Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomass
Abstract Second-generation bioethanol production using lignocellulosic biomass as feedstock requires a highly efficient multistress-tolerant yeast. This study aimed to develop a robust yeast strain of P. kudriavzevii via the adaptive laboratory evolution (ALE) technique. The parental strain of P. ku...
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| Format: | Article |
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-48408-7 |
| _version_ | 1797415312703881216 |
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| author | Sureeporn Dolpatcha Huynh Xuan Phong Sudarat Thanonkeo Preekamol Klanrit Mamoru Yamada Pornthap Thanonkeo |
| author_facet | Sureeporn Dolpatcha Huynh Xuan Phong Sudarat Thanonkeo Preekamol Klanrit Mamoru Yamada Pornthap Thanonkeo |
| author_sort | Sureeporn Dolpatcha |
| collection | DOAJ |
| description | Abstract Second-generation bioethanol production using lignocellulosic biomass as feedstock requires a highly efficient multistress-tolerant yeast. This study aimed to develop a robust yeast strain of P. kudriavzevii via the adaptive laboratory evolution (ALE) technique. The parental strain of P. kudriavzevii was subjected to repetitive long-term cultivation in medium supplemented with a gradually increasing concentration of acetic acid, the major weak acid liberated during the lignocellulosic pretreatment process. Three evolved P. kudriavzevii strains, namely, PkAC-7, PkAC-8, and PkAC-9, obtained in this study exhibited significantly higher resistance toward multiple stressors, including heat, ethanol, osmotic stress, acetic acid, formic acid, furfural, 5-(hydroxymethyl) furfural (5-HMF), and vanillin. The fermentation efficiency of the evolved strains was also improved, yielding a higher ethanol concentration, productivity, and yield than the parental strain, using undetoxified sugarcane bagasse hydrolysate as feedstock. These findings provide evidence that ALE is a practical approach for increasing the multistress tolerance of P. kudriavzevii for stable and efficient second-generation bioethanol production from lignocellulosic biomass. |
| first_indexed | 2024-03-09T05:46:54Z |
| format | Article |
| id | doaj.art-32a07a0d17ee492bac1e29b5e0d0aec9 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T05:46:54Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-32a07a0d17ee492bac1e29b5e0d0aec92023-12-03T12:20:24ZengNature PortfolioScientific Reports2045-23222023-11-0113111510.1038/s41598-023-48408-7Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomassSureeporn Dolpatcha0Huynh Xuan Phong1Sudarat Thanonkeo2Preekamol Klanrit3Mamoru Yamada4Pornthap Thanonkeo5Department of Biotechnology, Faculty of Technology, Khon Kaen UniversityDepartment of Microbial Biotechnology, Institute of Food and Biotechnology, Can Tho UniversityWalai Rukhavej Botanical Research Institute, Mahasarakham UniversityDepartment of Biotechnology, Faculty of Technology, Khon Kaen UniversityDepartment of Biological Chemistry, Faculty of Agriculture, Yamaguchi UniversityDepartment of Biotechnology, Faculty of Technology, Khon Kaen UniversityAbstract Second-generation bioethanol production using lignocellulosic biomass as feedstock requires a highly efficient multistress-tolerant yeast. This study aimed to develop a robust yeast strain of P. kudriavzevii via the adaptive laboratory evolution (ALE) technique. The parental strain of P. kudriavzevii was subjected to repetitive long-term cultivation in medium supplemented with a gradually increasing concentration of acetic acid, the major weak acid liberated during the lignocellulosic pretreatment process. Three evolved P. kudriavzevii strains, namely, PkAC-7, PkAC-8, and PkAC-9, obtained in this study exhibited significantly higher resistance toward multiple stressors, including heat, ethanol, osmotic stress, acetic acid, formic acid, furfural, 5-(hydroxymethyl) furfural (5-HMF), and vanillin. The fermentation efficiency of the evolved strains was also improved, yielding a higher ethanol concentration, productivity, and yield than the parental strain, using undetoxified sugarcane bagasse hydrolysate as feedstock. These findings provide evidence that ALE is a practical approach for increasing the multistress tolerance of P. kudriavzevii for stable and efficient second-generation bioethanol production from lignocellulosic biomass.https://doi.org/10.1038/s41598-023-48408-7 |
| spellingShingle | Sureeporn Dolpatcha Huynh Xuan Phong Sudarat Thanonkeo Preekamol Klanrit Mamoru Yamada Pornthap Thanonkeo Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomass Scientific Reports |
| title | Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomass |
| title_full | Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomass |
| title_fullStr | Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomass |
| title_full_unstemmed | Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomass |
| title_short | Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomass |
| title_sort | adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of pichia kudriavzevii from lignocellulosic biomass |
| url | https://doi.org/10.1038/s41598-023-48408-7 |
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