Low oxygen levels as a trigger for enhancement of respiratory metabolism in <it>Saccharomyces cerevisiae</it>
<p>Abstract</p> <p>Background</p> <p>The industrially important yeast <it>Saccharomyces cerevisiae </it>is able to grow both in the presence and absence of oxygen. However, the regulation of its metabolism in conditions of intermediate oxygen availability is...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-10-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/10/461 |
| _version_ | 1818447304385888256 |
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| author | Wiebe Marilyn G Pitkänen Juha-Pekka Toivari Mervi Rintala Eija Ruohonen Laura Penttilä Merja |
| author_facet | Wiebe Marilyn G Pitkänen Juha-Pekka Toivari Mervi Rintala Eija Ruohonen Laura Penttilä Merja |
| author_sort | Wiebe Marilyn G |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The industrially important yeast <it>Saccharomyces cerevisiae </it>is able to grow both in the presence and absence of oxygen. However, the regulation of its metabolism in conditions of intermediate oxygen availability is not well characterised. We assessed the effect of oxygen provision on the transcriptome and proteome of <it>S. cerevisiae </it>in glucose-limited chemostat cultivations in anaerobic and aerobic conditions, and with three intermediate (0.5, 1.0 and 2.8% oxygen) levels of oxygen in the feed gas.</p> <p>Results</p> <p>The main differences in the transcriptome were observed in the comparison of fully aerobic, intermediate oxygen and anaerobic conditions, while the transcriptome was generally unchanged in conditions receiving different intermediate levels (0.5, 1.0 or 2.8% O<sub>2</sub>) of oxygen in the feed gas. Comparison of the transcriptome and proteome data suggested post-transcriptional regulation was important, especially in 0.5% oxygen. In the conditions of intermediate oxygen, the genes encoding enzymes of the respiratory pathway were more highly expressed than in either aerobic or anaerobic conditions. A similar trend was also seen in the proteome and in enzyme activities of the TCA cycle. Further, genes encoding proteins of the mitochondrial translation machinery were present at higher levels in all oxygen-limited and anaerobic conditions, compared to fully aerobic conditions.</p> <p>Conclusion</p> <p>Global upregulation of genes encoding components of the respiratory pathway under conditions of intermediate oxygen suggested a regulatory mechanism to control these genes as a response to the need of more efficient energy production. Further, cells grown in three different intermediate oxygen levels were highly similar at the level of transcription, while they differed at the proteome level, suggesting post-transcriptional mechanisms leading to distinct physiological modes of respiro-fermentative metabolism.</p> |
| first_indexed | 2024-12-14T20:01:29Z |
| format | Article |
| id | doaj.art-328204c14b6e446f80255a178cdf72c1 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-14T20:01:29Z |
| publishDate | 2009-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-328204c14b6e446f80255a178cdf72c12022-12-21T22:49:08ZengBMCBMC Genomics1471-21642009-10-0110146110.1186/1471-2164-10-461Low oxygen levels as a trigger for enhancement of respiratory metabolism in <it>Saccharomyces cerevisiae</it>Wiebe Marilyn GPitkänen Juha-PekkaToivari MerviRintala EijaRuohonen LauraPenttilä Merja<p>Abstract</p> <p>Background</p> <p>The industrially important yeast <it>Saccharomyces cerevisiae </it>is able to grow both in the presence and absence of oxygen. However, the regulation of its metabolism in conditions of intermediate oxygen availability is not well characterised. We assessed the effect of oxygen provision on the transcriptome and proteome of <it>S. cerevisiae </it>in glucose-limited chemostat cultivations in anaerobic and aerobic conditions, and with three intermediate (0.5, 1.0 and 2.8% oxygen) levels of oxygen in the feed gas.</p> <p>Results</p> <p>The main differences in the transcriptome were observed in the comparison of fully aerobic, intermediate oxygen and anaerobic conditions, while the transcriptome was generally unchanged in conditions receiving different intermediate levels (0.5, 1.0 or 2.8% O<sub>2</sub>) of oxygen in the feed gas. Comparison of the transcriptome and proteome data suggested post-transcriptional regulation was important, especially in 0.5% oxygen. In the conditions of intermediate oxygen, the genes encoding enzymes of the respiratory pathway were more highly expressed than in either aerobic or anaerobic conditions. A similar trend was also seen in the proteome and in enzyme activities of the TCA cycle. Further, genes encoding proteins of the mitochondrial translation machinery were present at higher levels in all oxygen-limited and anaerobic conditions, compared to fully aerobic conditions.</p> <p>Conclusion</p> <p>Global upregulation of genes encoding components of the respiratory pathway under conditions of intermediate oxygen suggested a regulatory mechanism to control these genes as a response to the need of more efficient energy production. Further, cells grown in three different intermediate oxygen levels were highly similar at the level of transcription, while they differed at the proteome level, suggesting post-transcriptional mechanisms leading to distinct physiological modes of respiro-fermentative metabolism.</p>http://www.biomedcentral.com/1471-2164/10/461 |
| spellingShingle | Wiebe Marilyn G Pitkänen Juha-Pekka Toivari Mervi Rintala Eija Ruohonen Laura Penttilä Merja Low oxygen levels as a trigger for enhancement of respiratory metabolism in <it>Saccharomyces cerevisiae</it> BMC Genomics |
| title | Low oxygen levels as a trigger for enhancement of respiratory metabolism in <it>Saccharomyces cerevisiae</it> |
| title_full | Low oxygen levels as a trigger for enhancement of respiratory metabolism in <it>Saccharomyces cerevisiae</it> |
| title_fullStr | Low oxygen levels as a trigger for enhancement of respiratory metabolism in <it>Saccharomyces cerevisiae</it> |
| title_full_unstemmed | Low oxygen levels as a trigger for enhancement of respiratory metabolism in <it>Saccharomyces cerevisiae</it> |
| title_short | Low oxygen levels as a trigger for enhancement of respiratory metabolism in <it>Saccharomyces cerevisiae</it> |
| title_sort | low oxygen levels as a trigger for enhancement of respiratory metabolism in it saccharomyces cerevisiae it |
| url | http://www.biomedcentral.com/1471-2164/10/461 |
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