Differential mRNA Expression Profiling Reveals the Role of MiR-375 in Inflammation of Bovine Mammary Epithelial Cells
MicroRNAs (miRNAs) are a class of small non-coding RNAs that regulate post-transcriptional gene expression and several biological processes. Bovine mammary epithelial cells (bMECs) mediate critical immune responses in the mammary gland and the occurrence of mastitis. Current research focuses on miRN...
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MDPI AG
2022-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/11/1431 |
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| author | Yuhang Li Qichao Hu Zhuoma Luoreng Jian Yang Xingping Wang Yun Ma Dawei Wei |
| author_facet | Yuhang Li Qichao Hu Zhuoma Luoreng Jian Yang Xingping Wang Yun Ma Dawei Wei |
| author_sort | Yuhang Li |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are a class of small non-coding RNAs that regulate post-transcriptional gene expression and several biological processes. Bovine mammary epithelial cells (bMECs) mediate critical immune responses in the mammary gland and the occurrence of mastitis. Current research focuses on miRNA regulation of bMECs, but the miR-375 regulatory mechanism in bMECs is unclear. This study explored the role of miR-375 by profiling the transcriptome of miR-375-silenced bMECs using RNA-seq and identifying differentially expressed mRNAs (DIE-mRNAs). There were 63 DIE-mRNAs, including 48 down-regulated and 15 up-regulated mRNAs between miR-375-silenced bMECs and the controls. The Kyoto encyclopedia of genes and genomes (KEGG) and Gene Ontology (GO) functional analysis showed that the DIE-mRNAs enriched nuclear receptor subfamily 4 group A member 1 (<i>NR4A1</i>) and protein tyrosine phosphatase non-receptor type 5 (<i>PTPN5</i>) anti-inflammatory genes of the mitogen-activated protein kinase (MAPK) signaling pathway. However, they showed an opposite trend to the expression of miR-375 silencing, suggesting that miR-375 promotes bMEC inflammation through the MAPK signaling pathway. The findings of this study provide a new reference for understanding the regulation of bMEC inflammation and cow mastitis. |
| first_indexed | 2024-03-10T01:32:21Z |
| format | Article |
| id | doaj.art-3275d42c5b684540b864afca3ad4cbaa |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T01:32:21Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-3275d42c5b684540b864afca3ad4cbaa2023-11-23T13:38:18ZengMDPI AGAnimals2076-26152022-06-011211143110.3390/ani12111431Differential mRNA Expression Profiling Reveals the Role of MiR-375 in Inflammation of Bovine Mammary Epithelial CellsYuhang Li0Qichao Hu1Zhuoma Luoreng2Jian Yang3Xingping Wang4Yun Ma5Dawei Wei6School of Agriculture, Ningxia University, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, Yinchuan 750021, ChinaMicroRNAs (miRNAs) are a class of small non-coding RNAs that regulate post-transcriptional gene expression and several biological processes. Bovine mammary epithelial cells (bMECs) mediate critical immune responses in the mammary gland and the occurrence of mastitis. Current research focuses on miRNA regulation of bMECs, but the miR-375 regulatory mechanism in bMECs is unclear. This study explored the role of miR-375 by profiling the transcriptome of miR-375-silenced bMECs using RNA-seq and identifying differentially expressed mRNAs (DIE-mRNAs). There were 63 DIE-mRNAs, including 48 down-regulated and 15 up-regulated mRNAs between miR-375-silenced bMECs and the controls. The Kyoto encyclopedia of genes and genomes (KEGG) and Gene Ontology (GO) functional analysis showed that the DIE-mRNAs enriched nuclear receptor subfamily 4 group A member 1 (<i>NR4A1</i>) and protein tyrosine phosphatase non-receptor type 5 (<i>PTPN5</i>) anti-inflammatory genes of the mitogen-activated protein kinase (MAPK) signaling pathway. However, they showed an opposite trend to the expression of miR-375 silencing, suggesting that miR-375 promotes bMEC inflammation through the MAPK signaling pathway. The findings of this study provide a new reference for understanding the regulation of bMEC inflammation and cow mastitis.https://www.mdpi.com/2076-2615/12/11/1431miR-375dairy cowmastitisRNA-seqbMECMAPK signaling pathway |
| spellingShingle | Yuhang Li Qichao Hu Zhuoma Luoreng Jian Yang Xingping Wang Yun Ma Dawei Wei Differential mRNA Expression Profiling Reveals the Role of MiR-375 in Inflammation of Bovine Mammary Epithelial Cells Animals miR-375 dairy cow mastitis RNA-seq bMEC MAPK signaling pathway |
| title | Differential mRNA Expression Profiling Reveals the Role of MiR-375 in Inflammation of Bovine Mammary Epithelial Cells |
| title_full | Differential mRNA Expression Profiling Reveals the Role of MiR-375 in Inflammation of Bovine Mammary Epithelial Cells |
| title_fullStr | Differential mRNA Expression Profiling Reveals the Role of MiR-375 in Inflammation of Bovine Mammary Epithelial Cells |
| title_full_unstemmed | Differential mRNA Expression Profiling Reveals the Role of MiR-375 in Inflammation of Bovine Mammary Epithelial Cells |
| title_short | Differential mRNA Expression Profiling Reveals the Role of MiR-375 in Inflammation of Bovine Mammary Epithelial Cells |
| title_sort | differential mrna expression profiling reveals the role of mir 375 in inflammation of bovine mammary epithelial cells |
| topic | miR-375 dairy cow mastitis RNA-seq bMEC MAPK signaling pathway |
| url | https://www.mdpi.com/2076-2615/12/11/1431 |
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