Metabolite profiling during graft union formation reveals the reprogramming of primary metabolism and the induction of stilbene synthesis at the graft interface in grapevine
Abstract Background Grafting with rootstocks is essential for the culture of many perennial fruit crops and is increasing being used in the production of annual fruits and vegetables. Our previous work based on microarrays showed that transcripts encoding enzymes of both primary and secondary metabo...
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| Format: | Article |
| Language: | English |
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BMC
2019-12-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-019-2055-9 |
| _version_ | 1818953024281772032 |
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| author | Duyên Prodhomme Josep Valls Fonayet Cyril Hévin Céline Franc Ghislaine Hilbert Gilles de Revel Tristan Richard Nathalie Ollat Sarah Jane Cookson |
| author_facet | Duyên Prodhomme Josep Valls Fonayet Cyril Hévin Céline Franc Ghislaine Hilbert Gilles de Revel Tristan Richard Nathalie Ollat Sarah Jane Cookson |
| author_sort | Duyên Prodhomme |
| collection | DOAJ |
| description | Abstract Background Grafting with rootstocks is essential for the culture of many perennial fruit crops and is increasing being used in the production of annual fruits and vegetables. Our previous work based on microarrays showed that transcripts encoding enzymes of both primary and secondary metabolism were differentially expressed during graft union formation in both homo-grafts (a genotype grafted with itself) and hetero-grafts (two different genotypes grafted together). The aim of this study was to profile primary and secondary metabolites, and quantify the activity of phenylalanine ammonia lyase (PAL) and neutral invertase (NI) in the scion and rootstock tissues and the graft interface of homo and hetero-grafts of grapevine 1 month after grafting. Table-top grafting was done on over-wintering stems (canes) of grapevine and the graft interface tissues (containing some woody stem tissues and callus) were compared to the surrounding rootstock and scion tissues. The objective was to identify compounds involved in graft union formation and hetero-grafting responses. Results A total of 54 compounds from primary and secondary metabolism (19 amino acids, five primary and 30 secondary compounds metabolites) and the activity of two enzymes were measured. The graft interface was associated with an increase in the accumulation of the branched-chain amino acids, basic amino acids, certain stilbene compounds and higher PAL and NI activity in comparison to the surrounding woody stem tissues. Some amino acids and stilbenes were identified as being accumulated differently between the graft interfaces of the scion/rootstock combinations in a manner which was unrelated to their concentrations in the surrounding woody stem tissues. Conclusions This study revealed the modification of primary metabolism to support callus cell formation and the stimulation of stilbene synthesis at the graft interface, and how these processes are modified by hetero-grafting. Knowledge of the metabolites and/or enzymes required for successful graft union formation offer us the potential to identify markers that could be used by nurseries and researchers for selection and breeding purposes. |
| first_indexed | 2024-12-20T09:59:41Z |
| format | Article |
| id | doaj.art-329f7b46ec70484993bdde8b1fd00eb2 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-20T09:59:41Z |
| publishDate | 2019-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-329f7b46ec70484993bdde8b1fd00eb22022-12-21T19:44:21ZengBMCBMC Plant Biology1471-22292019-12-0119111210.1186/s12870-019-2055-9Metabolite profiling during graft union formation reveals the reprogramming of primary metabolism and the induction of stilbene synthesis at the graft interface in grapevineDuyên Prodhomme0Josep Valls Fonayet1Cyril Hévin2Céline Franc3Ghislaine Hilbert4Gilles de Revel5Tristan Richard6Nathalie Ollat7Sarah Jane Cookson8INRA, Univ. Bordeaux, ISVV, EGFV UMR 1287Unité de recherche Oenologie, EA 4577, USC 1366 INRA, ISVV, Université de BordeauxINRA, Univ. Bordeaux, ISVV, EGFV UMR 1287Unité de recherche Oenologie, EA 4577, USC 1366 INRA, ISVV, Université de BordeauxINRA, Univ. Bordeaux, ISVV, EGFV UMR 1287Unité de recherche Oenologie, EA 4577, USC 1366 INRA, ISVV, Université de BordeauxUnité de recherche Oenologie, EA 4577, USC 1366 INRA, ISVV, Université de BordeauxINRA, Univ. Bordeaux, ISVV, EGFV UMR 1287INRA, Univ. Bordeaux, ISVV, EGFV UMR 1287Abstract Background Grafting with rootstocks is essential for the culture of many perennial fruit crops and is increasing being used in the production of annual fruits and vegetables. Our previous work based on microarrays showed that transcripts encoding enzymes of both primary and secondary metabolism were differentially expressed during graft union formation in both homo-grafts (a genotype grafted with itself) and hetero-grafts (two different genotypes grafted together). The aim of this study was to profile primary and secondary metabolites, and quantify the activity of phenylalanine ammonia lyase (PAL) and neutral invertase (NI) in the scion and rootstock tissues and the graft interface of homo and hetero-grafts of grapevine 1 month after grafting. Table-top grafting was done on over-wintering stems (canes) of grapevine and the graft interface tissues (containing some woody stem tissues and callus) were compared to the surrounding rootstock and scion tissues. The objective was to identify compounds involved in graft union formation and hetero-grafting responses. Results A total of 54 compounds from primary and secondary metabolism (19 amino acids, five primary and 30 secondary compounds metabolites) and the activity of two enzymes were measured. The graft interface was associated with an increase in the accumulation of the branched-chain amino acids, basic amino acids, certain stilbene compounds and higher PAL and NI activity in comparison to the surrounding woody stem tissues. Some amino acids and stilbenes were identified as being accumulated differently between the graft interfaces of the scion/rootstock combinations in a manner which was unrelated to their concentrations in the surrounding woody stem tissues. Conclusions This study revealed the modification of primary metabolism to support callus cell formation and the stimulation of stilbene synthesis at the graft interface, and how these processes are modified by hetero-grafting. Knowledge of the metabolites and/or enzymes required for successful graft union formation offer us the potential to identify markers that could be used by nurseries and researchers for selection and breeding purposes.https://doi.org/10.1186/s12870-019-2055-9GraftingGrapevineScionRootstockCanesWood |
| spellingShingle | Duyên Prodhomme Josep Valls Fonayet Cyril Hévin Céline Franc Ghislaine Hilbert Gilles de Revel Tristan Richard Nathalie Ollat Sarah Jane Cookson Metabolite profiling during graft union formation reveals the reprogramming of primary metabolism and the induction of stilbene synthesis at the graft interface in grapevine BMC Plant Biology Grafting Grapevine Scion Rootstock Canes Wood |
| title | Metabolite profiling during graft union formation reveals the reprogramming of primary metabolism and the induction of stilbene synthesis at the graft interface in grapevine |
| title_full | Metabolite profiling during graft union formation reveals the reprogramming of primary metabolism and the induction of stilbene synthesis at the graft interface in grapevine |
| title_fullStr | Metabolite profiling during graft union formation reveals the reprogramming of primary metabolism and the induction of stilbene synthesis at the graft interface in grapevine |
| title_full_unstemmed | Metabolite profiling during graft union formation reveals the reprogramming of primary metabolism and the induction of stilbene synthesis at the graft interface in grapevine |
| title_short | Metabolite profiling during graft union formation reveals the reprogramming of primary metabolism and the induction of stilbene synthesis at the graft interface in grapevine |
| title_sort | metabolite profiling during graft union formation reveals the reprogramming of primary metabolism and the induction of stilbene synthesis at the graft interface in grapevine |
| topic | Grafting Grapevine Scion Rootstock Canes Wood |
| url | https://doi.org/10.1186/s12870-019-2055-9 |
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