Effects of Dermatan Sulfate from Marine Invertebrate <i>Styela plicata</i> in the Wound Healing Pathway: A Natural Resource Applied to Regenerative Therapy
Acute and chronic dermatological injuries need rapid tissue repair due to the susceptibility to infections. To effectively promote cutaneous wound recovery, it is essential to develop safe, low-cost, and affordable regenerative tools. Therefore, we aimed to identify the biological mechanisms involve...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/11/676 |
| _version_ | 1797467502640365568 |
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| author | Vanessa S. Rizzo-Valente Maria A. Fusco Renata M. M. L. Cruz Rachel A. Santos Lucas S. Silva Roberta C. Escaleira Daniel F. Schulz Shana P. C. Barroso Bruno L. Miranda Daniela Z. Santos Marcelo L. Gregório Rodrigo J. A. Guerra Mauro S. G. Pavão |
| author_facet | Vanessa S. Rizzo-Valente Maria A. Fusco Renata M. M. L. Cruz Rachel A. Santos Lucas S. Silva Roberta C. Escaleira Daniel F. Schulz Shana P. C. Barroso Bruno L. Miranda Daniela Z. Santos Marcelo L. Gregório Rodrigo J. A. Guerra Mauro S. G. Pavão |
| author_sort | Vanessa S. Rizzo-Valente |
| collection | DOAJ |
| description | Acute and chronic dermatological injuries need rapid tissue repair due to the susceptibility to infections. To effectively promote cutaneous wound recovery, it is essential to develop safe, low-cost, and affordable regenerative tools. Therefore, we aimed to identify the biological mechanisms involved in the wound healing properties of the glycosaminoglycan dermatan sulfate (DS), obtained from ascidian <i>Styela plicata</i>, a marine invertebrate, which in preliminary work from our group showed no toxicity and promoted a remarkable fibroblast proliferation and migration. In this study, 2,4-DS (50 µg/mL)-treated and control groups had the relative gene expression of 84 genes participating in the healing pathway evaluated. The results showed that 57% of the genes were overexpressed during treatment, 16% were underexpressed, and 9.52% were not detected. In silico analysis of metabolic interactions exhibited overexpression of genes related to: extracellular matrix organization, hemostasis, secretion of inflammatory mediators, and regulation of insulin-like growth factor transport and uptake. Furthermore, in C57BL/6 mice subjected to experimental wounds treated with 0.25% 2,4-DS, the histological parameters demonstrated a great capacity for vascular recovery. Additionally, this study confirmed that DS is a potent inducer of wound-healing cellular pathways and a promoter of neovascularization, being a natural ally in the tissue regeneration strategy. |
| first_indexed | 2024-03-09T18:53:34Z |
| format | Article |
| id | doaj.art-3296ef82db0f4003ae4f98c878df265e |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T18:53:34Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-3296ef82db0f4003ae4f98c878df265e2023-11-24T05:34:17ZengMDPI AGMarine Drugs1660-33972022-10-01201167610.3390/md20110676Effects of Dermatan Sulfate from Marine Invertebrate <i>Styela plicata</i> in the Wound Healing Pathway: A Natural Resource Applied to Regenerative TherapyVanessa S. Rizzo-Valente0Maria A. Fusco1Renata M. M. L. Cruz2Rachel A. Santos3Lucas S. Silva4Roberta C. Escaleira5Daniel F. Schulz6Shana P. C. Barroso7Bruno L. Miranda8Daniela Z. Santos9Marcelo L. Gregório10Rodrigo J. A. Guerra11Mauro S. G. Pavão12Biomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, BrazilBiomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, BrazilBiomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, BrazilBiomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, BrazilBiomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, BrazilBiomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, BrazilBiomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, BrazilBiomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, BrazilBiomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, BrazilBiomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, BrazilBiomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, BrazilBiomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, BrazilLaboratory of Biochemistry and Cell Biology of Glycoconjugates, Clementino Fraga Filho University Hospital and Institute of Medical Biochemistry Leopoldo De Meis, Federal University of Rio de Janeiro, Rio de Janeiro 21941-913, BrazilAcute and chronic dermatological injuries need rapid tissue repair due to the susceptibility to infections. To effectively promote cutaneous wound recovery, it is essential to develop safe, low-cost, and affordable regenerative tools. Therefore, we aimed to identify the biological mechanisms involved in the wound healing properties of the glycosaminoglycan dermatan sulfate (DS), obtained from ascidian <i>Styela plicata</i>, a marine invertebrate, which in preliminary work from our group showed no toxicity and promoted a remarkable fibroblast proliferation and migration. In this study, 2,4-DS (50 µg/mL)-treated and control groups had the relative gene expression of 84 genes participating in the healing pathway evaluated. The results showed that 57% of the genes were overexpressed during treatment, 16% were underexpressed, and 9.52% were not detected. In silico analysis of metabolic interactions exhibited overexpression of genes related to: extracellular matrix organization, hemostasis, secretion of inflammatory mediators, and regulation of insulin-like growth factor transport and uptake. Furthermore, in C57BL/6 mice subjected to experimental wounds treated with 0.25% 2,4-DS, the histological parameters demonstrated a great capacity for vascular recovery. Additionally, this study confirmed that DS is a potent inducer of wound-healing cellular pathways and a promoter of neovascularization, being a natural ally in the tissue regeneration strategy.https://www.mdpi.com/1660-3397/20/11/676ascidiandermatan sulfatefibroblast<i>Styela plicata</i>wound healing |
| spellingShingle | Vanessa S. Rizzo-Valente Maria A. Fusco Renata M. M. L. Cruz Rachel A. Santos Lucas S. Silva Roberta C. Escaleira Daniel F. Schulz Shana P. C. Barroso Bruno L. Miranda Daniela Z. Santos Marcelo L. Gregório Rodrigo J. A. Guerra Mauro S. G. Pavão Effects of Dermatan Sulfate from Marine Invertebrate <i>Styela plicata</i> in the Wound Healing Pathway: A Natural Resource Applied to Regenerative Therapy Marine Drugs ascidian dermatan sulfate fibroblast <i>Styela plicata</i> wound healing |
| title | Effects of Dermatan Sulfate from Marine Invertebrate <i>Styela plicata</i> in the Wound Healing Pathway: A Natural Resource Applied to Regenerative Therapy |
| title_full | Effects of Dermatan Sulfate from Marine Invertebrate <i>Styela plicata</i> in the Wound Healing Pathway: A Natural Resource Applied to Regenerative Therapy |
| title_fullStr | Effects of Dermatan Sulfate from Marine Invertebrate <i>Styela plicata</i> in the Wound Healing Pathway: A Natural Resource Applied to Regenerative Therapy |
| title_full_unstemmed | Effects of Dermatan Sulfate from Marine Invertebrate <i>Styela plicata</i> in the Wound Healing Pathway: A Natural Resource Applied to Regenerative Therapy |
| title_short | Effects of Dermatan Sulfate from Marine Invertebrate <i>Styela plicata</i> in the Wound Healing Pathway: A Natural Resource Applied to Regenerative Therapy |
| title_sort | effects of dermatan sulfate from marine invertebrate i styela plicata i in the wound healing pathway a natural resource applied to regenerative therapy |
| topic | ascidian dermatan sulfate fibroblast <i>Styela plicata</i> wound healing |
| url | https://www.mdpi.com/1660-3397/20/11/676 |
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