New genes involved in osmotic stress tolerance in Saccharomyces cerevisiae
Adaptation to changes in osmolarity is fundamental for the survival of living cells, and has implications in food and industrial biotechnology. It has been extensively studied in the yeast Saccharomyces cerevisiae, where the Hog1 stress activated protein kinase was discovered about 20 years ago. Hog...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01545/full |
| _version_ | 1811242877084237824 |
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| author | Ramon Gonzalez Pilar Morales Jordi Tronchoni Gustavo Cordero-Bueso Enrico Vaudano Manuel Quirós Maite Novo Rafael Torres-Pérez Eva Valero |
| author_facet | Ramon Gonzalez Pilar Morales Jordi Tronchoni Gustavo Cordero-Bueso Enrico Vaudano Manuel Quirós Maite Novo Rafael Torres-Pérez Eva Valero |
| author_sort | Ramon Gonzalez |
| collection | DOAJ |
| description | Adaptation to changes in osmolarity is fundamental for the survival of living cells, and has implications in food and industrial biotechnology. It has been extensively studied in the yeast Saccharomyces cerevisiae, where the Hog1 stress activated protein kinase was discovered about 20 years ago. Hog1 is the core of the intracellular signaling pathway that governs the adaptive response to osmotic stress in this species. The main endpoint of this program is synthesis and intracellular retention of glycerol, as a compatible osmolyte. Despite many details of the signaling pathways and yeast responses to osmotic challenges have already been described, genome-wide approaches are contributing to refine our knowledge of yeast adaptation to hypertonic media. In this work, we used a quantitative fitness analysis approach in order to deepen our understanding of the interplay between yeast cells and the osmotic environment. Genetic requirements for proper growth under osmotic stress showed both common and specific features when hypertonic conditions were induced by either glucose or sorbitol. Tolerance to high-glucose content requires mitochondrial function, while defective protein targeting to peroxisome, GID-complex function (involved in negative regulation of gluconeogenesis), or chromatin dynamics, result in poor survival to sorbitol-induced osmotic stress. On the other side, the competitive disadvantage of yeast strains defective in the endomembrane system is relieved by hypertonic conditions. This finding points to the Golgi-endosome system as one of the main cell components negatively affected by hyperosmolarity. Most of the biological processes highlighted in this analysis had not been previously related to osmotic stress but are probably relevant in an ecological and evolutionary context. |
| first_indexed | 2024-04-12T13:57:32Z |
| format | Article |
| id | doaj.art-32762a5b2c7543619a80fe3e60526150 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T13:57:32Z |
| publishDate | 2016-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-32762a5b2c7543619a80fe3e605261502022-12-22T03:30:18ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-09-01710.3389/fmicb.2016.01545218934New genes involved in osmotic stress tolerance in Saccharomyces cerevisiaeRamon Gonzalez0Pilar Morales1Jordi Tronchoni2Gustavo Cordero-Bueso3Enrico Vaudano4Manuel Quirós5Maite Novo6Rafael Torres-Pérez7Eva Valero8CSIC- Universidad de La Rioja- Gobierno de La RiojaCSIC- Universidad de La Rioja- Gobierno de La RiojaCSIC- Universidad de La Rioja- Gobierno de La RiojaUniversidad de CádizCREA-Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia AgrariaEvolva Biotech A/SUniversitat Rovira i VirgiliCSIC- Universidad de La Rioja- Gobierno de La RiojaUniversidad Pablo de OlavideAdaptation to changes in osmolarity is fundamental for the survival of living cells, and has implications in food and industrial biotechnology. It has been extensively studied in the yeast Saccharomyces cerevisiae, where the Hog1 stress activated protein kinase was discovered about 20 years ago. Hog1 is the core of the intracellular signaling pathway that governs the adaptive response to osmotic stress in this species. The main endpoint of this program is synthesis and intracellular retention of glycerol, as a compatible osmolyte. Despite many details of the signaling pathways and yeast responses to osmotic challenges have already been described, genome-wide approaches are contributing to refine our knowledge of yeast adaptation to hypertonic media. In this work, we used a quantitative fitness analysis approach in order to deepen our understanding of the interplay between yeast cells and the osmotic environment. Genetic requirements for proper growth under osmotic stress showed both common and specific features when hypertonic conditions were induced by either glucose or sorbitol. Tolerance to high-glucose content requires mitochondrial function, while defective protein targeting to peroxisome, GID-complex function (involved in negative regulation of gluconeogenesis), or chromatin dynamics, result in poor survival to sorbitol-induced osmotic stress. On the other side, the competitive disadvantage of yeast strains defective in the endomembrane system is relieved by hypertonic conditions. This finding points to the Golgi-endosome system as one of the main cell components negatively affected by hyperosmolarity. Most of the biological processes highlighted in this analysis had not been previously related to osmotic stress but are probably relevant in an ecological and evolutionary context.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01545/fullperoxisomeosmotic stressMitochondrial translationEndomembrane systemGID-complexGolgi-endosome |
| spellingShingle | Ramon Gonzalez Pilar Morales Jordi Tronchoni Gustavo Cordero-Bueso Enrico Vaudano Manuel Quirós Maite Novo Rafael Torres-Pérez Eva Valero New genes involved in osmotic stress tolerance in Saccharomyces cerevisiae Frontiers in Microbiology peroxisome osmotic stress Mitochondrial translation Endomembrane system GID-complex Golgi-endosome |
| title | New genes involved in osmotic stress tolerance in Saccharomyces cerevisiae |
| title_full | New genes involved in osmotic stress tolerance in Saccharomyces cerevisiae |
| title_fullStr | New genes involved in osmotic stress tolerance in Saccharomyces cerevisiae |
| title_full_unstemmed | New genes involved in osmotic stress tolerance in Saccharomyces cerevisiae |
| title_short | New genes involved in osmotic stress tolerance in Saccharomyces cerevisiae |
| title_sort | new genes involved in osmotic stress tolerance in saccharomyces cerevisiae |
| topic | peroxisome osmotic stress Mitochondrial translation Endomembrane system GID-complex Golgi-endosome |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01545/full |
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