Adaptation of <i>Brucella melitensis</i> Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method
Brucellosis, mainly caused by <i>Brucella</i> (B.) <i>melitensis</i>, is associated with a risk of chronification and relapses. Antimicrobial susceptibility testing (AST) standards for <i>B. melitensis</i> are not available, and the agent is not yet listed in the...
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MDPI AG
2022-07-01
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| author | Alina Tscherne Enrico Mantel Talar Boskani Sylwia Budniak Mandy Elschner Antonio Fasanella Siri L. Feruglio Domenico Galante Christian G. Giske Roland Grunow Judit Henczko Christin Hinz Wojciech Iwaniak Daniela Jacob Agnieszka Kedrak-Jablonska Veronica K. Jensen Tone B. Johansen Gunnar Kahlmeter Viviana Manzulli Erika Matuschek Falk Melzer Maria S. Nuncio Joseph Papaparaskevas Ana Pelerito Margrete Solheim Susanne Thomann Athanasios Tsakris Tara Wahab Marcin Weiner Lothar Zoeller Sabine Zange |
| author_facet | Alina Tscherne Enrico Mantel Talar Boskani Sylwia Budniak Mandy Elschner Antonio Fasanella Siri L. Feruglio Domenico Galante Christian G. Giske Roland Grunow Judit Henczko Christin Hinz Wojciech Iwaniak Daniela Jacob Agnieszka Kedrak-Jablonska Veronica K. Jensen Tone B. Johansen Gunnar Kahlmeter Viviana Manzulli Erika Matuschek Falk Melzer Maria S. Nuncio Joseph Papaparaskevas Ana Pelerito Margrete Solheim Susanne Thomann Athanasios Tsakris Tara Wahab Marcin Weiner Lothar Zoeller Sabine Zange |
| author_sort | Alina Tscherne |
| collection | DOAJ |
| description | Brucellosis, mainly caused by <i>Brucella</i> (B.) <i>melitensis</i>, is associated with a risk of chronification and relapses. Antimicrobial susceptibility testing (AST) standards for <i>B. melitensis</i> are not available, and the agent is not yet listed in the EUCAST breakpoint tables. CLSI recommendations for <i>B. melitensis</i> exist, but they do not fulfill the requirements of the ISO 20776 standard regarding the culture medium and the incubation conditions. Under the third EU Health Programme, laboratories specializing in the diagnostics of highly pathogenic bacteria in their respective countries formed a working group within a Joint Action aiming to develop a suitable method for the AST of <i>B. melitensis</i>. Under the supervision of EUCAST representatives, this working group adapted the CLSI M45 document to the ISO 20776 standard after testing and validation. These adaptations included the comparison of various culture media, culture conditions and AST methods. A Standard Operation Procedure was derived and an interlaboratory validation was performed in order to evaluate the method. The results showed pros and cons for both of the two methods but also indicate that it is not necessary to abandon Mueller–Hinton without additives for the AST of <i>B. melitensis</i>. |
| first_indexed | 2024-03-09T06:09:12Z |
| format | Article |
| id | doaj.art-3292b027dd2240268b29626663aaa004 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T06:09:12Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-3292b027dd2240268b29626663aaa0042023-12-03T11:59:37ZengMDPI AGMicroorganisms2076-26072022-07-01107147010.3390/microorganisms10071470Adaptation of <i>Brucella melitensis</i> Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the MethodAlina Tscherne0Enrico Mantel1Talar Boskani2Sylwia Budniak3Mandy Elschner4Antonio Fasanella5Siri L. Feruglio6Domenico Galante7Christian G. Giske8Roland Grunow9Judit Henczko10Christin Hinz11Wojciech Iwaniak12Daniela Jacob13Agnieszka Kedrak-Jablonska14Veronica K. Jensen15Tone B. Johansen16Gunnar Kahlmeter17Viviana Manzulli18Erika Matuschek19Falk Melzer20Maria S. Nuncio21Joseph Papaparaskevas22Ana Pelerito23Margrete Solheim24Susanne Thomann25Athanasios Tsakris26Tara Wahab27Marcin Weiner28Lothar Zoeller29Sabine Zange30Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, GermanyBundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, GermanyPublic Health Agency of Sweden, Nobels väg 18, 171 82 Solna, SwedenNational Veterinary Research Institute, 57 Partyzantów Avenue, 24-100 Pulawy, PolandFriedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Strasse 96a, 07743 Jena, GermanyIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 20 Manfredonia Street, 72121 Foggia, ItalyNorwegian Institute of Public Health, Lovisenberggata 8, 0456 Oslo, NorwayIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 20 Manfredonia Street, 72121 Foggia, ItalyDivision of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Alfred Nobels Allé 8, Huddinge, 141 52 Stockholm, SwedenHighly Pathogenic Microorganisms, Centre for Biological Threats and Special Pathogens, Division 2 (ZBS 2), Robert Koch Institute, Seestrasse 10, 13353 Berlin, GermanyNational Public Health Center, Albert Flórián út 2-6, 1097 Budapest, HungaryBundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, GermanyCEDROB S.A., Ujazdowek 2A, 06-400 Ciechanow, PolandHighly Pathogenic Microorganisms, Centre for Biological Threats and Special Pathogens, Division 2 (ZBS 2), Robert Koch Institute, Seestrasse 10, 13353 Berlin, GermanyNational Veterinary Research Institute, 57 Partyzantów Avenue, 24-100 Pulawy, PolandNorwegian Institute of Public Health, Lovisenberggata 8, 0456 Oslo, NorwayNorwegian Institute of Public Health, Lovisenberggata 8, 0456 Oslo, NorwayEUCAST Development Laboratory (EDL) for Bacteria, 351 85 Växjö, SwedenIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 20 Manfredonia Street, 72121 Foggia, ItalyEUCAST Development Laboratory (EDL) for Bacteria, 351 85 Växjö, SwedenFriedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Strasse 96a, 07743 Jena, GermanyNational Institute of Health, Av. Padre Cruz, 1649-016 Lisbon, PortugalMicrobiology Department, Medical School, National and Kapodistrian University of Athens, Mikras Asias 75, 11527 Athens, GreeceNational Institute of Health, Av. Padre Cruz, 1649-016 Lisbon, PortugalNorwegian Institute of Public Health, Lovisenberggata 8, 0456 Oslo, NorwayLaboratory of Spiez, Austrasse, 3700 Spiez, SwitzerlandMicrobiology Department, Medical School, National and Kapodistrian University of Athens, Mikras Asias 75, 11527 Athens, GreecePublic Health Agency of Sweden, Nobels väg 18, 171 82 Solna, SwedenNational Veterinary Research Institute, 57 Partyzantów Avenue, 24-100 Pulawy, PolandBundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, GermanyBundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, GermanyBrucellosis, mainly caused by <i>Brucella</i> (B.) <i>melitensis</i>, is associated with a risk of chronification and relapses. Antimicrobial susceptibility testing (AST) standards for <i>B. melitensis</i> are not available, and the agent is not yet listed in the EUCAST breakpoint tables. CLSI recommendations for <i>B. melitensis</i> exist, but they do not fulfill the requirements of the ISO 20776 standard regarding the culture medium and the incubation conditions. Under the third EU Health Programme, laboratories specializing in the diagnostics of highly pathogenic bacteria in their respective countries formed a working group within a Joint Action aiming to develop a suitable method for the AST of <i>B. melitensis</i>. Under the supervision of EUCAST representatives, this working group adapted the CLSI M45 document to the ISO 20776 standard after testing and validation. These adaptations included the comparison of various culture media, culture conditions and AST methods. A Standard Operation Procedure was derived and an interlaboratory validation was performed in order to evaluate the method. The results showed pros and cons for both of the two methods but also indicate that it is not necessary to abandon Mueller–Hinton without additives for the AST of <i>B. melitensis</i>.https://www.mdpi.com/2076-2607/10/7/1470<i>Brucella melitensis</i>antimicrobial susceptibility testinginterlaboratory validationISO 20776 standardCLSI |
| spellingShingle | Alina Tscherne Enrico Mantel Talar Boskani Sylwia Budniak Mandy Elschner Antonio Fasanella Siri L. Feruglio Domenico Galante Christian G. Giske Roland Grunow Judit Henczko Christin Hinz Wojciech Iwaniak Daniela Jacob Agnieszka Kedrak-Jablonska Veronica K. Jensen Tone B. Johansen Gunnar Kahlmeter Viviana Manzulli Erika Matuschek Falk Melzer Maria S. Nuncio Joseph Papaparaskevas Ana Pelerito Margrete Solheim Susanne Thomann Athanasios Tsakris Tara Wahab Marcin Weiner Lothar Zoeller Sabine Zange Adaptation of <i>Brucella melitensis</i> Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method Microorganisms <i>Brucella melitensis</i> antimicrobial susceptibility testing interlaboratory validation ISO 20776 standard CLSI |
| title | Adaptation of <i>Brucella melitensis</i> Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method |
| title_full | Adaptation of <i>Brucella melitensis</i> Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method |
| title_fullStr | Adaptation of <i>Brucella melitensis</i> Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method |
| title_full_unstemmed | Adaptation of <i>Brucella melitensis</i> Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method |
| title_short | Adaptation of <i>Brucella melitensis</i> Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method |
| title_sort | adaptation of i brucella melitensis i antimicrobial susceptibility testing to the iso 20776 standard and validation of the method |
| topic | <i>Brucella melitensis</i> antimicrobial susceptibility testing interlaboratory validation ISO 20776 standard CLSI |
| url | https://www.mdpi.com/2076-2607/10/7/1470 |
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