Effects of partial substitution of chemical fertilizer with organic manure on the activity of enzyme and soil bacterial communities in the mountain red soil
IntroductionThe partial substitution of chemical fertilizer with organic manure takes on a critical significance to enhancing soil quality and boosting sustainable agricultural development. However, rare research has studied the effects of partial substitution of chemical fertilizer with organic man...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-09-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1234904/full |
| _version_ | 1797692382107402240 |
|---|---|
| author | Zerang Lu Yun Zhou Yun Zhou Yongmei Li Chunpei Li Mei Lu Xuemei Sun Zhizhang Luo Jixia Zhao Maopan Fan |
| author_facet | Zerang Lu Yun Zhou Yun Zhou Yongmei Li Chunpei Li Mei Lu Xuemei Sun Zhizhang Luo Jixia Zhao Maopan Fan |
| author_sort | Zerang Lu |
| collection | DOAJ |
| description | IntroductionThe partial substitution of chemical fertilizer with organic manure takes on a critical significance to enhancing soil quality and boosting sustainable agricultural development. However, rare research has studied the effects of partial substitution of chemical fertilizer with organic manure on soil bacterial community diversity and enzyme activity in maize field in the mountain red soil region of Yunnan.MethodsIn this study, four treatments were set up in which chemical fertilizer (the application rates of N, P2O5 and K2O were 240, 75 and 75 kg·ha−1, respectively) was substituted by 10% (M10), 20% (M20), 30% (M30) and 40% (M40) of organic manure with equal nitrogen, as well as two control treatments of single application of chemical fertilizer (M0) and no fertilization (CK). The maize (Zea mays L.) crop was sown as a test crop in May 2018. The effects of partial substitution of chemical fertilizer with organic manure on soil physicochemical properties, soil bacterial community diversity and enzyme activity were studied.ResultsThe activities of Cellulase (CBH), Invertase (INV) and β-glucosidase (BG) increased with the increase of organic manure substitution ratio. The activities of β-1,4-N-acetylglucosaminidase (NAG), Urease (URE), and leucine aminopeptidase (LAP) also had the same trend, but the highest activities were 159.92 mg·g−1·h−1, 66.82 mg·g−1·h−1 and 143.90 mg·g−1·h−1 at 30% substitution ratio. Compared with CK and M0 treatments, Shannon index increased notably by 82.91%–116.74% and 92.42%–128.01%, respectively, at the organic manure substitution ratio ranging from 10% to 40%. Chao1 and ACE index increased significantly at the organic manure substitution ratio ranging from 10% to 30%. Proteobacteria was the dominant phylum in all treatments, the relative abundance of Proteobacteria decreased as the organic manure substitution ratio increased. Redundancy analysis showed that microbial biomass C was the main factor affecting the bacterial community composition under partial replacement of chemical fertilizer treatment, while Actinobacteria was the main factor affecting the enzyme activity. In addition, the maize yield of M30 and M40 treatments was significantly higher than that of CK and M0-M20 treatments, and the yield of M30 treatment was the highest, reaching 7652.89 kg·ha−1.ConclusionTherefore, the partial substitution of chemical fertilizer with organic manure can improve soil biological characteristics, while increasing bacterial community diversity and soil enzyme activity. Therefore, a thirty percent organic manure substitution was determined as the optimal substitution ratio for maize farmland in the mountain red soil area of Yunnan, China. |
| first_indexed | 2024-03-12T02:27:47Z |
| format | Article |
| id | doaj.art-32883e69110045dda14bb02653d49299 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T02:27:47Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-32883e69110045dda14bb02653d492992023-09-05T11:28:17ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-09-011410.3389/fmicb.2023.12349041234904Effects of partial substitution of chemical fertilizer with organic manure on the activity of enzyme and soil bacterial communities in the mountain red soilZerang Lu0Yun Zhou1Yun Zhou2Yongmei Li3Chunpei Li4Mei Lu5Xuemei Sun6Zhizhang Luo7Jixia Zhao8Maopan Fan9College of Resources and Environment, Yunnan Agricultural University, Kunming, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming, ChinaCollege of Ecology and Environment, Yunnan University, Kunming, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming, ChinaIntroductionThe partial substitution of chemical fertilizer with organic manure takes on a critical significance to enhancing soil quality and boosting sustainable agricultural development. However, rare research has studied the effects of partial substitution of chemical fertilizer with organic manure on soil bacterial community diversity and enzyme activity in maize field in the mountain red soil region of Yunnan.MethodsIn this study, four treatments were set up in which chemical fertilizer (the application rates of N, P2O5 and K2O were 240, 75 and 75 kg·ha−1, respectively) was substituted by 10% (M10), 20% (M20), 30% (M30) and 40% (M40) of organic manure with equal nitrogen, as well as two control treatments of single application of chemical fertilizer (M0) and no fertilization (CK). The maize (Zea mays L.) crop was sown as a test crop in May 2018. The effects of partial substitution of chemical fertilizer with organic manure on soil physicochemical properties, soil bacterial community diversity and enzyme activity were studied.ResultsThe activities of Cellulase (CBH), Invertase (INV) and β-glucosidase (BG) increased with the increase of organic manure substitution ratio. The activities of β-1,4-N-acetylglucosaminidase (NAG), Urease (URE), and leucine aminopeptidase (LAP) also had the same trend, but the highest activities were 159.92 mg·g−1·h−1, 66.82 mg·g−1·h−1 and 143.90 mg·g−1·h−1 at 30% substitution ratio. Compared with CK and M0 treatments, Shannon index increased notably by 82.91%–116.74% and 92.42%–128.01%, respectively, at the organic manure substitution ratio ranging from 10% to 40%. Chao1 and ACE index increased significantly at the organic manure substitution ratio ranging from 10% to 30%. Proteobacteria was the dominant phylum in all treatments, the relative abundance of Proteobacteria decreased as the organic manure substitution ratio increased. Redundancy analysis showed that microbial biomass C was the main factor affecting the bacterial community composition under partial replacement of chemical fertilizer treatment, while Actinobacteria was the main factor affecting the enzyme activity. In addition, the maize yield of M30 and M40 treatments was significantly higher than that of CK and M0-M20 treatments, and the yield of M30 treatment was the highest, reaching 7652.89 kg·ha−1.ConclusionTherefore, the partial substitution of chemical fertilizer with organic manure can improve soil biological characteristics, while increasing bacterial community diversity and soil enzyme activity. Therefore, a thirty percent organic manure substitution was determined as the optimal substitution ratio for maize farmland in the mountain red soil area of Yunnan, China.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1234904/fullfertilizermaizesoil propertiessoil bacterial communitysoil enzyme activitymountain red soil |
| spellingShingle | Zerang Lu Yun Zhou Yun Zhou Yongmei Li Chunpei Li Mei Lu Xuemei Sun Zhizhang Luo Jixia Zhao Maopan Fan Effects of partial substitution of chemical fertilizer with organic manure on the activity of enzyme and soil bacterial communities in the mountain red soil Frontiers in Microbiology fertilizer maize soil properties soil bacterial community soil enzyme activity mountain red soil |
| title | Effects of partial substitution of chemical fertilizer with organic manure on the activity of enzyme and soil bacterial communities in the mountain red soil |
| title_full | Effects of partial substitution of chemical fertilizer with organic manure on the activity of enzyme and soil bacterial communities in the mountain red soil |
| title_fullStr | Effects of partial substitution of chemical fertilizer with organic manure on the activity of enzyme and soil bacterial communities in the mountain red soil |
| title_full_unstemmed | Effects of partial substitution of chemical fertilizer with organic manure on the activity of enzyme and soil bacterial communities in the mountain red soil |
| title_short | Effects of partial substitution of chemical fertilizer with organic manure on the activity of enzyme and soil bacterial communities in the mountain red soil |
| title_sort | effects of partial substitution of chemical fertilizer with organic manure on the activity of enzyme and soil bacterial communities in the mountain red soil |
| topic | fertilizer maize soil properties soil bacterial community soil enzyme activity mountain red soil |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1234904/full |
| work_keys_str_mv | AT zeranglu effectsofpartialsubstitutionofchemicalfertilizerwithorganicmanureontheactivityofenzymeandsoilbacterialcommunitiesinthemountainredsoil AT yunzhou effectsofpartialsubstitutionofchemicalfertilizerwithorganicmanureontheactivityofenzymeandsoilbacterialcommunitiesinthemountainredsoil AT yunzhou effectsofpartialsubstitutionofchemicalfertilizerwithorganicmanureontheactivityofenzymeandsoilbacterialcommunitiesinthemountainredsoil AT yongmeili effectsofpartialsubstitutionofchemicalfertilizerwithorganicmanureontheactivityofenzymeandsoilbacterialcommunitiesinthemountainredsoil AT chunpeili effectsofpartialsubstitutionofchemicalfertilizerwithorganicmanureontheactivityofenzymeandsoilbacterialcommunitiesinthemountainredsoil AT meilu effectsofpartialsubstitutionofchemicalfertilizerwithorganicmanureontheactivityofenzymeandsoilbacterialcommunitiesinthemountainredsoil AT xuemeisun effectsofpartialsubstitutionofchemicalfertilizerwithorganicmanureontheactivityofenzymeandsoilbacterialcommunitiesinthemountainredsoil AT zhizhangluo effectsofpartialsubstitutionofchemicalfertilizerwithorganicmanureontheactivityofenzymeandsoilbacterialcommunitiesinthemountainredsoil AT jixiazhao effectsofpartialsubstitutionofchemicalfertilizerwithorganicmanureontheactivityofenzymeandsoilbacterialcommunitiesinthemountainredsoil AT maopanfan effectsofpartialsubstitutionofchemicalfertilizerwithorganicmanureontheactivityofenzymeandsoilbacterialcommunitiesinthemountainredsoil |