Mesenchymal Stem Cell Differentiation Driven by Osteoinductive Bioactive Nanoscale Topographies
Human mesenchymal stem cells (hMSCs) respond to the characteristics of their surrounding microenvironment, i.e., their extracellular matrix (ECM). The possibility of mimicking the ECM offers the opportunity to elicit specific cell behaviors. The control of surface properties of a biomaterial at the...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/23/11209 |
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| author | Catarina R. Pedrosa Christel Chanseau Christine Labrugère Sivashankar Krishnamoorthy Marie-Christine Durrieu |
| author_facet | Catarina R. Pedrosa Christel Chanseau Christine Labrugère Sivashankar Krishnamoorthy Marie-Christine Durrieu |
| author_sort | Catarina R. Pedrosa |
| collection | DOAJ |
| description | Human mesenchymal stem cells (hMSCs) respond to the characteristics of their surrounding microenvironment, i.e., their extracellular matrix (ECM). The possibility of mimicking the ECM offers the opportunity to elicit specific cell behaviors. The control of surface properties of a biomaterial at the scale level of the components of the ECM has the potential to effectively modulate cell response. Ordered nanoscale silicon pillar arrays were fabricated using reverse micelles of block copolymers on full wafers, with standard deviations lower than 15%. Bioactive synthetic peptides were covalently grafted on nanoarrays to evaluate possible synergies between chemistry and topography on osteogenic differentiation of hMSCs. Functionalization with RGD (Arg-Gly-Asp) and BMP-2 (bone morphogenetic protein-2) mimetic peptides lead to an enhancement of osteogenic differentiation. Bare nanopillar arrays of reduced pitch were found to promote faster hMSC differentiation. These findings highlight the relevance of investigating possibilities of engineering in vitro systems which can be fine-tuned according to the envisaged cell response. |
| first_indexed | 2024-03-10T04:57:00Z |
| format | Article |
| id | doaj.art-32a2560b2ca64be29eba3efae9fe9dc4 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T04:57:00Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-32a2560b2ca64be29eba3efae9fe9dc42023-11-23T02:04:20ZengMDPI AGApplied Sciences2076-34172021-11-0111231120910.3390/app112311209Mesenchymal Stem Cell Differentiation Driven by Osteoinductive Bioactive Nanoscale TopographiesCatarina R. Pedrosa0Christel Chanseau1Christine Labrugère2Sivashankar Krishnamoorthy3Marie-Christine Durrieu4Chimie et Biologie des Membranes et Nano-Objets (UMR5248 CBMN), Université de Bordeaux, Allée Geoffroy Saint Hilaire-Bât 14, 33600 Pessac, FranceChimie et Biologie des Membranes et Nano-Objets (UMR5248 CBMN), Université de Bordeaux, Allée Geoffroy Saint Hilaire-Bât 14, 33600 Pessac, FrancePLACAMAT, UMS3626, CNRS-Université de Bordeaux, 87 Avenue Docteur Albert Schweitzer, 33600 Pessac, FranceMaterials Research and Technology (MRT) Department, Luxembourg Institute of Science and Technology (LIST), 41 Rue du Brill, Belvaux, Luxembourg L-4422, LuxembourgChimie et Biologie des Membranes et Nano-Objets (UMR5248 CBMN), Université de Bordeaux, Allée Geoffroy Saint Hilaire-Bât 14, 33600 Pessac, FranceHuman mesenchymal stem cells (hMSCs) respond to the characteristics of their surrounding microenvironment, i.e., their extracellular matrix (ECM). The possibility of mimicking the ECM offers the opportunity to elicit specific cell behaviors. The control of surface properties of a biomaterial at the scale level of the components of the ECM has the potential to effectively modulate cell response. Ordered nanoscale silicon pillar arrays were fabricated using reverse micelles of block copolymers on full wafers, with standard deviations lower than 15%. Bioactive synthetic peptides were covalently grafted on nanoarrays to evaluate possible synergies between chemistry and topography on osteogenic differentiation of hMSCs. Functionalization with RGD (Arg-Gly-Asp) and BMP-2 (bone morphogenetic protein-2) mimetic peptides lead to an enhancement of osteogenic differentiation. Bare nanopillar arrays of reduced pitch were found to promote faster hMSC differentiation. These findings highlight the relevance of investigating possibilities of engineering in vitro systems which can be fine-tuned according to the envisaged cell response.https://www.mdpi.com/2076-3417/11/23/11209nanotopographysurface functionalizationmimetic peptidemesenchymal stem cellosteogenic differentiation |
| spellingShingle | Catarina R. Pedrosa Christel Chanseau Christine Labrugère Sivashankar Krishnamoorthy Marie-Christine Durrieu Mesenchymal Stem Cell Differentiation Driven by Osteoinductive Bioactive Nanoscale Topographies Applied Sciences nanotopography surface functionalization mimetic peptide mesenchymal stem cell osteogenic differentiation |
| title | Mesenchymal Stem Cell Differentiation Driven by Osteoinductive Bioactive Nanoscale Topographies |
| title_full | Mesenchymal Stem Cell Differentiation Driven by Osteoinductive Bioactive Nanoscale Topographies |
| title_fullStr | Mesenchymal Stem Cell Differentiation Driven by Osteoinductive Bioactive Nanoscale Topographies |
| title_full_unstemmed | Mesenchymal Stem Cell Differentiation Driven by Osteoinductive Bioactive Nanoscale Topographies |
| title_short | Mesenchymal Stem Cell Differentiation Driven by Osteoinductive Bioactive Nanoscale Topographies |
| title_sort | mesenchymal stem cell differentiation driven by osteoinductive bioactive nanoscale topographies |
| topic | nanotopography surface functionalization mimetic peptide mesenchymal stem cell osteogenic differentiation |
| url | https://www.mdpi.com/2076-3417/11/23/11209 |
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