Exploitation of various physio-morphological and biochemical traits for the identification of drought tolerant genotypes in cotton
Abstract Background Drought is one of the limiting factors for quality and quantity of cotton lint in tropical and sub-tropical regions. Therefore, development of drought tolerant cotton genotypes have become indispensable. The identification of drought tolerant genotypes is pre-requisite to develop...
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-023-04441-2 |
| _version_ | 1827634936725635072 |
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| author | Tahreem Arif Muhammad Tanees Chaudhary Sajid Majeed Iqrar Ahmad Rana Zulfiqar Ali Hosam O. Elansary Ihab Mohamed Moussa Sangmi Sun Muhammad Tehseen Azhar |
| author_facet | Tahreem Arif Muhammad Tanees Chaudhary Sajid Majeed Iqrar Ahmad Rana Zulfiqar Ali Hosam O. Elansary Ihab Mohamed Moussa Sangmi Sun Muhammad Tehseen Azhar |
| author_sort | Tahreem Arif |
| collection | DOAJ |
| description | Abstract Background Drought is one of the limiting factors for quality and quantity of cotton lint in tropical and sub-tropical regions. Therefore, development of drought tolerant cotton genotypes have become indispensable. The identification of drought tolerant genotypes is pre-requisite to develop high yielding cultivars suitable for drought affected areas. Methods Forty upland cotton accessions were selected on the basis of their adaptability and yield. The collected germplasm accessions were evaluated at seedling stage on the basis of morphological, physiological and biochemical parameters. The experiment was conducted under controlled conditions in greenhouse where these genotypes were sown under different levels of drought stress by following factorial under completely randomized design. The data were collected at seedling stages for root and shoot lengths, relative leaf water content, excised leaf water losses, peroxidase content and hydrogen peroxide concentrations in leaf tissues. Results The biometrical analysis revealed that germplasm is significantly varied for recorded parameters, likewise interaction of genotypes and water stress was also significantly varied. The cotton germplasm was categorized in eight clusters based on response to water stress. The genotype Cyto-124 exhibited lowest H2O2 content under drought conditions, minimum excised leaf water loss under stress environment was exhibited by genotypes Ali Akber-802 and CEMB-33. Overall, on the basis of morphological and biochemical traits, SL-516 and Cyto-305 were found to be drought tolerant. Genotypes 1852 − 511, Stoneville 15–17 and Delta Pine-55 showed low values for root length, peroxidase activity and higher value for H2O2 contents. On the basis of these finding, these genotypes were declared as drought susceptible. Conclusion The categorization of cotton germplasm indicating the differential response of various parameters under the control and drought stress conditions. The recorded parameters particularly relative leaf water contents and biochemical assays could be utilized to screen large number of germplasm of cotton for water deficit conditions. Besides, the drought tolerant genotypes identified in this research can be utilized in cotton breeding programs for the development of improved cultivars. |
| first_indexed | 2024-03-09T15:21:14Z |
| format | Article |
| id | doaj.art-327aac0e9ce649d1b22008d48cadf3eb |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-09T15:21:14Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-327aac0e9ce649d1b22008d48cadf3eb2023-11-26T12:47:28ZengBMCBMC Plant Biology1471-22292023-10-0123111010.1186/s12870-023-04441-2Exploitation of various physio-morphological and biochemical traits for the identification of drought tolerant genotypes in cottonTahreem Arif0Muhammad Tanees Chaudhary1Sajid Majeed2Iqrar Ahmad Rana3Zulfiqar Ali4Hosam O. Elansary5Ihab Mohamed Moussa6Sangmi Sun7Muhammad Tehseen Azhar8Department of Plant Breeding and Genetics, University of AgricultureDepartment of Plant Breeding and Genetics, University of AgricultureFederal Seed Certification and Registration Department, Ministry of National Food Security and ResearchCenter of Advance Studies/Center of Agricultural Biochemistry and Biotechnology, University of AgricultureDepartment of Plant Breeding and Genetics, University of AgricultureDepartment of Plant Production, College of Food & Agriculture Sciences, King Saud UniversityDepartment of Botany and Microbiology, College of Science, King Saud UniversityDepartment of Biotechnology, Chonnam National UniversityDepartment of Plant Breeding and Genetics, University of AgricultureAbstract Background Drought is one of the limiting factors for quality and quantity of cotton lint in tropical and sub-tropical regions. Therefore, development of drought tolerant cotton genotypes have become indispensable. The identification of drought tolerant genotypes is pre-requisite to develop high yielding cultivars suitable for drought affected areas. Methods Forty upland cotton accessions were selected on the basis of their adaptability and yield. The collected germplasm accessions were evaluated at seedling stage on the basis of morphological, physiological and biochemical parameters. The experiment was conducted under controlled conditions in greenhouse where these genotypes were sown under different levels of drought stress by following factorial under completely randomized design. The data were collected at seedling stages for root and shoot lengths, relative leaf water content, excised leaf water losses, peroxidase content and hydrogen peroxide concentrations in leaf tissues. Results The biometrical analysis revealed that germplasm is significantly varied for recorded parameters, likewise interaction of genotypes and water stress was also significantly varied. The cotton germplasm was categorized in eight clusters based on response to water stress. The genotype Cyto-124 exhibited lowest H2O2 content under drought conditions, minimum excised leaf water loss under stress environment was exhibited by genotypes Ali Akber-802 and CEMB-33. Overall, on the basis of morphological and biochemical traits, SL-516 and Cyto-305 were found to be drought tolerant. Genotypes 1852 − 511, Stoneville 15–17 and Delta Pine-55 showed low values for root length, peroxidase activity and higher value for H2O2 contents. On the basis of these finding, these genotypes were declared as drought susceptible. Conclusion The categorization of cotton germplasm indicating the differential response of various parameters under the control and drought stress conditions. The recorded parameters particularly relative leaf water contents and biochemical assays could be utilized to screen large number of germplasm of cotton for water deficit conditions. Besides, the drought tolerant genotypes identified in this research can be utilized in cotton breeding programs for the development of improved cultivars.https://doi.org/10.1186/s12870-023-04441-2Biochemical assaysBreedingCottonDroughtGermplasmRelative leaf water content |
| spellingShingle | Tahreem Arif Muhammad Tanees Chaudhary Sajid Majeed Iqrar Ahmad Rana Zulfiqar Ali Hosam O. Elansary Ihab Mohamed Moussa Sangmi Sun Muhammad Tehseen Azhar Exploitation of various physio-morphological and biochemical traits for the identification of drought tolerant genotypes in cotton BMC Plant Biology Biochemical assays Breeding Cotton Drought Germplasm Relative leaf water content |
| title | Exploitation of various physio-morphological and biochemical traits for the identification of drought tolerant genotypes in cotton |
| title_full | Exploitation of various physio-morphological and biochemical traits for the identification of drought tolerant genotypes in cotton |
| title_fullStr | Exploitation of various physio-morphological and biochemical traits for the identification of drought tolerant genotypes in cotton |
| title_full_unstemmed | Exploitation of various physio-morphological and biochemical traits for the identification of drought tolerant genotypes in cotton |
| title_short | Exploitation of various physio-morphological and biochemical traits for the identification of drought tolerant genotypes in cotton |
| title_sort | exploitation of various physio morphological and biochemical traits for the identification of drought tolerant genotypes in cotton |
| topic | Biochemical assays Breeding Cotton Drought Germplasm Relative leaf water content |
| url | https://doi.org/10.1186/s12870-023-04441-2 |
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