Impact of Isoorientin on Metabolic Activity and Lipid Accumulation in Differentiated Adipocytes
The current study explored the effect of isoorientin on the metabolic activity and lipid accumulation in fully differentiated 3T3-L1 adipocytes. To achieve this, the 3T3-L1 pre-adipocytes were differentiated for eight days and treated with various concentrations of isoorientin (0.1–100 μM) for four...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/25/8/1773 |
| _version_ | 1797570816824573952 |
|---|---|
| author | Khanyisani Ziqubu Christo J. F. Muller Phiwayinkosi V. Dludla Sinenhlanhla X. H. Mthembu Nnini Obonye Johan Louw Abidemi P. Kappo Sonia Silvestri Patrick Orlando Luca Tiano Sithandiwe E. Mazibuko-Mbeje |
| author_facet | Khanyisani Ziqubu Christo J. F. Muller Phiwayinkosi V. Dludla Sinenhlanhla X. H. Mthembu Nnini Obonye Johan Louw Abidemi P. Kappo Sonia Silvestri Patrick Orlando Luca Tiano Sithandiwe E. Mazibuko-Mbeje |
| author_sort | Khanyisani Ziqubu |
| collection | DOAJ |
| description | The current study explored the effect of isoorientin on the metabolic activity and lipid accumulation in fully differentiated 3T3-L1 adipocytes. To achieve this, the 3T3-L1 pre-adipocytes were differentiated for eight days and treated with various concentrations of isoorientin (0.1–100 μM) for four hours. Subsequently, the metabolic activity, lipid accumulation, and mitochondrial respiration were assessed. Furthermore, to unravel the molecular mechanisms that might elucidate the bioactivity of isoorientin, protein expression of the genes involved in insulin signaling and energy expenditure, such as AKT and AMPK, were investigated. The results showed that isoorientin, at different doses, could block lipid storage and enhance glycerol release, with a concomitant improvement of the metabolic activity and mitochondrial function. Although the observed beneficial effects of isoorientin on these cultured 3T3-L1 adipocytes were not consistent at all concentrations, it was clear that doses between 1 and 10 μM were most effective compared to the untreated control. Moreover, the activity of isoorientin was comparable to tested positive controls of CL-316,2431, isoproterenol, insulin, and metformin. Mechanistically, protein expression of AKT and AMPK, was enhanced with isoorientin exposure, suggesting their partial role in modulating lipid metabolism and mitochondrial biogenesis. Indeed, our results showed that isoorientin has the ability to enhance mitochondrial respiration, as we observed an increase in the ATP and oxygen consumption rate. Therefore, we concluded that isoorientin has a potential to impact mitochondrial activity, lipid metabolism and energy expenditure using an in vitro experimental model of obesity. |
| first_indexed | 2024-03-10T20:30:46Z |
| format | Article |
| id | doaj.art-32a3642d8a194dc5bf4f66f19f041cdc |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T20:30:46Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-32a3642d8a194dc5bf4f66f19f041cdc2023-11-19T21:26:22ZengMDPI AGMolecules1420-30492020-04-01258177310.3390/molecules25081773Impact of Isoorientin on Metabolic Activity and Lipid Accumulation in Differentiated AdipocytesKhanyisani Ziqubu0Christo J. F. Muller1Phiwayinkosi V. Dludla2Sinenhlanhla X. H. Mthembu3Nnini Obonye4Johan Louw5Abidemi P. Kappo6Sonia Silvestri7Patrick Orlando8Luca Tiano9Sithandiwe E. Mazibuko-Mbeje10Biomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg 7505, South AfricaBiomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg 7505, South AfricaBiomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg 7505, South AfricaBiomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg 7505, South AfricaBiomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg 7505, South AfricaBiomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg 7505, South AfricaDepartment of Biochemistry and Microbiology, University of Zululand, KwaDlangezwa 3886, South AfricaDepartment of Life and Environmental Sciences, Polytechnic University of Marche, 60131 Ancona, ItalyDepartment of Life and Environmental Sciences, Polytechnic University of Marche, 60131 Ancona, ItalyDepartment of Life and Environmental Sciences, Polytechnic University of Marche, 60131 Ancona, ItalyBiomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg 7505, South AfricaThe current study explored the effect of isoorientin on the metabolic activity and lipid accumulation in fully differentiated 3T3-L1 adipocytes. To achieve this, the 3T3-L1 pre-adipocytes were differentiated for eight days and treated with various concentrations of isoorientin (0.1–100 μM) for four hours. Subsequently, the metabolic activity, lipid accumulation, and mitochondrial respiration were assessed. Furthermore, to unravel the molecular mechanisms that might elucidate the bioactivity of isoorientin, protein expression of the genes involved in insulin signaling and energy expenditure, such as AKT and AMPK, were investigated. The results showed that isoorientin, at different doses, could block lipid storage and enhance glycerol release, with a concomitant improvement of the metabolic activity and mitochondrial function. Although the observed beneficial effects of isoorientin on these cultured 3T3-L1 adipocytes were not consistent at all concentrations, it was clear that doses between 1 and 10 μM were most effective compared to the untreated control. Moreover, the activity of isoorientin was comparable to tested positive controls of CL-316,2431, isoproterenol, insulin, and metformin. Mechanistically, protein expression of AKT and AMPK, was enhanced with isoorientin exposure, suggesting their partial role in modulating lipid metabolism and mitochondrial biogenesis. Indeed, our results showed that isoorientin has the ability to enhance mitochondrial respiration, as we observed an increase in the ATP and oxygen consumption rate. Therefore, we concluded that isoorientin has a potential to impact mitochondrial activity, lipid metabolism and energy expenditure using an in vitro experimental model of obesity.https://www.mdpi.com/1420-3049/25/8/1773isoorientinobesitymetabolic activitylipid accumulation3T3-L1 adipocytes |
| spellingShingle | Khanyisani Ziqubu Christo J. F. Muller Phiwayinkosi V. Dludla Sinenhlanhla X. H. Mthembu Nnini Obonye Johan Louw Abidemi P. Kappo Sonia Silvestri Patrick Orlando Luca Tiano Sithandiwe E. Mazibuko-Mbeje Impact of Isoorientin on Metabolic Activity and Lipid Accumulation in Differentiated Adipocytes Molecules isoorientin obesity metabolic activity lipid accumulation 3T3-L1 adipocytes |
| title | Impact of Isoorientin on Metabolic Activity and Lipid Accumulation in Differentiated Adipocytes |
| title_full | Impact of Isoorientin on Metabolic Activity and Lipid Accumulation in Differentiated Adipocytes |
| title_fullStr | Impact of Isoorientin on Metabolic Activity and Lipid Accumulation in Differentiated Adipocytes |
| title_full_unstemmed | Impact of Isoorientin on Metabolic Activity and Lipid Accumulation in Differentiated Adipocytes |
| title_short | Impact of Isoorientin on Metabolic Activity and Lipid Accumulation in Differentiated Adipocytes |
| title_sort | impact of isoorientin on metabolic activity and lipid accumulation in differentiated adipocytes |
| topic | isoorientin obesity metabolic activity lipid accumulation 3T3-L1 adipocytes |
| url | https://www.mdpi.com/1420-3049/25/8/1773 |
| work_keys_str_mv | AT khanyisaniziqubu impactofisoorientinonmetabolicactivityandlipidaccumulationindifferentiatedadipocytes AT christojfmuller impactofisoorientinonmetabolicactivityandlipidaccumulationindifferentiatedadipocytes AT phiwayinkosivdludla impactofisoorientinonmetabolicactivityandlipidaccumulationindifferentiatedadipocytes AT sinenhlanhlaxhmthembu impactofisoorientinonmetabolicactivityandlipidaccumulationindifferentiatedadipocytes AT nniniobonye impactofisoorientinonmetabolicactivityandlipidaccumulationindifferentiatedadipocytes AT johanlouw impactofisoorientinonmetabolicactivityandlipidaccumulationindifferentiatedadipocytes AT abidemipkappo impactofisoorientinonmetabolicactivityandlipidaccumulationindifferentiatedadipocytes AT soniasilvestri impactofisoorientinonmetabolicactivityandlipidaccumulationindifferentiatedadipocytes AT patrickorlando impactofisoorientinonmetabolicactivityandlipidaccumulationindifferentiatedadipocytes AT lucatiano impactofisoorientinonmetabolicactivityandlipidaccumulationindifferentiatedadipocytes AT sithandiweemazibukombeje impactofisoorientinonmetabolicactivityandlipidaccumulationindifferentiatedadipocytes |