Data repurposing from digital home cage monitoring enlightens new perspectives on mouse motor behaviour and reduction principle
Abstract In this longitudinal study we compare between and within-strain variation in the home-cage spatial preference of three widely used and commercially available mice strains—C57BL/6NCrl, BALB/cAnNCrl and CRL:CD1(ICR)—starting from the first hour post cage-change until the next cage-change, for...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-37464-8 |
| _version_ | 1797784683371560960 |
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| author | Sara Fuochi Mara Rigamonti Marcello Raspa Ferdinando Scavizzi Paolo de Girolamo Livia D’Angelo |
| author_facet | Sara Fuochi Mara Rigamonti Marcello Raspa Ferdinando Scavizzi Paolo de Girolamo Livia D’Angelo |
| author_sort | Sara Fuochi |
| collection | DOAJ |
| description | Abstract In this longitudinal study we compare between and within-strain variation in the home-cage spatial preference of three widely used and commercially available mice strains—C57BL/6NCrl, BALB/cAnNCrl and CRL:CD1(ICR)—starting from the first hour post cage-change until the next cage-change, for three consecutive intervals, to further profile the circadian home-cage behavioural phenotypes. Cage-change can be a stressful moment in the life of laboratory mice, since animals are disturbed during the sleeping hours and must then rapidly re-adapt to a pristine environment, leading to disruptions in normal motor patterns. The novelty of this study resides in characterizing new strain-specific biological phenomena, such as activity along the cage walls and frontality, using the vast data reserves generated by previous experimental data, thus introducing the potential and exploring the applicability of data repurposing to enhance Reduction principle when running in vivo studies. Our results, entirely obtained without the use of new animals, demonstrate that also when referring to space preference within the cage, C57BL/6NCrl has a high variability in the behavioural phenotypes from pre-puberty until early adulthood compared to BALB/cAnNCrl, which is confirmed to be socially disaggregated, and CRL:CD1(ICR) which is conversely highly active and socially aggregated. Our data also suggest that a strain-oriented approach is needed when defining frequency of cage-change as well as maximum allowed animal density, which should be revised, ideally under the EU regulatory framework as well, according to the physiological peculiarities of the strains, and always avoiding the “one size fits all” approach. |
| first_indexed | 2024-03-13T00:43:24Z |
| format | Article |
| id | doaj.art-32783f550a4342acb3e8c54a76f4bda9 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T00:43:24Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-32783f550a4342acb3e8c54a76f4bda92023-07-09T11:11:41ZengNature PortfolioScientific Reports2045-23222023-07-0113111010.1038/s41598-023-37464-8Data repurposing from digital home cage monitoring enlightens new perspectives on mouse motor behaviour and reduction principleSara Fuochi0Mara Rigamonti1Marcello Raspa2Ferdinando Scavizzi3Paolo de Girolamo4Livia D’Angelo5Experimental Animal Center, University of BernTecniplast SpANational Research Council, Institute of Biochemistry and Cell Biology (CNR-IBBC/EMMA/Infrafrontier/IMPC), International Campus ‘A. Buzzati-Traverso’National Research Council, Institute of Biochemistry and Cell Biology (CNR-IBBC/EMMA/Infrafrontier/IMPC), International Campus ‘A. Buzzati-Traverso’Department of Veterinary Medicine and Animal Production, University of Naples Federico IIDepartment of Veterinary Medicine and Animal Production, University of Naples Federico IIAbstract In this longitudinal study we compare between and within-strain variation in the home-cage spatial preference of three widely used and commercially available mice strains—C57BL/6NCrl, BALB/cAnNCrl and CRL:CD1(ICR)—starting from the first hour post cage-change until the next cage-change, for three consecutive intervals, to further profile the circadian home-cage behavioural phenotypes. Cage-change can be a stressful moment in the life of laboratory mice, since animals are disturbed during the sleeping hours and must then rapidly re-adapt to a pristine environment, leading to disruptions in normal motor patterns. The novelty of this study resides in characterizing new strain-specific biological phenomena, such as activity along the cage walls and frontality, using the vast data reserves generated by previous experimental data, thus introducing the potential and exploring the applicability of data repurposing to enhance Reduction principle when running in vivo studies. Our results, entirely obtained without the use of new animals, demonstrate that also when referring to space preference within the cage, C57BL/6NCrl has a high variability in the behavioural phenotypes from pre-puberty until early adulthood compared to BALB/cAnNCrl, which is confirmed to be socially disaggregated, and CRL:CD1(ICR) which is conversely highly active and socially aggregated. Our data also suggest that a strain-oriented approach is needed when defining frequency of cage-change as well as maximum allowed animal density, which should be revised, ideally under the EU regulatory framework as well, according to the physiological peculiarities of the strains, and always avoiding the “one size fits all” approach.https://doi.org/10.1038/s41598-023-37464-8 |
| spellingShingle | Sara Fuochi Mara Rigamonti Marcello Raspa Ferdinando Scavizzi Paolo de Girolamo Livia D’Angelo Data repurposing from digital home cage monitoring enlightens new perspectives on mouse motor behaviour and reduction principle Scientific Reports |
| title | Data repurposing from digital home cage monitoring enlightens new perspectives on mouse motor behaviour and reduction principle |
| title_full | Data repurposing from digital home cage monitoring enlightens new perspectives on mouse motor behaviour and reduction principle |
| title_fullStr | Data repurposing from digital home cage monitoring enlightens new perspectives on mouse motor behaviour and reduction principle |
| title_full_unstemmed | Data repurposing from digital home cage monitoring enlightens new perspectives on mouse motor behaviour and reduction principle |
| title_short | Data repurposing from digital home cage monitoring enlightens new perspectives on mouse motor behaviour and reduction principle |
| title_sort | data repurposing from digital home cage monitoring enlightens new perspectives on mouse motor behaviour and reduction principle |
| url | https://doi.org/10.1038/s41598-023-37464-8 |
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