Inflammation-associated DNA methylation patterns in epithelium of ulcerative colitis
Aberrant DNA methylation patterns have been reported in inflamed tissues and may play a role in disease. We studied DNA methylation and gene expression profiles of purified intestinal epithelial cells from ulcerative colitis patients, comparing inflamed and non-inflamed areas of the colon. We identi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-08-01
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| Series: | Epigenetics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592294.2017.1334023 |
| _version_ | 1797678945050558464 |
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| author | Alan Barnicle Cathal Seoighe John M. Greally Aaron Golden Laurence J. Egan |
| author_facet | Alan Barnicle Cathal Seoighe John M. Greally Aaron Golden Laurence J. Egan |
| author_sort | Alan Barnicle |
| collection | DOAJ |
| description | Aberrant DNA methylation patterns have been reported in inflamed tissues and may play a role in disease. We studied DNA methylation and gene expression profiles of purified intestinal epithelial cells from ulcerative colitis patients, comparing inflamed and non-inflamed areas of the colon. We identified 577 differentially methylated sites (false discovery rate <0.2) mapping to 210 genes. From gene expression data from the same epithelial cells, we identified 62 differentially expressed genes with increased expression in the presence of inflammation at prostate cancer susceptibility genes PRAC1 and PRAC2. Four genes showed inverse correlation between methylation and gene expression; ROR1, GXYLT2, FOXA2, and, notably, RARB, a gene previously identified as a tumor suppressor in colorectal adenocarcinoma as well as breast, lung and prostate cancer. We highlight targeted and specific patterns of DNA methylation and gene expression in epithelial cells from inflamed colon, while challenging the importance of epithelial cells in the pathogenesis of chronic inflammation. |
| first_indexed | 2024-03-11T23:07:15Z |
| format | Article |
| id | doaj.art-327f6a19629e47e8855c4171d939c00e |
| institution | Directory Open Access Journal |
| issn | 1559-2294 1559-2308 |
| language | English |
| last_indexed | 2024-03-11T23:07:15Z |
| publishDate | 2017-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj.art-327f6a19629e47e8855c4171d939c00e2023-09-21T12:43:13ZengTaylor & Francis GroupEpigenetics1559-22941559-23082017-08-0112859160610.1080/15592294.2017.13340231334023Inflammation-associated DNA methylation patterns in epithelium of ulcerative colitisAlan Barnicle0Cathal Seoighe1John M. Greally2Aaron Golden3Laurence J. Egan4Clinical Pharmacology, School of Medicine, National University of IrelandSchool of Mathematics, Statistics and Applied Mathematics, National University of IrelandAlbert Einstein College of Medicine, Morris Park AvenueSchool of Mathematics, Statistics and Applied Mathematics, National University of IrelandClinical Pharmacology, School of Medicine, National University of IrelandAberrant DNA methylation patterns have been reported in inflamed tissues and may play a role in disease. We studied DNA methylation and gene expression profiles of purified intestinal epithelial cells from ulcerative colitis patients, comparing inflamed and non-inflamed areas of the colon. We identified 577 differentially methylated sites (false discovery rate <0.2) mapping to 210 genes. From gene expression data from the same epithelial cells, we identified 62 differentially expressed genes with increased expression in the presence of inflammation at prostate cancer susceptibility genes PRAC1 and PRAC2. Four genes showed inverse correlation between methylation and gene expression; ROR1, GXYLT2, FOXA2, and, notably, RARB, a gene previously identified as a tumor suppressor in colorectal adenocarcinoma as well as breast, lung and prostate cancer. We highlight targeted and specific patterns of DNA methylation and gene expression in epithelial cells from inflamed colon, while challenging the importance of epithelial cells in the pathogenesis of chronic inflammation.http://dx.doi.org/10.1080/15592294.2017.1334023dna methylationintestinal epithelial cellinflammatory bowel diseasetranscriptomeulcerative colitis |
| spellingShingle | Alan Barnicle Cathal Seoighe John M. Greally Aaron Golden Laurence J. Egan Inflammation-associated DNA methylation patterns in epithelium of ulcerative colitis Epigenetics dna methylation intestinal epithelial cell inflammatory bowel disease transcriptome ulcerative colitis |
| title | Inflammation-associated DNA methylation patterns in epithelium of ulcerative colitis |
| title_full | Inflammation-associated DNA methylation patterns in epithelium of ulcerative colitis |
| title_fullStr | Inflammation-associated DNA methylation patterns in epithelium of ulcerative colitis |
| title_full_unstemmed | Inflammation-associated DNA methylation patterns in epithelium of ulcerative colitis |
| title_short | Inflammation-associated DNA methylation patterns in epithelium of ulcerative colitis |
| title_sort | inflammation associated dna methylation patterns in epithelium of ulcerative colitis |
| topic | dna methylation intestinal epithelial cell inflammatory bowel disease transcriptome ulcerative colitis |
| url | http://dx.doi.org/10.1080/15592294.2017.1334023 |
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