Expression of the G1 epitope of bovine ephemeral fever virus G glycoprotein in eukaryotic cells
The envelope glycoprotein (protein G) of bovine ephemeral fever virus (BEFV) has been identified as a plausible vaccine candidate against the BEF disease. In the present study, G1 epitope of the G gly-coprotein gene was cloned in an eukaryotic expression vector, pcDNA3.1(+), under the control of the...
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria
2018-06-01
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| Series: | Bulgarian Journal of Veterinary Medicine |
| Subjects: | |
| Online Access: | http://tru.uni-sz.bg/bjvm/BJVM%20June%202018%20p.176-185.pdf |
| _version_ | 1819104728993234944 |
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| author | R. Pasandideh M.T. Beigi Nassiri M.R.Seyfi Abad Shapouri J. Fayazi H. Roshanfekr M. Lotfi |
| author_facet | R. Pasandideh M.T. Beigi Nassiri M.R.Seyfi Abad Shapouri J. Fayazi H. Roshanfekr M. Lotfi |
| author_sort | R. Pasandideh |
| collection | DOAJ |
| description | The envelope glycoprotein (protein G) of bovine ephemeral fever virus (BEFV) has been identified as a plausible vaccine candidate against the BEF disease. In the present study, G1 epitope of the G gly-coprotein gene was cloned in an eukaryotic expression vector, pcDNA3.1(+), under the control of the human cytomegalovirus (CMV) promoter. The pcDNA3.1-G1 construct was transfected into human embryonic kidney 293 (HEK 293) cell line and the expression efficiency was verified by immunofluorescence staining of transfected cells and Western blot analysis. The results indicated that G1 protein was expressed by the recombinant pcDNA3.1-G1 construct in the transfected cells. The recombinant plasmid constructed in this study can be used as a DNA vaccine to evaluate its potential for immunogenicity and protection against BEF virus in animal models. |
| first_indexed | 2024-12-22T02:10:58Z |
| format | Article |
| id | doaj.art-3289fc661f1e4cc4942c0b4d6ee125f7 |
| institution | Directory Open Access Journal |
| issn | 1311-1477 1313-3543 |
| language | English |
| last_indexed | 2024-12-22T02:10:58Z |
| publishDate | 2018-06-01 |
| publisher | Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria |
| record_format | Article |
| series | Bulgarian Journal of Veterinary Medicine |
| spelling | doaj.art-3289fc661f1e4cc4942c0b4d6ee125f72022-12-21T18:42:23ZengFaculty of Veterinary Medicine, Trakia University, Stara Zagora, BulgariaBulgarian Journal of Veterinary Medicine1311-14771313-35432018-06-0121217618510.15547/bjvm.1061Expression of the G1 epitope of bovine ephemeral fever virus G glycoprotein in eukaryotic cellsR. Pasandideh0M.T. Beigi Nassiri1M.R.Seyfi Abad Shapouri2J. Fayazi3H. Roshanfekr 4M. Lotfi5Faculty of Animal and Food Science, Ramin Agriculture and Natural Resources University of Khuzestan, Mollasani, IranFaculty of Animal and Food Science, Ramin Agriculture and Natural Resources University of Khuzestan, Mollasani, IranFaculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranFaculty of Animal and Food Science, Ramin Agriculture and Natural Resources University of Khuzestan, Mollasani, IranFaculty of Animal and Food Science, Ramin Agriculture and Natural Resources University of Khuzestan, Mollasani, IranAgricultural Research Education and Extension Organization (AREEO) Karaj, Iran The envelope glycoprotein (protein G) of bovine ephemeral fever virus (BEFV) has been identified as a plausible vaccine candidate against the BEF disease. In the present study, G1 epitope of the G gly-coprotein gene was cloned in an eukaryotic expression vector, pcDNA3.1(+), under the control of the human cytomegalovirus (CMV) promoter. The pcDNA3.1-G1 construct was transfected into human embryonic kidney 293 (HEK 293) cell line and the expression efficiency was verified by immunofluorescence staining of transfected cells and Western blot analysis. The results indicated that G1 protein was expressed by the recombinant pcDNA3.1-G1 construct in the transfected cells. The recombinant plasmid constructed in this study can be used as a DNA vaccine to evaluate its potential for immunogenicity and protection against BEF virus in animal models.http://tru.uni-sz.bg/bjvm/BJVM%20June%202018%20p.176-185.pdfbovine ephemeral fever virusDNA vaccineeukaryotic expressionHEK 293 cell linerecombinant G1 protein |
| spellingShingle | R. Pasandideh M.T. Beigi Nassiri M.R.Seyfi Abad Shapouri J. Fayazi H. Roshanfekr M. Lotfi Expression of the G1 epitope of bovine ephemeral fever virus G glycoprotein in eukaryotic cells Bulgarian Journal of Veterinary Medicine bovine ephemeral fever virus DNA vaccine eukaryotic expression HEK 293 cell line recombinant G1 protein |
| title | Expression of the G1 epitope of bovine ephemeral fever virus G glycoprotein in eukaryotic cells |
| title_full | Expression of the G1 epitope of bovine ephemeral fever virus G glycoprotein in eukaryotic cells |
| title_fullStr | Expression of the G1 epitope of bovine ephemeral fever virus G glycoprotein in eukaryotic cells |
| title_full_unstemmed | Expression of the G1 epitope of bovine ephemeral fever virus G glycoprotein in eukaryotic cells |
| title_short | Expression of the G1 epitope of bovine ephemeral fever virus G glycoprotein in eukaryotic cells |
| title_sort | expression of the g1 epitope of bovine ephemeral fever virus g glycoprotein in eukaryotic cells |
| topic | bovine ephemeral fever virus DNA vaccine eukaryotic expression HEK 293 cell line recombinant G1 protein |
| url | http://tru.uni-sz.bg/bjvm/BJVM%20June%202018%20p.176-185.pdf |
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