Acute hyperglycemia prevents dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury
PURPOSE: To investigate the effects of acute hyperglycemia on dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury. METHODS: Sprague-Dawley rats were randomly arranged to the normoglycemic (NG) or hyperglycemic group (HG), with each group further divided into sham (no I/...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2014-12-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001200812&tlng=en |
| _version_ | 1828427819900731392 |
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| author | Huaxin Wang Hui Chen Lei Wang Lin Liu Min Wang Xiuheng Liu |
| author_facet | Huaxin Wang Hui Chen Lei Wang Lin Liu Min Wang Xiuheng Liu |
| author_sort | Huaxin Wang |
| collection | DOAJ |
| description | PURPOSE: To investigate the effects of acute hyperglycemia on dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury. METHODS: Sprague-Dawley rats were randomly arranged to the normoglycemic (NG) or hyperglycemic group (HG), with each group further divided into sham (no I/R injury), I/R (ischemia-reperfusion) and dex (given by dexmedetomidine) groups. Acute hyperglycemia was induced by intraperitoneal injection (i.p.) of 25% glucose (3 g/kg) 45 min before ischemia. Dexmedetomidine (50 μg/kg, i.p.) was administrated 30 min before induction of ischemia. Renal function, histology, apoptosis, expression of Bax, Bcl-2 and phosphorylated AKT (p-AKT) were detected. RESULTS: I/R insult significantly increased the serum levels of blood urea nitrogen and creatinine, apoptotic tubular epithelial cells, expression of Bax and p-AKT, but decreased Bcl-2 expression. All these changes were further enhanced by hyperglycemia (p<0.05). In hyperglycemic condition, there was no statistically difference between the I/R group and Dex group in all the aforementioned detection indexes (p>0.05). CONCLUSION: Acute hyperglycemia attenuates dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury in non-diabetic rats. |
| first_indexed | 2024-12-10T17:08:16Z |
| format | Article |
| id | doaj.art-327d1d32b6554480951d2b68149ea521 |
| institution | Directory Open Access Journal |
| issn | 0102-8650 |
| language | English |
| last_indexed | 2024-12-10T17:08:16Z |
| publishDate | 2014-12-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-327d1d32b6554480951d2b68149ea5212022-12-22T01:40:23ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira0102-86502014-12-01291281281810.1590/S0102-86502014001900008Acute hyperglycemia prevents dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injuryHuaxin WangHui ChenLei WangLin LiuMin WangXiuheng LiuPURPOSE: To investigate the effects of acute hyperglycemia on dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury. METHODS: Sprague-Dawley rats were randomly arranged to the normoglycemic (NG) or hyperglycemic group (HG), with each group further divided into sham (no I/R injury), I/R (ischemia-reperfusion) and dex (given by dexmedetomidine) groups. Acute hyperglycemia was induced by intraperitoneal injection (i.p.) of 25% glucose (3 g/kg) 45 min before ischemia. Dexmedetomidine (50 μg/kg, i.p.) was administrated 30 min before induction of ischemia. Renal function, histology, apoptosis, expression of Bax, Bcl-2 and phosphorylated AKT (p-AKT) were detected. RESULTS: I/R insult significantly increased the serum levels of blood urea nitrogen and creatinine, apoptotic tubular epithelial cells, expression of Bax and p-AKT, but decreased Bcl-2 expression. All these changes were further enhanced by hyperglycemia (p<0.05). In hyperglycemic condition, there was no statistically difference between the I/R group and Dex group in all the aforementioned detection indexes (p>0.05). CONCLUSION: Acute hyperglycemia attenuates dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury in non-diabetic rats.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001200812&tlng=enHyperglycemiaIschemiaReperfusionKidneyDexmedetomidineRats |
| spellingShingle | Huaxin Wang Hui Chen Lei Wang Lin Liu Min Wang Xiuheng Liu Acute hyperglycemia prevents dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury Acta Cirúrgica Brasileira Hyperglycemia Ischemia Reperfusion Kidney Dexmedetomidine Rats |
| title | Acute hyperglycemia prevents dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury |
| title_full | Acute hyperglycemia prevents dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury |
| title_fullStr | Acute hyperglycemia prevents dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury |
| title_full_unstemmed | Acute hyperglycemia prevents dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury |
| title_short | Acute hyperglycemia prevents dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury |
| title_sort | acute hyperglycemia prevents dexmedetomidine induced preconditioning against renal ischemia reperfusion injury |
| topic | Hyperglycemia Ischemia Reperfusion Kidney Dexmedetomidine Rats |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001200812&tlng=en |
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