Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil
Abstract The aims of the present study were to serosurvey dogs, horses, and humans highly exposed to tick bites for anti-Borrelia burgdorferi s.l. antibodies, identify tick species present, and determine risk factors associated with seropositivity in a rural settlement of Paraná State, southern Braz...
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Colégio Brasileiro de Parasitologia Veterinaria
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| Series: | Revista Brasileira de Parasitologia Veterinária |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000400418&lng=en&tlng=en |
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| author | Denise Amaral Gomes Nascimento Rafael Felipe da Costa Vieira Thállitha Samih Wischral Jayme Vieira Roberta dos Santos Toledo Katia Tamekuni Nelson Jessé Rodrigues dos Santos Daniela Dibb Gonçalves Maria Luísa Vieira Alexander Welker Biondo Odilon Vidotto |
| author_facet | Denise Amaral Gomes Nascimento Rafael Felipe da Costa Vieira Thállitha Samih Wischral Jayme Vieira Roberta dos Santos Toledo Katia Tamekuni Nelson Jessé Rodrigues dos Santos Daniela Dibb Gonçalves Maria Luísa Vieira Alexander Welker Biondo Odilon Vidotto |
| author_sort | Denise Amaral Gomes Nascimento |
| collection | DOAJ |
| description | Abstract The aims of the present study were to serosurvey dogs, horses, and humans highly exposed to tick bites for anti-Borrelia burgdorferi s.l. antibodies, identify tick species present, and determine risk factors associated with seropositivity in a rural settlement of Paraná State, southern Brazil. Eighty-seven residents were sampled, along with their 83 dogs and 18 horses, and individual questionnaires were administered. Immunofluorescence antibody test (IFAT) was performed on serum samples and positive samples were subjected to western blot (WB) analysis. Anti-B. burgdorferi antibodies were found in 4/87 (4.6%) humans, 26/83 (31.3%) dogs, and 7/18 (38.9%) horses by IFAT, with 4/4 humans also positive by WB. Ticks identified were mostly from dogs and included 45/67 Rhipicephalus sanguineus, 21/67 Amblyomma ovale, and 1/67 A. cajennense sensu lato. All (34/34) horse ticks were identified as A. cajennense s.l.. No significant association was found when age, gender, or presence of ticks was correlated to seropositivity to Borrelia sp. In conclusion, although anti-Borrelia antibodies have been found in dogs, horses and their owners from the rural settlement, the lack of isolation, molecular characterization, absence of competent vectors and the low specificity of the commercial WB kit used herein may have impaired risk factor analysis. |
| first_indexed | 2024-04-12T19:31:43Z |
| format | Article |
| id | doaj.art-327e6b2001b241b4b4f6d536ef5e2c05 |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-04-12T19:31:43Z |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-327e6b2001b241b4b4f6d536ef5e2c052022-12-22T03:19:19ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-296125441842210.1590/s1984-29612016085S1984-29612016000400418Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern BrazilDenise Amaral Gomes NascimentoRafael Felipe da Costa VieiraThállitha Samih Wischral Jayme VieiraRoberta dos Santos ToledoKatia TamekuniNelson Jessé Rodrigues dos SantosDaniela Dibb GonçalvesMaria Luísa VieiraAlexander Welker BiondoOdilon VidottoAbstract The aims of the present study were to serosurvey dogs, horses, and humans highly exposed to tick bites for anti-Borrelia burgdorferi s.l. antibodies, identify tick species present, and determine risk factors associated with seropositivity in a rural settlement of Paraná State, southern Brazil. Eighty-seven residents were sampled, along with their 83 dogs and 18 horses, and individual questionnaires were administered. Immunofluorescence antibody test (IFAT) was performed on serum samples and positive samples were subjected to western blot (WB) analysis. Anti-B. burgdorferi antibodies were found in 4/87 (4.6%) humans, 26/83 (31.3%) dogs, and 7/18 (38.9%) horses by IFAT, with 4/4 humans also positive by WB. Ticks identified were mostly from dogs and included 45/67 Rhipicephalus sanguineus, 21/67 Amblyomma ovale, and 1/67 A. cajennense sensu lato. All (34/34) horse ticks were identified as A. cajennense s.l.. No significant association was found when age, gender, or presence of ticks was correlated to seropositivity to Borrelia sp. In conclusion, although anti-Borrelia antibodies have been found in dogs, horses and their owners from the rural settlement, the lack of isolation, molecular characterization, absence of competent vectors and the low specificity of the commercial WB kit used herein may have impaired risk factor analysis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000400418&lng=en&tlng=enTeste de imunofluorescência (IFI)doença de Lymesorologiadoenças transmitidas por carrapatoswestern blot |
| spellingShingle | Denise Amaral Gomes Nascimento Rafael Felipe da Costa Vieira Thállitha Samih Wischral Jayme Vieira Roberta dos Santos Toledo Katia Tamekuni Nelson Jessé Rodrigues dos Santos Daniela Dibb Gonçalves Maria Luísa Vieira Alexander Welker Biondo Odilon Vidotto Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil Revista Brasileira de Parasitologia Veterinária Teste de imunofluorescência (IFI) doença de Lyme sorologia doenças transmitidas por carrapatos western blot |
| title | Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil |
| title_full | Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil |
| title_fullStr | Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil |
| title_full_unstemmed | Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil |
| title_short | Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil |
| title_sort | serosurvey of borrelia in dogs horses and humans exposed to ticks in a rural settlement of southern brazil |
| topic | Teste de imunofluorescência (IFI) doença de Lyme sorologia doenças transmitidas por carrapatos western blot |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000400418&lng=en&tlng=en |
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