uORF Shuffling Fine-Tunes Gene Expression at a Deep Level of the Process
Upstream open reading frames (uORFs) are present in the 5’ leader sequences (or 5’ untranslated regions) upstream of the protein-coding main ORFs (mORFs) in eukaryotic polycistronic mRNA. It is well known that a uORF negatively affects translation of the mORF. Emerging ribosome profiling approaches...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/9/5/608 |
| _version_ | 1797568284305915904 |
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| author | Yukio Kurihara |
| author_facet | Yukio Kurihara |
| author_sort | Yukio Kurihara |
| collection | DOAJ |
| description | Upstream open reading frames (uORFs) are present in the 5’ leader sequences (or 5’ untranslated regions) upstream of the protein-coding main ORFs (mORFs) in eukaryotic polycistronic mRNA. It is well known that a uORF negatively affects translation of the mORF. Emerging ribosome profiling approaches have revealed that uORFs themselves, as well as downstream mORFs, can be translated. However, it has also been revealed that plants can fine-tune gene expression by modulating uORF-mediated regulation in some situations. This article reviews several proposed mechanisms that enable genes to escape from uORF-mediated negative regulation and gives insight into the application of uORF-mediated regulation for precisely controlling gene expression. |
| first_indexed | 2024-03-10T19:55:16Z |
| format | Article |
| id | doaj.art-329a207945e74aeb95a81a623100505c |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T19:55:16Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-329a207945e74aeb95a81a623100505c2023-11-20T00:00:29ZengMDPI AGPlants2223-77472020-05-019560810.3390/plants9050608uORF Shuffling Fine-Tunes Gene Expression at a Deep Level of the ProcessYukio Kurihara0Synthetic Genomics Research Group, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehirocho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, JapanUpstream open reading frames (uORFs) are present in the 5’ leader sequences (or 5’ untranslated regions) upstream of the protein-coding main ORFs (mORFs) in eukaryotic polycistronic mRNA. It is well known that a uORF negatively affects translation of the mORF. Emerging ribosome profiling approaches have revealed that uORFs themselves, as well as downstream mORFs, can be translated. However, it has also been revealed that plants can fine-tune gene expression by modulating uORF-mediated regulation in some situations. This article reviews several proposed mechanisms that enable genes to escape from uORF-mediated negative regulation and gives insight into the application of uORF-mediated regulation for precisely controlling gene expression.https://www.mdpi.com/2223-7747/9/5/608uORFtranslationribosomeNMD |
| spellingShingle | Yukio Kurihara uORF Shuffling Fine-Tunes Gene Expression at a Deep Level of the Process Plants uORF translation ribosome NMD |
| title | uORF Shuffling Fine-Tunes Gene Expression at a Deep Level of the Process |
| title_full | uORF Shuffling Fine-Tunes Gene Expression at a Deep Level of the Process |
| title_fullStr | uORF Shuffling Fine-Tunes Gene Expression at a Deep Level of the Process |
| title_full_unstemmed | uORF Shuffling Fine-Tunes Gene Expression at a Deep Level of the Process |
| title_short | uORF Shuffling Fine-Tunes Gene Expression at a Deep Level of the Process |
| title_sort | uorf shuffling fine tunes gene expression at a deep level of the process |
| topic | uORF translation ribosome NMD |
| url | https://www.mdpi.com/2223-7747/9/5/608 |
| work_keys_str_mv | AT yukiokurihara uorfshufflingfinetunesgeneexpressionatadeepleveloftheprocess |