Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation
In this study, we investigated preparation of gradient chitosan-matrix hydrogels through a novel freezing–gelling–thawing method. The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/14/4888 |
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| author | Karolina Kosowska Patrycja Domalik-Pyzik Małgorzata Sekuła-Stryjewska Sylwia Noga Joanna Jagiełło Magdalena Baran Ludwika Lipińska Ewa Zuba-Surma Jan Chłopek |
| author_facet | Karolina Kosowska Patrycja Domalik-Pyzik Małgorzata Sekuła-Stryjewska Sylwia Noga Joanna Jagiełło Magdalena Baran Ludwika Lipińska Ewa Zuba-Surma Jan Chłopek |
| author_sort | Karolina Kosowska |
| collection | DOAJ |
| description | In this study, we investigated preparation of gradient chitosan-matrix hydrogels through a novel freezing–gelling–thawing method. The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds. The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton’s jelly mesenchymal stem cells (hUC-MSCs). We followed the green-chemistry approach and avoided toxic cross-linking agents, using instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives. It was shown that the most promising are the gradient hydrogels modified with GO-PEG and HAp. |
| first_indexed | 2024-03-10T18:32:23Z |
| format | Article |
| id | doaj.art-329d0127fdd94e06b07fb8d04275b36a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T18:32:23Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-329d0127fdd94e06b07fb8d04275b36a2023-11-20T06:28:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-07-012114488810.3390/ijms21144888Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility EvaluationKarolina Kosowska0Patrycja Domalik-Pyzik1Małgorzata Sekuła-Stryjewska2Sylwia Noga3Joanna Jagiełło4Magdalena Baran5Ludwika Lipińska6Ewa Zuba-Surma7Jan Chłopek8Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, 30-059 Krakow, PolandDepartment of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, 30-059 Krakow, PolandMalopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, PolandMalopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, PolandDepartment of Chemical Synthesis and Flake Graphene, Łukasiewicz Research Network—Institute of Electronic Materials Technology, 01-919 Warsaw, PolandDepartment of Chemical Synthesis and Flake Graphene, Łukasiewicz Research Network—Institute of Electronic Materials Technology, 01-919 Warsaw, PolandDepartment of Chemical Synthesis and Flake Graphene, Łukasiewicz Research Network—Institute of Electronic Materials Technology, 01-919 Warsaw, PolandDepartment of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, PolandDepartment of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, 30-059 Krakow, PolandIn this study, we investigated preparation of gradient chitosan-matrix hydrogels through a novel freezing–gelling–thawing method. The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds. The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton’s jelly mesenchymal stem cells (hUC-MSCs). We followed the green-chemistry approach and avoided toxic cross-linking agents, using instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives. It was shown that the most promising are the gradient hydrogels modified with GO-PEG and HAp.https://www.mdpi.com/1422-0067/21/14/4888chitosangraphene family materialshydrogelsgradient nanocomposites |
| spellingShingle | Karolina Kosowska Patrycja Domalik-Pyzik Małgorzata Sekuła-Stryjewska Sylwia Noga Joanna Jagiełło Magdalena Baran Ludwika Lipińska Ewa Zuba-Surma Jan Chłopek Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation International Journal of Molecular Sciences chitosan graphene family materials hydrogels gradient nanocomposites |
| title | Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation |
| title_full | Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation |
| title_fullStr | Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation |
| title_full_unstemmed | Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation |
| title_short | Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation |
| title_sort | gradient chitosan hydrogels modified with graphene derivatives and hydroxyapatite physiochemical properties and initial cytocompatibility evaluation |
| topic | chitosan graphene family materials hydrogels gradient nanocomposites |
| url | https://www.mdpi.com/1422-0067/21/14/4888 |
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