Antagonistic potential of Bacillus spp. isolates against bacterial pathogens of tomato and fungal pathogen of pepper
In vitro antagonistic potential of eleven isolates of Bacillus spp. against two phytopathogenic bacteria and one fungus was tested in order to identify potential biocontrol agents in vegetable crops. The Bacillus spp. isolates demonstrated different levels of antagonistic effect against the tested p...
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| Format: | Article |
| Language: | English |
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Institute of Pesticides and Environmental Protection
2018-01-01
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| Series: | Pesticidi i Fitomedicina |
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| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1820-3949/2018/1820-39491801009M.pdf |
| _version_ | 1818577845683748864 |
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| author | Milijašević-Marčić Svetlana Todorović Vesna Stanojević Olja Berić Tanja Stanković Slaviša Todorović Biljana Potočnik Ivana |
| author_facet | Milijašević-Marčić Svetlana Todorović Vesna Stanojević Olja Berić Tanja Stanković Slaviša Todorović Biljana Potočnik Ivana |
| author_sort | Milijašević-Marčić Svetlana |
| collection | DOAJ |
| description | In vitro antagonistic potential of eleven isolates of Bacillus spp. against two phytopathogenic bacteria and one fungus was tested in order to identify potential biocontrol agents in vegetable crops. The Bacillus spp. isolates demonstrated different levels of antagonistic effect against the tested pathogenic microorganisms. Data in the study proved Xanthomonas vesicatoria to be more sensitive to Bacillus spp. strains than Clavibacter michiganensis subsp. michiganensis. Ten B. subtilis strains induced growth inhibition of X. vesicatoria, while a strain of B. pumilus did not affect the growth of that bacterium. The largest inhibition zones against X. vesicatoria were induced by strains B-319, B-325 and B-358. The pathogenic strain C. michiganensis subsp. michiganensis was most inhibited by two B. subtilis strains (B-338 and B-348) with mean inhibition zone diameters of up to 20 mm. B. subtilis strain B-319 which was the best in inhibiting X. vesicatoria, showed the lowest inhibitory effect on C. michiganensis subsp. michiganensis. The largest growth inhibition percentage of Verticillium sp. (PGI approximately 70%) was induced by B. subtilis strains B-310 and B-322. The other B. subtilis strains showed PGI values ranging from 45% to 68%, while B. pumilus strain B-335 had the least antagonistic potential (PGI =34.43%) against the pathogen. This study identified at least one suitable biocontrol candidate, B. subtilis strain B-358, as effective in vitro against all three vegetable pathogens. |
| first_indexed | 2024-12-16T06:36:23Z |
| format | Article |
| id | doaj.art-3273b19c92b64b91abc96df651d1102a |
| institution | Directory Open Access Journal |
| issn | 1820-3949 2406-1026 |
| language | English |
| last_indexed | 2024-12-16T06:36:23Z |
| publishDate | 2018-01-01 |
| publisher | Institute of Pesticides and Environmental Protection |
| record_format | Article |
| series | Pesticidi i Fitomedicina |
| spelling | doaj.art-3273b19c92b64b91abc96df651d1102a2022-12-21T22:40:46ZengInstitute of Pesticides and Environmental ProtectionPesticidi i Fitomedicina1820-39492406-10262018-01-013319181820-39491801009MAntagonistic potential of Bacillus spp. isolates against bacterial pathogens of tomato and fungal pathogen of pepperMilijašević-Marčić Svetlana0Todorović Vesna1Stanojević Olja2Berić Tanja3Stanković Slaviša4Todorović Biljana5Potočnik Ivana6Institute of Pesticides and Environmental Protection, BelgradeUniversity of Belgrade, Faculty of Biology, Belgrade,University of Belgrade, Faculty of Biology, Belgrade,University of Belgrade, Faculty of Biology, Belgrade,University of Belgrade, Faculty of Biology, Belgrade,Institute of Pesticides and Environmental Protection, BelgradeInstitute of Pesticides and Environmental Protection, BelgradeIn vitro antagonistic potential of eleven isolates of Bacillus spp. against two phytopathogenic bacteria and one fungus was tested in order to identify potential biocontrol agents in vegetable crops. The Bacillus spp. isolates demonstrated different levels of antagonistic effect against the tested pathogenic microorganisms. Data in the study proved Xanthomonas vesicatoria to be more sensitive to Bacillus spp. strains than Clavibacter michiganensis subsp. michiganensis. Ten B. subtilis strains induced growth inhibition of X. vesicatoria, while a strain of B. pumilus did not affect the growth of that bacterium. The largest inhibition zones against X. vesicatoria were induced by strains B-319, B-325 and B-358. The pathogenic strain C. michiganensis subsp. michiganensis was most inhibited by two B. subtilis strains (B-338 and B-348) with mean inhibition zone diameters of up to 20 mm. B. subtilis strain B-319 which was the best in inhibiting X. vesicatoria, showed the lowest inhibitory effect on C. michiganensis subsp. michiganensis. The largest growth inhibition percentage of Verticillium sp. (PGI approximately 70%) was induced by B. subtilis strains B-310 and B-322. The other B. subtilis strains showed PGI values ranging from 45% to 68%, while B. pumilus strain B-335 had the least antagonistic potential (PGI =34.43%) against the pathogen. This study identified at least one suitable biocontrol candidate, B. subtilis strain B-358, as effective in vitro against all three vegetable pathogens.https://scindeks-clanci.ceon.rs/data/pdf/1820-3949/2018/1820-39491801009M.pdfBiological controlBacillusBacterial pathogensFungal pathogenTomatoPepper |
| spellingShingle | Milijašević-Marčić Svetlana Todorović Vesna Stanojević Olja Berić Tanja Stanković Slaviša Todorović Biljana Potočnik Ivana Antagonistic potential of Bacillus spp. isolates against bacterial pathogens of tomato and fungal pathogen of pepper Pesticidi i Fitomedicina Biological control Bacillus Bacterial pathogens Fungal pathogen Tomato Pepper |
| title | Antagonistic potential of Bacillus spp. isolates against bacterial pathogens of tomato and fungal pathogen of pepper |
| title_full | Antagonistic potential of Bacillus spp. isolates against bacterial pathogens of tomato and fungal pathogen of pepper |
| title_fullStr | Antagonistic potential of Bacillus spp. isolates against bacterial pathogens of tomato and fungal pathogen of pepper |
| title_full_unstemmed | Antagonistic potential of Bacillus spp. isolates against bacterial pathogens of tomato and fungal pathogen of pepper |
| title_short | Antagonistic potential of Bacillus spp. isolates against bacterial pathogens of tomato and fungal pathogen of pepper |
| title_sort | antagonistic potential of bacillus spp isolates against bacterial pathogens of tomato and fungal pathogen of pepper |
| topic | Biological control Bacillus Bacterial pathogens Fungal pathogen Tomato Pepper |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1820-3949/2018/1820-39491801009M.pdf |
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