Telomerase RNA component lncRNA as potential diagnostic biomarker promotes CRC cellular migration and apoptosis evasion via modulation of β-catenin protein level
Aim: Long non-coding RNA (LncRNA) telomerase RNA component (TERC) has telomerase-dependent and independent activity in numerous cancer types. The present study purposes to demonstrate the role of lncRNA TERC as a diagnostic serum biomarker in colorectal cancer (CRC) patients and the molecular mechan...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-09-01
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| Series: | Non-coding RNA Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468054023000203 |
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| author | Mohamed Bakr Mai A. Abd-Elmawla Hanan Elimam Hebatallah Gamal El-Din Amal Fawzy Ahmed I. Abulsoud Sherine M. Rizk |
| author_facet | Mohamed Bakr Mai A. Abd-Elmawla Hanan Elimam Hebatallah Gamal El-Din Amal Fawzy Ahmed I. Abulsoud Sherine M. Rizk |
| author_sort | Mohamed Bakr |
| collection | DOAJ |
| description | Aim: Long non-coding RNA (LncRNA) telomerase RNA component (TERC) has telomerase-dependent and independent activity in numerous cancer types. The present study purposes to demonstrate the role of lncRNA TERC as a diagnostic serum biomarker in colorectal cancer (CRC) patients and the molecular mechanism of lncRNA TERC in inducing tumor in CRC cell lines. Materials and methods: PCR array was performed to examine lncRNAs dysregulated in CRC. LncRNA TERC expression level was evaluated in 70 CRC patients and 35 control subjects using RT-qPCR. Then transfection was performed to build down-expression models of lncRNA TERC. ROC curve analysis was applied to assess the diagnostic value of serum LncRNA CRC. In addition, RT-qPCR was used to detect expression level of lncRNA TERC and β-catenin mRNA. Moreover, ELISA and Western blot were used to detect the level of β-catenin protein in sera of CRC patients and cell lines. The biological functions such as cell growth and migration of CRC cells were assessed using a wound healing assay. Cell cycle analysis and apoptosis analysis were performed using flow cytometry. Results: The lncRNA TERC is overexpressed in the sera of CRC patients with high diagnostic and stage discrimination accuracy. Furthermore, lncRNA TERC expression was upregulated in CRC cell lines and lncRNA TERC silencing induced cell arrest and apoptosis and inhibited cell migration. Furthermore, inhibition of lncRNA TERC reduces β-catenin protein levels. Conclusion: The lncRNA TERC could be considered as an early stages CRC diagnostic biomarker with a good ability to discriminate between CRC stages. lncRNA TERC induces CRC by promoting cell migration and evading apoptosis by elevating the level of β-catenin protein. |
| first_indexed | 2024-03-13T02:24:52Z |
| format | Article |
| id | doaj.art-32971c03c80f463da5ef881e8d098a04 |
| institution | Directory Open Access Journal |
| issn | 2468-0540 |
| language | English |
| last_indexed | 2024-03-13T02:24:52Z |
| publishDate | 2023-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Non-coding RNA Research |
| spelling | doaj.art-32971c03c80f463da5ef881e8d098a042023-06-30T04:22:31ZengKeAi Communications Co., Ltd.Non-coding RNA Research2468-05402023-09-0183302314Telomerase RNA component lncRNA as potential diagnostic biomarker promotes CRC cellular migration and apoptosis evasion via modulation of β-catenin protein levelMohamed Bakr0Mai A. Abd-Elmawla1Hanan Elimam2Hebatallah Gamal El-Din3Amal Fawzy4Ahmed I. Abulsoud5Sherine M. Rizk6Department of Biochemistry, Faculty of Pharmacy, University of Sadat City, Sadat City, EgyptDepartment of Biochemistry, Faculty of Pharmacy, Cairo University, Cairo, Egypt; Corresponding author.Department of Biochemistry, Faculty of Pharmacy, University of Sadat City, Sadat City, Egypt; Department of Biochemistry, Faculty of Pharmacy, Sinai University, Kantara, EgyptDepartment of Surgical Oncology, National Cancer Institute, Cairo University, Cairo, EgyptDepartment of Clinical and Chemical Pathology, National Cancer Institute, Cairo University, Cairo, EgyptDepartment of Biochemistry, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt; Department of Biochemistry, Faculty of Pharmacy, Heliopolis University, Cairo, EgyptDepartment of Biochemistry, Faculty of Pharmacy, Cairo University, Cairo, EgyptAim: Long non-coding RNA (LncRNA) telomerase RNA component (TERC) has telomerase-dependent and independent activity in numerous cancer types. The present study purposes to demonstrate the role of lncRNA TERC as a diagnostic serum biomarker in colorectal cancer (CRC) patients and the molecular mechanism of lncRNA TERC in inducing tumor in CRC cell lines. Materials and methods: PCR array was performed to examine lncRNAs dysregulated in CRC. LncRNA TERC expression level was evaluated in 70 CRC patients and 35 control subjects using RT-qPCR. Then transfection was performed to build down-expression models of lncRNA TERC. ROC curve analysis was applied to assess the diagnostic value of serum LncRNA CRC. In addition, RT-qPCR was used to detect expression level of lncRNA TERC and β-catenin mRNA. Moreover, ELISA and Western blot were used to detect the level of β-catenin protein in sera of CRC patients and cell lines. The biological functions such as cell growth and migration of CRC cells were assessed using a wound healing assay. Cell cycle analysis and apoptosis analysis were performed using flow cytometry. Results: The lncRNA TERC is overexpressed in the sera of CRC patients with high diagnostic and stage discrimination accuracy. Furthermore, lncRNA TERC expression was upregulated in CRC cell lines and lncRNA TERC silencing induced cell arrest and apoptosis and inhibited cell migration. Furthermore, inhibition of lncRNA TERC reduces β-catenin protein levels. Conclusion: The lncRNA TERC could be considered as an early stages CRC diagnostic biomarker with a good ability to discriminate between CRC stages. lncRNA TERC induces CRC by promoting cell migration and evading apoptosis by elevating the level of β-catenin protein.http://www.sciencedirect.com/science/article/pii/S2468054023000203Colorectal cancerLncRNAsTERCDiagnostic biomarkersApoptosis evadingβ-catenin protein |
| spellingShingle | Mohamed Bakr Mai A. Abd-Elmawla Hanan Elimam Hebatallah Gamal El-Din Amal Fawzy Ahmed I. Abulsoud Sherine M. Rizk Telomerase RNA component lncRNA as potential diagnostic biomarker promotes CRC cellular migration and apoptosis evasion via modulation of β-catenin protein level Non-coding RNA Research Colorectal cancer LncRNAs TERC Diagnostic biomarkers Apoptosis evading β-catenin protein |
| title | Telomerase RNA component lncRNA as potential diagnostic biomarker promotes CRC cellular migration and apoptosis evasion via modulation of β-catenin protein level |
| title_full | Telomerase RNA component lncRNA as potential diagnostic biomarker promotes CRC cellular migration and apoptosis evasion via modulation of β-catenin protein level |
| title_fullStr | Telomerase RNA component lncRNA as potential diagnostic biomarker promotes CRC cellular migration and apoptosis evasion via modulation of β-catenin protein level |
| title_full_unstemmed | Telomerase RNA component lncRNA as potential diagnostic biomarker promotes CRC cellular migration and apoptosis evasion via modulation of β-catenin protein level |
| title_short | Telomerase RNA component lncRNA as potential diagnostic biomarker promotes CRC cellular migration and apoptosis evasion via modulation of β-catenin protein level |
| title_sort | telomerase rna component lncrna as potential diagnostic biomarker promotes crc cellular migration and apoptosis evasion via modulation of β catenin protein level |
| topic | Colorectal cancer LncRNAs TERC Diagnostic biomarkers Apoptosis evading β-catenin protein |
| url | http://www.sciencedirect.com/science/article/pii/S2468054023000203 |
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