Compartmentalized Self-Replication under fast PCR cycling conditions yields Taq DNA polymerase mutants with increased DNA-binding affinity and blood resistance

Compartmentalized Self-Replication under fast PCR cycling conditions yields Taq DNA polymerase mutants with increased DNA-binding affinity and blood resistance

Faster-cycling PCR formulations, protocols, and instruments have been developed to address the need for increased throughput and shorter turn-around times for PCR-based assays. Although run times can be cut by up to 50%, shorter cycle times have been correlated with lower detection sensitivity and i...

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Bibliographic Details
Main Authors: Bahram eArezi, Nancy eMckinney, Connie eHansen, Michelle eCayouette, Jeffrey eFox, Keith eChen, Jennifer eLapira, Sarah eHamilton, Holly eHogrefe
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-08-01
Series:Frontiers in Microbiology
Subjects:
fast PCR
fast cycling
Taq mutants
blood resistant
inhibitor resistant
inhibitor resistant Taq
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00408/full

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