Gemmatimonas groenlandica sp. nov. Is an Aerobic Anoxygenic Phototroph in the Phylum Gemmatimonadetes
The bacterial phylum Gemmatimonadetes contains members capable of performing bacteriochlorophyll-based phototrophy (chlorophototrophy). However, only one strain of chlorophototrophic Gemmatimonadetes bacteria (CGB) has been isolated to date, hampering our further understanding of their photoheterotr...
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.606612/full |
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| author | Yonghui Zeng Yonghui Zeng Nupur Naicheng Wu Anne Mette Madsen Xihan Chen Alastair T. Gardiner Michal Koblížek |
| author_facet | Yonghui Zeng Yonghui Zeng Nupur Naicheng Wu Anne Mette Madsen Xihan Chen Alastair T. Gardiner Michal Koblížek |
| author_sort | Yonghui Zeng |
| collection | DOAJ |
| description | The bacterial phylum Gemmatimonadetes contains members capable of performing bacteriochlorophyll-based phototrophy (chlorophototrophy). However, only one strain of chlorophototrophic Gemmatimonadetes bacteria (CGB) has been isolated to date, hampering our further understanding of their photoheterotrophic lifestyle and the evolution of phototrophy in CGB. By combining a culturomics strategy with a rapid screening technique for chlorophototrophs, we report the isolation of a new member of CGB, Gemmatimonas (G.) groenlandica sp. nov., from the surface water of a stream in the Zackenberg Valley in High Arctic Greenland. Distinct from the microaerophilic G. phototrophica strain AP64T, G. groenlandica strain TET16T is a strictly aerobic anoxygenic phototroph, lacking many oxygen-independent enzymes while possessing an expanded arsenal for coping with oxidative stresses. Its pigment composition and infra-red absorption properties are also different from G. phototrophica, indicating that it possesses a different photosystem apparatus. The complete genome sequence of G. groenlandica reveals unique and conserved features in the photosynthesis gene clusters of CGB. We further analyzed metagenome-assembled genomes of CGB obtained from soil and glacier metagenomes from Northeast Greenland, revealing a wide distribution pattern of CGB beyond the stream water investigated. |
| first_indexed | 2024-04-12T22:08:20Z |
| format | Article |
| id | doaj.art-3289509704324a039f14174681bbf659 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T22:08:20Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-3289509704324a039f14174681bbf6592022-12-22T03:14:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-01-011110.3389/fmicb.2020.606612606612Gemmatimonas groenlandica sp. nov. Is an Aerobic Anoxygenic Phototroph in the Phylum GemmatimonadetesYonghui Zeng0Yonghui Zeng1 Nupur2Naicheng Wu3Anne Mette Madsen4Xihan Chen5Alastair T. Gardiner6Michal Koblížek7Department of Environmental Science, Aarhus University, Roskilde, DenmarkAarhus Institute of Advanced Studies, Aarhus University, Aarhus, DenmarkCentre Algatech, Institute of Microbiology CAS, Třeboň, CzechiaDepartment of Geography and Spatial Information Techniques, Center for Land and Marine Spatial Utilization and Governance Research, Ningbo University, Ningbo, ChinaThe National Research Centre for the Working Environment, Copenhagen, DenmarkDepartment of Engineering, Aarhus University, Aarhus, DenmarkCentre Algatech, Institute of Microbiology CAS, Třeboň, CzechiaCentre Algatech, Institute of Microbiology CAS, Třeboň, CzechiaThe bacterial phylum Gemmatimonadetes contains members capable of performing bacteriochlorophyll-based phototrophy (chlorophototrophy). However, only one strain of chlorophototrophic Gemmatimonadetes bacteria (CGB) has been isolated to date, hampering our further understanding of their photoheterotrophic lifestyle and the evolution of phototrophy in CGB. By combining a culturomics strategy with a rapid screening technique for chlorophototrophs, we report the isolation of a new member of CGB, Gemmatimonas (G.) groenlandica sp. nov., from the surface water of a stream in the Zackenberg Valley in High Arctic Greenland. Distinct from the microaerophilic G. phototrophica strain AP64T, G. groenlandica strain TET16T is a strictly aerobic anoxygenic phototroph, lacking many oxygen-independent enzymes while possessing an expanded arsenal for coping with oxidative stresses. Its pigment composition and infra-red absorption properties are also different from G. phototrophica, indicating that it possesses a different photosystem apparatus. The complete genome sequence of G. groenlandica reveals unique and conserved features in the photosynthesis gene clusters of CGB. We further analyzed metagenome-assembled genomes of CGB obtained from soil and glacier metagenomes from Northeast Greenland, revealing a wide distribution pattern of CGB beyond the stream water investigated.https://www.frontiersin.org/articles/10.3389/fmicb.2020.606612/fullMALDI-TOF MSbacterial isolationphototrophyGemmatimonadetesoligotrophic environment |
| spellingShingle | Yonghui Zeng Yonghui Zeng Nupur Naicheng Wu Anne Mette Madsen Xihan Chen Alastair T. Gardiner Michal Koblížek Gemmatimonas groenlandica sp. nov. Is an Aerobic Anoxygenic Phototroph in the Phylum Gemmatimonadetes Frontiers in Microbiology MALDI-TOF MS bacterial isolation phototrophy Gemmatimonadetes oligotrophic environment |
| title | Gemmatimonas groenlandica sp. nov. Is an Aerobic Anoxygenic Phototroph in the Phylum Gemmatimonadetes |
| title_full | Gemmatimonas groenlandica sp. nov. Is an Aerobic Anoxygenic Phototroph in the Phylum Gemmatimonadetes |
| title_fullStr | Gemmatimonas groenlandica sp. nov. Is an Aerobic Anoxygenic Phototroph in the Phylum Gemmatimonadetes |
| title_full_unstemmed | Gemmatimonas groenlandica sp. nov. Is an Aerobic Anoxygenic Phototroph in the Phylum Gemmatimonadetes |
| title_short | Gemmatimonas groenlandica sp. nov. Is an Aerobic Anoxygenic Phototroph in the Phylum Gemmatimonadetes |
| title_sort | gemmatimonas groenlandica sp nov is an aerobic anoxygenic phototroph in the phylum gemmatimonadetes |
| topic | MALDI-TOF MS bacterial isolation phototrophy Gemmatimonadetes oligotrophic environment |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.606612/full |
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