassettes universal vaccine |
| url | https://doi.org/10.1186/s13567-022-01062-9 |
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