Molecular Markers for Detecting a Wide Range of Trichoderma spp. that Might Potentially Cause Green Mold in Pleurotus eryngii
In Pleurotus sp., green mold, which is considered a major epidemic, is caused by several Trichoderma species. To develop a rapid molecular marker specific for Trichoderma spp. that potentially cause green mold, eleven Trichoderma species were collected from mushroom farms and the Korean Agricultural...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-07-01
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| Series: | Mycobiology |
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| Online Access: | http://dx.doi.org/10.1080/12298093.2020.1785754 |
| _version_ | 1819060476200353792 |
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| author | Song Hee Lee Hwa Jin Jung Seung-Beom Hong Jong In Choi Jae-San Ryu |
| author_facet | Song Hee Lee Hwa Jin Jung Seung-Beom Hong Jong In Choi Jae-San Ryu |
| author_sort | Song Hee Lee |
| collection | DOAJ |
| description | In Pleurotus sp., green mold, which is considered a major epidemic, is caused by several Trichoderma species. To develop a rapid molecular marker specific for Trichoderma spp. that potentially cause green mold, eleven Trichoderma species were collected from mushroom farms and the Korean Agricultural Culture Collection (KACC). A dominant fungal isolate from a green mold-infected substrate was identified as Trichoderma pleuroticola based on the sequences of its internal transcribed spacer (ITS) and translation elongation factor 1-α (tef1) genes. In artificial inoculation tests, all Trichoderma spp., including T. atroviride, T. cf. virens, T. citrinoviride, T. harzianum, T. koningii, T. longibrachiatum, T. pleurotum, and T. pleuroticola, showed pathogenicity to some extent, and the observed symptoms were soaked mycelia with a red-brown pigment and retarded mycelium regeneration. A molecular marker was developed for the rapid detection of wide range of Trichoderma spp. based on the DNA sequence alignment of the ITS1 and ITS2 regions of Trichoderma spp. The developed primer set detected only Trichoderma spp., and no cross reactivity with edible mushrooms was observed. The detection limits for the PCR assay of T. harzianum (KACC40558), T. pleurotum (KACC44537), and T. pleuroticola (CAF-TP3) were found to be 500, 50, and 5 fg, respectively, and the detection limit for the pathogen-to-host ratio was approximately 1:10,000 (wt/wt). |
| first_indexed | 2024-12-21T14:27:36Z |
| format | Article |
| id | doaj.art-3273d45a1bc64f42841772d7fcd4327f |
| institution | Directory Open Access Journal |
| issn | 1229-8093 2092-9323 |
| language | English |
| last_indexed | 2024-12-21T14:27:36Z |
| publishDate | 2020-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycobiology |
| spelling | doaj.art-3273d45a1bc64f42841772d7fcd4327f2022-12-21T19:00:35ZengTaylor & Francis GroupMycobiology1229-80932092-93232020-07-01001810.1080/12298093.2020.17857541785754Molecular Markers for Detecting a Wide Range of Trichoderma spp. that Might Potentially Cause Green Mold in Pleurotus eryngiiSong Hee Lee0Hwa Jin Jung1Seung-Beom Hong2Jong In Choi3Jae-San Ryu4Department of Mushroom Science, Korea National College of Agriculture and FisheriesDepartment of Mushroom Science, Korea National College of Agriculture and FisheriesKorean Agricultural Culture Collection, Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development AdministrationMushroom Research Institute, GARESDepartment of Mushroom Science, Korea National College of Agriculture and FisheriesIn Pleurotus sp., green mold, which is considered a major epidemic, is caused by several Trichoderma species. To develop a rapid molecular marker specific for Trichoderma spp. that potentially cause green mold, eleven Trichoderma species were collected from mushroom farms and the Korean Agricultural Culture Collection (KACC). A dominant fungal isolate from a green mold-infected substrate was identified as Trichoderma pleuroticola based on the sequences of its internal transcribed spacer (ITS) and translation elongation factor 1-α (tef1) genes. In artificial inoculation tests, all Trichoderma spp., including T. atroviride, T. cf. virens, T. citrinoviride, T. harzianum, T. koningii, T. longibrachiatum, T. pleurotum, and T. pleuroticola, showed pathogenicity to some extent, and the observed symptoms were soaked mycelia with a red-brown pigment and retarded mycelium regeneration. A molecular marker was developed for the rapid detection of wide range of Trichoderma spp. based on the DNA sequence alignment of the ITS1 and ITS2 regions of Trichoderma spp. The developed primer set detected only Trichoderma spp., and no cross reactivity with edible mushrooms was observed. The detection limits for the PCR assay of T. harzianum (KACC40558), T. pleurotum (KACC44537), and T. pleuroticola (CAF-TP3) were found to be 500, 50, and 5 fg, respectively, and the detection limit for the pathogen-to-host ratio was approximately 1:10,000 (wt/wt).http://dx.doi.org/10.1080/12298093.2020.1785754trichodermamolecular markergreen moldpleurotus eryngiipathogenicity |
| spellingShingle | Song Hee Lee Hwa Jin Jung Seung-Beom Hong Jong In Choi Jae-San Ryu Molecular Markers for Detecting a Wide Range of Trichoderma spp. that Might Potentially Cause Green Mold in Pleurotus eryngii Mycobiology trichoderma molecular marker green mold pleurotus eryngii pathogenicity |
| title | Molecular Markers for Detecting a Wide Range of Trichoderma spp. that Might Potentially Cause Green Mold in Pleurotus eryngii |
| title_full | Molecular Markers for Detecting a Wide Range of Trichoderma spp. that Might Potentially Cause Green Mold in Pleurotus eryngii |
| title_fullStr | Molecular Markers for Detecting a Wide Range of Trichoderma spp. that Might Potentially Cause Green Mold in Pleurotus eryngii |
| title_full_unstemmed | Molecular Markers for Detecting a Wide Range of Trichoderma spp. that Might Potentially Cause Green Mold in Pleurotus eryngii |
| title_short | Molecular Markers for Detecting a Wide Range of Trichoderma spp. that Might Potentially Cause Green Mold in Pleurotus eryngii |
| title_sort | molecular markers for detecting a wide range of trichoderma spp that might potentially cause green mold in pleurotus eryngii |
| topic | trichoderma molecular marker green mold pleurotus eryngii pathogenicity |
| url | http://dx.doi.org/10.1080/12298093.2020.1785754 |
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