In Vitro Cultures of <i>Scutellaria brevibracteata</i> subsp. <i>subvelutina</i> as a Source of Bioactive Phenolic Metabolites
Some of the more than 350 <i>Scutellaria</i> species, such as <i>S. baicalensis</i> and <i>S. lateriflora</i>, have been used in traditional medicine and today play an important role in official phytotherapy. Other species have been less investigated, and their th...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/28/4/1785 |
| _version_ | 1797619004042379264 |
|---|---|
| author | Inga Kwiecień Aleksandra Łukaszyk Natalizia Miceli Maria Fernanda Taviano Federica Davì Elżbieta Kędzia Halina Ekiert |
| author_facet | Inga Kwiecień Aleksandra Łukaszyk Natalizia Miceli Maria Fernanda Taviano Federica Davì Elżbieta Kędzia Halina Ekiert |
| author_sort | Inga Kwiecień |
| collection | DOAJ |
| description | Some of the more than 350 <i>Scutellaria</i> species, such as <i>S. baicalensis</i> and <i>S. lateriflora</i>, have been used in traditional medicine and today play an important role in official phytotherapy. Other species have been less investigated, and their therapeutic potential is unknown. This is one of the few studies on <i>Scutellaria brevibracteata</i> subsp. <i>subvelutina</i>, and the first research of this species’ in vitro cultures. The aim of this study was to establish an in vitro culture and analyse its phytochemical profile and biological activity. In the methanolic extracts from biomass cultured on six solid Murashige and Skoog (MS) medium variants supplemented with different combinations of 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) in the range 0.5–3 mg/L analysed by HPLC, the presence of specific flavonoids (baicalein, baicalin, wogonin, wogonoside, scutellarin, chrysin), phenylpropanoid glycosides (verbascoside, isoverbascoside), and phenolic acids (<i>p</i>-hydroxybenzoic, caffeic, ferulic, <i>m</i>-coumaric acids) was confirmed. The dominant metabolites were wogonoside and verbascoside with the highest content of 346 and 457 mg/100 g DW, respectively. Thus, the extract with the highest content of bioactive metabolites was selected for further research and subjected to evaluation of antioxidant and antimicrobial potential. The extract exhibited good free radical scavenging activity (IC<sub>50</sub> = 0.92 ± 0.01 mg/mL) and moderate reducing power and chelating activity. The brine shrimp lethality bioassay proved its lack of biotoxicity. Antimicrobial activity was tested against sixteen strains of Gram-positive and Gram-negative bacteria and fungi. The strongest growth inhibitory activity was observed against <i>Trichophyton tonsurans</i>. |
| first_indexed | 2024-03-11T08:20:49Z |
| format | Article |
| id | doaj.art-329ea917c8f14af0822cf091050578c4 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T08:20:49Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-329ea917c8f14af0822cf091050578c42023-11-16T22:23:12ZengMDPI AGMolecules1420-30492023-02-01284178510.3390/molecules28041785In Vitro Cultures of <i>Scutellaria brevibracteata</i> subsp. <i>subvelutina</i> as a Source of Bioactive Phenolic MetabolitesInga Kwiecień0Aleksandra Łukaszyk1Natalizia Miceli2Maria Fernanda Taviano3Federica Davì4Elżbieta Kędzia5Halina Ekiert6Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna St., 30-688 Kraków, PolandDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna St., 30-688 Kraków, PolandDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d’Alcontres, 31, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d’Alcontres, 31, 98166 Messina, ItalyFoundation “Prof. Antonio Imbesi”, University of Messina, Piazza Pugliatti 1, 98122 Messina, ItalyDepartment of Bioproducts Engineering, Institute of Natural Fibres and Medicinal Plants, National Research Institute, 71B Wojska Polskiego St., 60-630 Poznań, PolandDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna St., 30-688 Kraków, PolandSome of the more than 350 <i>Scutellaria</i> species, such as <i>S. baicalensis</i> and <i>S. lateriflora</i>, have been used in traditional medicine and today play an important role in official phytotherapy. Other species have been less investigated, and their therapeutic potential is unknown. This is one of the few studies on <i>Scutellaria brevibracteata</i> subsp. <i>subvelutina</i>, and the first research of this species’ in vitro cultures. The aim of this study was to establish an in vitro culture and analyse its phytochemical profile and biological activity. In the methanolic extracts from biomass cultured on six solid Murashige and Skoog (MS) medium variants supplemented with different combinations of 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) in the range 0.5–3 mg/L analysed by HPLC, the presence of specific flavonoids (baicalein, baicalin, wogonin, wogonoside, scutellarin, chrysin), phenylpropanoid glycosides (verbascoside, isoverbascoside), and phenolic acids (<i>p</i>-hydroxybenzoic, caffeic, ferulic, <i>m</i>-coumaric acids) was confirmed. The dominant metabolites were wogonoside and verbascoside with the highest content of 346 and 457 mg/100 g DW, respectively. Thus, the extract with the highest content of bioactive metabolites was selected for further research and subjected to evaluation of antioxidant and antimicrobial potential. The extract exhibited good free radical scavenging activity (IC<sub>50</sub> = 0.92 ± 0.01 mg/mL) and moderate reducing power and chelating activity. The brine shrimp lethality bioassay proved its lack of biotoxicity. Antimicrobial activity was tested against sixteen strains of Gram-positive and Gram-negative bacteria and fungi. The strongest growth inhibitory activity was observed against <i>Trichophyton tonsurans</i>.https://www.mdpi.com/1420-3049/28/4/1785<i>Scutellaria brevibracteata</i> subsp. <i>subvelutina</i>in vitro culturePGRs testingantioxidant activityantibacterial propertiesantifungal activity |
| spellingShingle | Inga Kwiecień Aleksandra Łukaszyk Natalizia Miceli Maria Fernanda Taviano Federica Davì Elżbieta Kędzia Halina Ekiert In Vitro Cultures of <i>Scutellaria brevibracteata</i> subsp. <i>subvelutina</i> as a Source of Bioactive Phenolic Metabolites Molecules <i>Scutellaria brevibracteata</i> subsp. <i>subvelutina</i> in vitro culture PGRs testing antioxidant activity antibacterial properties antifungal activity |
| title | In Vitro Cultures of <i>Scutellaria brevibracteata</i> subsp. <i>subvelutina</i> as a Source of Bioactive Phenolic Metabolites |
| title_full | In Vitro Cultures of <i>Scutellaria brevibracteata</i> subsp. <i>subvelutina</i> as a Source of Bioactive Phenolic Metabolites |
| title_fullStr | In Vitro Cultures of <i>Scutellaria brevibracteata</i> subsp. <i>subvelutina</i> as a Source of Bioactive Phenolic Metabolites |
| title_full_unstemmed | In Vitro Cultures of <i>Scutellaria brevibracteata</i> subsp. <i>subvelutina</i> as a Source of Bioactive Phenolic Metabolites |
| title_short | In Vitro Cultures of <i>Scutellaria brevibracteata</i> subsp. <i>subvelutina</i> as a Source of Bioactive Phenolic Metabolites |
| title_sort | in vitro cultures of i scutellaria brevibracteata i subsp i subvelutina i as a source of bioactive phenolic metabolites |
| topic | <i>Scutellaria brevibracteata</i> subsp. <i>subvelutina</i> in vitro culture PGRs testing antioxidant activity antibacterial properties antifungal activity |
| url | https://www.mdpi.com/1420-3049/28/4/1785 |
| work_keys_str_mv | AT ingakwiecien invitroculturesofiscutellariabrevibracteataisubspisubvelutinaiasasourceofbioactivephenolicmetabolites AT aleksandrałukaszyk invitroculturesofiscutellariabrevibracteataisubspisubvelutinaiasasourceofbioactivephenolicmetabolites AT nataliziamiceli invitroculturesofiscutellariabrevibracteataisubspisubvelutinaiasasourceofbioactivephenolicmetabolites AT mariafernandataviano invitroculturesofiscutellariabrevibracteataisubspisubvelutinaiasasourceofbioactivephenolicmetabolites AT federicadavi invitroculturesofiscutellariabrevibracteataisubspisubvelutinaiasasourceofbioactivephenolicmetabolites AT elzbietakedzia invitroculturesofiscutellariabrevibracteataisubspisubvelutinaiasasourceofbioactivephenolicmetabolites AT halinaekiert invitroculturesofiscutellariabrevibracteataisubspisubvelutinaiasasourceofbioactivephenolicmetabolites |