Molecular Identification of <i>Zantedeschia</i> Culture with Determination of Its Morphometric and Metabolic Activities for Mediterranean Acclimatization
Calla lily (<i>Zantedeschia albomaculata</i> (Hook.) Baill.) is an herbaceous or semi-evergreen perennial grown from rhizomes. It is commonly named “Spotted Arum”. Ribosomal RNAs (rRNAs) are found in all known organisms and are known for being functionally equivalent in all of them. A co...
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MDPI AG
2022-09-01
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| author | Eman Tawfik Mohamed Fathy Ahmed Doha A. Albalawi Bandar S. Aljuaid Doaa Bahaa Eldin Darwish Samy F. Mahmoud Karim M. Hassan Mohamed F. M. Ibrahim Ashraf Bakry Abdel Razik |
| author_facet | Eman Tawfik Mohamed Fathy Ahmed Doha A. Albalawi Bandar S. Aljuaid Doaa Bahaa Eldin Darwish Samy F. Mahmoud Karim M. Hassan Mohamed F. M. Ibrahim Ashraf Bakry Abdel Razik |
| author_sort | Eman Tawfik |
| collection | DOAJ |
| description | Calla lily (<i>Zantedeschia albomaculata</i> (Hook.) Baill.) is an herbaceous or semi-evergreen perennial grown from rhizomes. It is commonly named “Spotted Arum”. Ribosomal RNAs (rRNAs) are found in all known organisms and are known for being functionally equivalent in all of them. A completely new in vitro culture protocol was applied to <i>Z. albomaculata</i> with two hormones, 6-Benzylaminopurine (BAP) and kinetin, to obtain full growth and multiplication. Due to their highly conserved sequences, the analysis of small-subunit rRNAs (16S–18S rRNAs) can provide precise statistical evaluation of a wide variety of phylogenetic connections. As a result, the plant’s 18S rRNA gene allowed for identification and partial sequencing. Also, the traditional floral method and the novel application technique for identification were applied to <i>Z. albomaculata</i>. In this paper we systemically describe the structural strategies of the plant’s adaptation to the surroundings at the morphological, physiological, and anatomical scale. Most the essential oils and fatty acids found in <i>Z. albomaculata</i> are omega fatty acids, octadecenoic acid, linoleic acid, and palmitic acid. All these fatty acids have industrial, medicinal, and pharmaceutical applications. The significant findings are the spadix sheathing leaves, and the precipitation of raphides calcium oxalate. The mitotic index showing the division activity was recorded, and it was 17.4%. The antimicrobial activity of <i>Z. albomaculata</i> ethanol extract was performed via the well diffusion method. This extract has shown high activity against <i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i>, compared to its lower activity against <i>Bacillus cereus</i>. By defining these characteristics and in vitro culture conditions, we will be able to acclimatize the plant in greenhouses, and then transfer it to the open field. The findings of this work identified the general characteristics of <i>Zantedeschia albomaculata</i> as an ornamental and medicinal plant in order to acclimatize this plant for cultivation in the Mediterranean climate. |
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| spelling | doaj.art-3295479ec99d4226bef65b36e6c0a8bd2023-11-23T13:56:33ZengMDPI AGPlants2223-77472022-09-011117231110.3390/plants11172311Molecular Identification of <i>Zantedeschia</i> Culture with Determination of Its Morphometric and Metabolic Activities for Mediterranean AcclimatizationEman Tawfik0Mohamed Fathy Ahmed1Doha A. Albalawi2Bandar S. Aljuaid3Doaa Bahaa Eldin Darwish4Samy F. Mahmoud5Karim M. Hassan6Mohamed F. M. Ibrahim7Ashraf Bakry Abdel Razik8Botany and Microbiology Department, Faculty of Science, Helwan University, Cairo 11566, EgyptHorticulture Department, Faculty of Agriculture, Ain Shams University, Cairo 11566, EgyptDepartment of Biology, Faculty of Science, University of Tabuk, Tabuk 71491, Saudi ArabiaDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaBotany Department, Faculty of Science, Mansoura University, Mansoura 35511, EgyptDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaHorticulture Department, Faculty of Agriculture, Ain Shams University, Cairo 11566, EgyptDepartment of Agricultural Botany, Faculty of Agriculture, Ain Shams University, Cairo 11566, EgyptGenetics Department, Faculty of Agriculture, Ain Shams University, Cairo 11566, EgyptCalla lily (<i>Zantedeschia albomaculata</i> (Hook.) Baill.) is an herbaceous or semi-evergreen perennial grown from rhizomes. It is commonly named “Spotted Arum”. Ribosomal RNAs (rRNAs) are found in all known organisms and are known for being functionally equivalent in all of them. A completely new in vitro culture protocol was applied to <i>Z. albomaculata</i> with two hormones, 6-Benzylaminopurine (BAP) and kinetin, to obtain full growth and multiplication. Due to their highly conserved sequences, the analysis of small-subunit rRNAs (16S–18S rRNAs) can provide precise statistical evaluation of a wide variety of phylogenetic connections. As a result, the plant’s 18S rRNA gene allowed for identification and partial sequencing. Also, the traditional floral method and the novel application technique for identification were applied to <i>Z. albomaculata</i>. In this paper we systemically describe the structural strategies of the plant’s adaptation to the surroundings at the morphological, physiological, and anatomical scale. Most the essential oils and fatty acids found in <i>Z. albomaculata</i> are omega fatty acids, octadecenoic acid, linoleic acid, and palmitic acid. All these fatty acids have industrial, medicinal, and pharmaceutical applications. The significant findings are the spadix sheathing leaves, and the precipitation of raphides calcium oxalate. The mitotic index showing the division activity was recorded, and it was 17.4%. The antimicrobial activity of <i>Z. albomaculata</i> ethanol extract was performed via the well diffusion method. This extract has shown high activity against <i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i>, compared to its lower activity against <i>Bacillus cereus</i>. By defining these characteristics and in vitro culture conditions, we will be able to acclimatize the plant in greenhouses, and then transfer it to the open field. The findings of this work identified the general characteristics of <i>Zantedeschia albomaculata</i> as an ornamental and medicinal plant in order to acclimatize this plant for cultivation in the Mediterranean climate.https://www.mdpi.com/2223-7747/11/17/2311<i>Zantedeschia albomaculata</i>in vitro culturepigmentationmorphological descriptionanatomyphenolics |
| spellingShingle | Eman Tawfik Mohamed Fathy Ahmed Doha A. Albalawi Bandar S. Aljuaid Doaa Bahaa Eldin Darwish Samy F. Mahmoud Karim M. Hassan Mohamed F. M. Ibrahim Ashraf Bakry Abdel Razik Molecular Identification of <i>Zantedeschia</i> Culture with Determination of Its Morphometric and Metabolic Activities for Mediterranean Acclimatization Plants <i>Zantedeschia albomaculata</i> in vitro culture pigmentation morphological description anatomy phenolics |
| title | Molecular Identification of <i>Zantedeschia</i> Culture with Determination of Its Morphometric and Metabolic Activities for Mediterranean Acclimatization |
| title_full | Molecular Identification of <i>Zantedeschia</i> Culture with Determination of Its Morphometric and Metabolic Activities for Mediterranean Acclimatization |
| title_fullStr | Molecular Identification of <i>Zantedeschia</i> Culture with Determination of Its Morphometric and Metabolic Activities for Mediterranean Acclimatization |
| title_full_unstemmed | Molecular Identification of <i>Zantedeschia</i> Culture with Determination of Its Morphometric and Metabolic Activities for Mediterranean Acclimatization |
| title_short | Molecular Identification of <i>Zantedeschia</i> Culture with Determination of Its Morphometric and Metabolic Activities for Mediterranean Acclimatization |
| title_sort | molecular identification of i zantedeschia i culture with determination of its morphometric and metabolic activities for mediterranean acclimatization |
| topic | <i>Zantedeschia albomaculata</i> in vitro culture pigmentation morphological description anatomy phenolics |
| url | https://www.mdpi.com/2223-7747/11/17/2311 |
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