Novel usage of intraocular pressure-lowering drugs as wound-healing inhibitors after trabeculectomy with cell culture and animal models
Inhibiting the healing of wounds to ensure that the aqueous humor can drain into the scleral space unimpeded and form a filtering bleb plays a crucial role in determining the success rate of glaucoma surgery. The aim of this study was to investigate the novel use, with cell culture and animal models...
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| Format: | Article |
| Language: | English |
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Wiley
2013-07-01
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| Series: | Kaohsiung Journal of Medical Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1607551X12003312 |
| _version_ | 1819070008604491776 |
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| author | Kwou-Yeung Wu Pei-Cheng Wu Yu-Hung Lai Show-Jen Hong |
| author_facet | Kwou-Yeung Wu Pei-Cheng Wu Yu-Hung Lai Show-Jen Hong |
| author_sort | Kwou-Yeung Wu |
| collection | DOAJ |
| description | Inhibiting the healing of wounds to ensure that the aqueous humor can drain into the scleral space unimpeded and form a filtering bleb plays a crucial role in determining the success rate of glaucoma surgery. The aim of this study was to investigate the novel use, with cell culture and animal models, of some commercial intraocular pressure (IOP)-lowering drugs in inhibiting the healing of fibroblast wounds. The Tenon's fibroblasts of rabbits were cultured to evaluate 13 IOP-lowering drugs for cellular proliferation, collagen formation, and migration. These were measured using [3H]thymidine and [3H]proline uptake, and Transwell chambers. A preservative of benzalkonium chloride (BAK) was initially used, with 0.02% as a maximal original concentration. All of the drugs and the BAK were diluted from original commercial concentrations to 1/10, 1/100, and 1/1000. The more inhibitive drugs screened from the cell cultures were then selected for further short-term application during and after trabeculectomy surgeries had been performed on the rabbits. Expression of the proliferative cell nuclear antigen was immunohistochemically examined 3 and 7 days after surgery. The results revealed that the inhibitive effects of BAK in cellular [3H]thymidine and [3H]proline uptake, and cellular migration were only evident at 0.002% concentrations. Based on the results of the cell cultures, timolol, latanoprost, and unoprostone exhibited a greater inhibitory effect than the other drugs. Moreover, the animal studies showed that latanoprost and unoprostone significantly suppressed the positive expression of proliferative cell nuclear antigen around the operative excision area 7 days after the trabeculectomy surgeries. The results indicate that short-term use of some IOP-lowering drugs, such as latanoprost and unoprostone, may inhibit postoperative wound healing after glaucoma surgery. |
| first_indexed | 2024-12-21T16:59:06Z |
| format | Article |
| id | doaj.art-32778627eaf24bd083ddf016091b4300 |
| institution | Directory Open Access Journal |
| issn | 1607-551X |
| language | English |
| last_indexed | 2024-12-21T16:59:06Z |
| publishDate | 2013-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-32778627eaf24bd083ddf016091b43002022-12-21T18:56:41ZengWileyKaohsiung Journal of Medical Sciences1607-551X2013-07-0129735336110.1016/j.kjms.2012.11.002Novel usage of intraocular pressure-lowering drugs as wound-healing inhibitors after trabeculectomy with cell culture and animal modelsKwou-Yeung Wu0Pei-Cheng Wu1Yu-Hung Lai2Show-Jen Hong3Department of Ophthalmology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, TaiwanDepartment of Veterinary Medicine, College of Agriculture, Chiaya University, Chiaya, TaiwanDepartment of Ophthalmology, Kaohsiung Medical University Hospital, Kaohsiung, TaiwanInstitution of Pharmacology, Department of Pharmacology, College of Medicine, Kaohsiung Medical University, Kaohsiung, TaiwanInhibiting the healing of wounds to ensure that the aqueous humor can drain into the scleral space unimpeded and form a filtering bleb plays a crucial role in determining the success rate of glaucoma surgery. The aim of this study was to investigate the novel use, with cell culture and animal models, of some commercial intraocular pressure (IOP)-lowering drugs in inhibiting the healing of fibroblast wounds. The Tenon's fibroblasts of rabbits were cultured to evaluate 13 IOP-lowering drugs for cellular proliferation, collagen formation, and migration. These were measured using [3H]thymidine and [3H]proline uptake, and Transwell chambers. A preservative of benzalkonium chloride (BAK) was initially used, with 0.02% as a maximal original concentration. All of the drugs and the BAK were diluted from original commercial concentrations to 1/10, 1/100, and 1/1000. The more inhibitive drugs screened from the cell cultures were then selected for further short-term application during and after trabeculectomy surgeries had been performed on the rabbits. Expression of the proliferative cell nuclear antigen was immunohistochemically examined 3 and 7 days after surgery. The results revealed that the inhibitive effects of BAK in cellular [3H]thymidine and [3H]proline uptake, and cellular migration were only evident at 0.002% concentrations. Based on the results of the cell cultures, timolol, latanoprost, and unoprostone exhibited a greater inhibitory effect than the other drugs. Moreover, the animal studies showed that latanoprost and unoprostone significantly suppressed the positive expression of proliferative cell nuclear antigen around the operative excision area 7 days after the trabeculectomy surgeries. The results indicate that short-term use of some IOP-lowering drugs, such as latanoprost and unoprostone, may inhibit postoperative wound healing after glaucoma surgery.http://www.sciencedirect.com/science/article/pii/S1607551X12003312LatanoprostTrabeculectomyUnoprostoneWound healing |
| spellingShingle | Kwou-Yeung Wu Pei-Cheng Wu Yu-Hung Lai Show-Jen Hong Novel usage of intraocular pressure-lowering drugs as wound-healing inhibitors after trabeculectomy with cell culture and animal models Kaohsiung Journal of Medical Sciences Latanoprost Trabeculectomy Unoprostone Wound healing |
| title | Novel usage of intraocular pressure-lowering drugs as wound-healing inhibitors after trabeculectomy with cell culture and animal models |
| title_full | Novel usage of intraocular pressure-lowering drugs as wound-healing inhibitors after trabeculectomy with cell culture and animal models |
| title_fullStr | Novel usage of intraocular pressure-lowering drugs as wound-healing inhibitors after trabeculectomy with cell culture and animal models |
| title_full_unstemmed | Novel usage of intraocular pressure-lowering drugs as wound-healing inhibitors after trabeculectomy with cell culture and animal models |
| title_short | Novel usage of intraocular pressure-lowering drugs as wound-healing inhibitors after trabeculectomy with cell culture and animal models |
| title_sort | novel usage of intraocular pressure lowering drugs as wound healing inhibitors after trabeculectomy with cell culture and animal models |
| topic | Latanoprost Trabeculectomy Unoprostone Wound healing |
| url | http://www.sciencedirect.com/science/article/pii/S1607551X12003312 |
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