Mechanical spinal cord transection in larval zebrafish and subsequent whole-mount histological processing
Summary: Zebrafish regenerate their spinal cord after injury, both at larval and adult stages. Larval zebrafish have emerged as a powerful model system to study spinal cord injury and regeneration due to their high optical transparency for in vivo imaging, amenability to high-throughput analysis, an...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721007991 |
| _version_ | 1818320903999586304 |
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| author | Nora John Julia Kolb Daniel Wehner |
| author_facet | Nora John Julia Kolb Daniel Wehner |
| author_sort | Nora John |
| collection | DOAJ |
| description | Summary: Zebrafish regenerate their spinal cord after injury, both at larval and adult stages. Larval zebrafish have emerged as a powerful model system to study spinal cord injury and regeneration due to their high optical transparency for in vivo imaging, amenability to high-throughput analysis, and rapid regeneration time. Here, we describe a protocol for the mechanical transection of the larval zebrafish spinal cord, followed by whole-mount tissue processing for in situ hybridization and immunohistochemistry to elucidate principles of regeneration.For complete details on the use and execution of this protocol, please refer to Wehner et al. (2017) and Tsata et al. (2021). |
| first_indexed | 2024-12-13T10:32:25Z |
| format | Article |
| id | doaj.art-3289a95fba934dc6888b048b4c9df0fe |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-13T10:32:25Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-3289a95fba934dc6888b048b4c9df0fe2022-12-21T23:50:50ZengElsevierSTAR Protocols2666-16672022-03-0131101093Mechanical spinal cord transection in larval zebrafish and subsequent whole-mount histological processingNora John0Julia Kolb1Daniel Wehner2Max Planck Institute for the Science of Light, 91058 Erlangen, Germany; Max-Planck-Zentrum für Physik und Medizin, 91058 Erlangen, Germany; Department of Biology, Friedrich-Alexander-University Erlangen-Nürnberg, 91058 Erlangen, GermanyMax Planck Institute for the Science of Light, 91058 Erlangen, Germany; Max-Planck-Zentrum für Physik und Medizin, 91058 Erlangen, Germany; Department of Biology, Friedrich-Alexander-University Erlangen-Nürnberg, 91058 Erlangen, GermanyMax Planck Institute for the Science of Light, 91058 Erlangen, Germany; Max-Planck-Zentrum für Physik und Medizin, 91058 Erlangen, Germany; Corresponding authorSummary: Zebrafish regenerate their spinal cord after injury, both at larval and adult stages. Larval zebrafish have emerged as a powerful model system to study spinal cord injury and regeneration due to their high optical transparency for in vivo imaging, amenability to high-throughput analysis, and rapid regeneration time. Here, we describe a protocol for the mechanical transection of the larval zebrafish spinal cord, followed by whole-mount tissue processing for in situ hybridization and immunohistochemistry to elucidate principles of regeneration.For complete details on the use and execution of this protocol, please refer to Wehner et al. (2017) and Tsata et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721007991Developmental biologyMicroscopyModel OrganismsNeuroscience |
| spellingShingle | Nora John Julia Kolb Daniel Wehner Mechanical spinal cord transection in larval zebrafish and subsequent whole-mount histological processing STAR Protocols Developmental biology Microscopy Model Organisms Neuroscience |
| title | Mechanical spinal cord transection in larval zebrafish and subsequent whole-mount histological processing |
| title_full | Mechanical spinal cord transection in larval zebrafish and subsequent whole-mount histological processing |
| title_fullStr | Mechanical spinal cord transection in larval zebrafish and subsequent whole-mount histological processing |
| title_full_unstemmed | Mechanical spinal cord transection in larval zebrafish and subsequent whole-mount histological processing |
| title_short | Mechanical spinal cord transection in larval zebrafish and subsequent whole-mount histological processing |
| title_sort | mechanical spinal cord transection in larval zebrafish and subsequent whole mount histological processing |
| topic | Developmental biology Microscopy Model Organisms Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721007991 |
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