Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae
Aim: Scabies is one of the most important diseases in goats and caused by a complex hypersensitivity process that involves both humoral and cell-mediated immune responses. This phenomenon shows that the variety of Sarcoptes scabiei has different characteristics of specific antigenic protein or diffe...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Veterinary World
2018-06-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.11/June-2018/13.pdf |
| _version_ | 1818622789927567360 |
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| author | Nunuk Dyah Retno Lastuti Wiwik Misaco Yuniarti Poedji Hastutiek Lucia Tri Suwanti Dony Chrismanto |
| author_facet | Nunuk Dyah Retno Lastuti Wiwik Misaco Yuniarti Poedji Hastutiek Lucia Tri Suwanti Dony Chrismanto |
| author_sort | Nunuk Dyah Retno Lastuti |
| collection | DOAJ |
| description | Aim: Scabies is one of the most important diseases in goats and caused by a complex hypersensitivity process that involves both humoral and cell-mediated immune responses. This phenomenon shows that the variety of Sarcoptes scabiei has different characteristics of specific antigenic protein or different immune-dominant. This research aims to detect the humoral and cellular immune response of rabbits which were immunized with the protein of S. scabiei var. caprae.
Materials and Methods: This research was done as follows, identification and collection of Sarcoptes scabiei var caprae from goat infected with scabies, separation of protein antigen from S. scabiei mites with ultrasonic sonicator, measurement of protein content with spectrophotometry, rabbit injection with 500 μg dose of antigen protein which was repeated 5 times (5x booster) every 2 weeks. Measurement of IgG titer using indirect ELISA, whereas to detect the expression of cellular immune response (TLR-9, CD4, and CD8) using Direct Immunofluorescence assay.
Results: Based on the statistical analysis, it showed that there was a significant enhancement (p<0.05) for optical density value or antibody titer and cellular immune response was shown by TLR-9, CD4, and CD8 expression in rabbit T lymphocytes which appear yellow to green fluorescent color using fluorescence microscope. The amount of fluorescence T lymphocytes showed a significant difference (p<0.05) between the control and various boosters.
Conclusion: Antigenic protein of S. scabiei var. caprae contains ligands, which are involved in the pathogen-associated molecular pattern that has an ability to induce humoral and cellular immune response in rabbit. Specifically, that TLR-9 is not only involved in innate immunity but also in adaptive immunity and can be used as alternative adjuvant development research. |
| first_indexed | 2024-12-16T18:30:45Z |
| format | Article |
| id | doaj.art-328ff4d752014e9188626cba929ff668 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-16T18:30:45Z |
| publishDate | 2018-06-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-328ff4d752014e9188626cba929ff6682022-12-21T22:21:18ZengVeterinary WorldVeterinary World0972-89882231-09162018-06-0111681982310.14202/vetworld.2018.819-823Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. capraeNunuk Dyah Retno Lastuti0Wiwik Misaco Yuniarti1Poedji Hastutiek2Lucia Tri Suwanti3Dony Chrismanto4Department of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.Department of Veterinary Clinic, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.Department of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.Department of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.Study Programme of Animal Health, Faculty of Vocation, Universitas Airlangga, Surabaya, Indonesia.Aim: Scabies is one of the most important diseases in goats and caused by a complex hypersensitivity process that involves both humoral and cell-mediated immune responses. This phenomenon shows that the variety of Sarcoptes scabiei has different characteristics of specific antigenic protein or different immune-dominant. This research aims to detect the humoral and cellular immune response of rabbits which were immunized with the protein of S. scabiei var. caprae. Materials and Methods: This research was done as follows, identification and collection of Sarcoptes scabiei var caprae from goat infected with scabies, separation of protein antigen from S. scabiei mites with ultrasonic sonicator, measurement of protein content with spectrophotometry, rabbit injection with 500 μg dose of antigen protein which was repeated 5 times (5x booster) every 2 weeks. Measurement of IgG titer using indirect ELISA, whereas to detect the expression of cellular immune response (TLR-9, CD4, and CD8) using Direct Immunofluorescence assay. Results: Based on the statistical analysis, it showed that there was a significant enhancement (p<0.05) for optical density value or antibody titer and cellular immune response was shown by TLR-9, CD4, and CD8 expression in rabbit T lymphocytes which appear yellow to green fluorescent color using fluorescence microscope. The amount of fluorescence T lymphocytes showed a significant difference (p<0.05) between the control and various boosters. Conclusion: Antigenic protein of S. scabiei var. caprae contains ligands, which are involved in the pathogen-associated molecular pattern that has an ability to induce humoral and cellular immune response in rabbit. Specifically, that TLR-9 is not only involved in innate immunity but also in adaptive immunity and can be used as alternative adjuvant development research.http://www.veterinaryworld.org/Vol.11/June-2018/13.pdfantigenic proteinCD4CD8immunoglobulin GSarcoptes scabiei var. capraetoll-like receptor-9 |
| spellingShingle | Nunuk Dyah Retno Lastuti Wiwik Misaco Yuniarti Poedji Hastutiek Lucia Tri Suwanti Dony Chrismanto Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae Veterinary World antigenic protein CD4 CD8 immunoglobulin G Sarcoptes scabiei var. caprae toll-like receptor-9 |
| title | Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae |
| title_full | Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae |
| title_fullStr | Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae |
| title_full_unstemmed | Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae |
| title_short | Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae |
| title_sort | humoral and cellular immune response induced by antigenic protein of sarcoptes scabiei var caprae |
| topic | antigenic protein CD4 CD8 immunoglobulin G Sarcoptes scabiei var. caprae toll-like receptor-9 |
| url | http://www.veterinaryworld.org/Vol.11/June-2018/13.pdf |
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