Analysis of Compound Stained Cervical Cell Images Using Multi-Spectral Imaging
Multi-spectral imaging technique plays an important role in real-world applications such as medicine and medical detections. This paper proposes a cervical cancer cell screening method to simultaneously adopt TBS classification and DNA quantitative analysis for a single cell smear. Through using com...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/12/5628 |
| _version_ | 1797529691300560896 |
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| author | Run Fang Libo Zeng Fan Yi |
| author_facet | Run Fang Libo Zeng Fan Yi |
| author_sort | Run Fang |
| collection | DOAJ |
| description | Multi-spectral imaging technique plays an important role in real-world applications such as medicine and medical detections. This paper proposes a cervical cancer cell screening method to simultaneously adopt TBS classification and DNA quantitative analysis for a single cell smear. Through using compound staining on a smear, the cytoplasm is stained by Papanicolauo and the nucleus is stained by Feulgen. The main evaluation parameter is the DNA content of the nucleus, not the subjective description of cell morphology, which is more objective than the TBS classification method and reduces the chances of missing a diagnosis due to subjective factors. Each nucleus has its own DI value and color image of the whole cell, which is convenient for doctors as it allows them to review and confirm the morphology of cells with a nucleus DI of over 2.5. Mouse liver smears and cervical cases are utilized as the measuring specimens to evaluate the performance of the microscope multi-spectral imaging system; illustrative results demonstrate that the proposed system qualifies, with high accuracy and reliability, and further presents wide application prospects in the early diagnosis of cervical cancer. |
| first_indexed | 2024-03-10T10:18:18Z |
| format | Article |
| id | doaj.art-329599702a0940049e9486b42ab11eca |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T10:18:18Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-329599702a0940049e9486b42ab11eca2023-11-22T00:38:49ZengMDPI AGApplied Sciences2076-34172021-06-011112562810.3390/app11125628Analysis of Compound Stained Cervical Cell Images Using Multi-Spectral ImagingRun Fang0Libo Zeng1Fan Yi2Electronic Information School, Wuhan University, Wuhan 430072, ChinaElectronic Information School, Wuhan University, Wuhan 430072, ChinaElectronic Information School, Wuhan University, Wuhan 430072, ChinaMulti-spectral imaging technique plays an important role in real-world applications such as medicine and medical detections. This paper proposes a cervical cancer cell screening method to simultaneously adopt TBS classification and DNA quantitative analysis for a single cell smear. Through using compound staining on a smear, the cytoplasm is stained by Papanicolauo and the nucleus is stained by Feulgen. The main evaluation parameter is the DNA content of the nucleus, not the subjective description of cell morphology, which is more objective than the TBS classification method and reduces the chances of missing a diagnosis due to subjective factors. Each nucleus has its own DI value and color image of the whole cell, which is convenient for doctors as it allows them to review and confirm the morphology of cells with a nucleus DI of over 2.5. Mouse liver smears and cervical cases are utilized as the measuring specimens to evaluate the performance of the microscope multi-spectral imaging system; illustrative results demonstrate that the proposed system qualifies, with high accuracy and reliability, and further presents wide application prospects in the early diagnosis of cervical cancer.https://www.mdpi.com/2076-3417/11/12/5628multi-spectral imagingcomposite light sourceabsorbance unmixingpseudo-color image |
| spellingShingle | Run Fang Libo Zeng Fan Yi Analysis of Compound Stained Cervical Cell Images Using Multi-Spectral Imaging Applied Sciences multi-spectral imaging composite light source absorbance unmixing pseudo-color image |
| title | Analysis of Compound Stained Cervical Cell Images Using Multi-Spectral Imaging |
| title_full | Analysis of Compound Stained Cervical Cell Images Using Multi-Spectral Imaging |
| title_fullStr | Analysis of Compound Stained Cervical Cell Images Using Multi-Spectral Imaging |
| title_full_unstemmed | Analysis of Compound Stained Cervical Cell Images Using Multi-Spectral Imaging |
| title_short | Analysis of Compound Stained Cervical Cell Images Using Multi-Spectral Imaging |
| title_sort | analysis of compound stained cervical cell images using multi spectral imaging |
| topic | multi-spectral imaging composite light source absorbance unmixing pseudo-color image |
| url | https://www.mdpi.com/2076-3417/11/12/5628 |
| work_keys_str_mv | AT runfang analysisofcompoundstainedcervicalcellimagesusingmultispectralimaging AT libozeng analysisofcompoundstainedcervicalcellimagesusingmultispectralimaging AT fanyi analysisofcompoundstainedcervicalcellimagesusingmultispectralimaging |