Purification and Properties of an Insecticidal Metalloprotease Produced by Photorhabdus luminescens Strain 0805-P5G, the Entomopathogenic Nematode Symbiont
A total of 13 Photorhabdus luminescens strains were screened for proteolytic activity. The P. luminescens strain 0805-P5G had the highest activity on both skim milk and gelatin plates. The protease was purified to electrophoretical homogeneity by using a two-step column chromatographic procedure. It...
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MDPI AG
2012-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/14/1/308 |
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| author | Feng-Chia Hsieh Menghsiao Meng Suey-Sheng Kao Hebron C. Chang Li-Ching Wu Yu-Tzu Chang Chienyan Hsieh |
| author_facet | Feng-Chia Hsieh Menghsiao Meng Suey-Sheng Kao Hebron C. Chang Li-Ching Wu Yu-Tzu Chang Chienyan Hsieh |
| author_sort | Feng-Chia Hsieh |
| collection | DOAJ |
| description | A total of 13 Photorhabdus luminescens strains were screened for proteolytic activity. The P. luminescens strain 0805-P5G had the highest activity on both skim milk and gelatin plates. The protease was purified to electrophoretical homogeneity by using a two-step column chromatographic procedure. It had a molecular weight of 51.8 kDa, as determined by MALDI-TOF mass spectrometry. The optimum pH, temperature, as well as pH and thermal stabilities were 8, 60 °C, 5–10, and 14–60 °C, respectively. It was completely inhibited by EDTA and 1,10-phenanthroline. Bioassay of the purified protease against Galleria mellonella by injection showed high insecticidal activity. The protease also showed high oral toxicity to the diamondback moth (Plutella xylostella) of a Taiwan field-collected strain, but low toxicity to an American strain. To our knowledge, this is the first report to demonstrate that the purified protease of P. luminescens has direct toxicity to P. xylostella and biopesticide potentiality. |
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| id | doaj.art-328abab2c503482087d552765ca3397c |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T07:55:17Z |
| publishDate | 2012-12-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-328abab2c503482087d552765ca3397c2022-12-22T03:41:29ZengMDPI AGInternational Journal of Molecular Sciences1422-00672012-12-0114130832110.3390/ijms14010308Purification and Properties of an Insecticidal Metalloprotease Produced by Photorhabdus luminescens Strain 0805-P5G, the Entomopathogenic Nematode SymbiontFeng-Chia HsiehMenghsiao MengSuey-Sheng KaoHebron C. ChangLi-Ching WuYu-Tzu ChangChienyan HsiehA total of 13 Photorhabdus luminescens strains were screened for proteolytic activity. The P. luminescens strain 0805-P5G had the highest activity on both skim milk and gelatin plates. The protease was purified to electrophoretical homogeneity by using a two-step column chromatographic procedure. It had a molecular weight of 51.8 kDa, as determined by MALDI-TOF mass spectrometry. The optimum pH, temperature, as well as pH and thermal stabilities were 8, 60 °C, 5–10, and 14–60 °C, respectively. It was completely inhibited by EDTA and 1,10-phenanthroline. Bioassay of the purified protease against Galleria mellonella by injection showed high insecticidal activity. The protease also showed high oral toxicity to the diamondback moth (Plutella xylostella) of a Taiwan field-collected strain, but low toxicity to an American strain. To our knowledge, this is the first report to demonstrate that the purified protease of P. luminescens has direct toxicity to P. xylostella and biopesticide potentiality.http://www.mdpi.com/1422-0067/14/1/308proteasePhotorhabdus luminescensPlutella xylostellainsecticidal activity |
| spellingShingle | Feng-Chia Hsieh Menghsiao Meng Suey-Sheng Kao Hebron C. Chang Li-Ching Wu Yu-Tzu Chang Chienyan Hsieh Purification and Properties of an Insecticidal Metalloprotease Produced by Photorhabdus luminescens Strain 0805-P5G, the Entomopathogenic Nematode Symbiont International Journal of Molecular Sciences protease Photorhabdus luminescens Plutella xylostella insecticidal activity |
| title | Purification and Properties of an Insecticidal Metalloprotease Produced by Photorhabdus luminescens Strain 0805-P5G, the Entomopathogenic Nematode Symbiont |
| title_full | Purification and Properties of an Insecticidal Metalloprotease Produced by Photorhabdus luminescens Strain 0805-P5G, the Entomopathogenic Nematode Symbiont |
| title_fullStr | Purification and Properties of an Insecticidal Metalloprotease Produced by Photorhabdus luminescens Strain 0805-P5G, the Entomopathogenic Nematode Symbiont |
| title_full_unstemmed | Purification and Properties of an Insecticidal Metalloprotease Produced by Photorhabdus luminescens Strain 0805-P5G, the Entomopathogenic Nematode Symbiont |
| title_short | Purification and Properties of an Insecticidal Metalloprotease Produced by Photorhabdus luminescens Strain 0805-P5G, the Entomopathogenic Nematode Symbiont |
| title_sort | purification and properties of an insecticidal metalloprotease produced by photorhabdus luminescens strain 0805 p5g the entomopathogenic nematode symbiont |
| topic | protease Photorhabdus luminescens Plutella xylostella insecticidal activity |
| url | http://www.mdpi.com/1422-0067/14/1/308 |
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