Fabrication, Characterization, and Antimicrobial Activity of Carvacrol-Loaded Zein Nanoparticles Using the pH-Driven Method
To reduce the application of synthetic additives in the field of food preservation, this study utilized carvacrol as an antibacterial agent, and zein and sodium caseinate as carriers, to prepare composite nanoparticles loaded with carvacrol by the pH-driven method. The composite nanoparticles of zei...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/16/9227 |
| _version_ | 1797409732208623616 |
|---|---|
| author | Huaming Zheng Jiangli Wang Feng You Mingyu Zhou Shengwei Shi |
| author_facet | Huaming Zheng Jiangli Wang Feng You Mingyu Zhou Shengwei Shi |
| author_sort | Huaming Zheng |
| collection | DOAJ |
| description | To reduce the application of synthetic additives in the field of food preservation, this study utilized carvacrol as an antibacterial agent, and zein and sodium caseinate as carriers, to prepare composite nanoparticles loaded with carvacrol by the pH-driven method. The composite nanoparticles of zein/sodium caseinate had an excellent encapsulation efficiency (77.96~82.19%) for carvacrol, and it had remarkable redispersibility. The results of Fourier transform infrared spectroscopy showed that the formation of the composite nanoparticles mainly depended on the hydrogen bond and the hydrophobic zone force, and thermal gravimetric analysis showed that carvacrol was loaded successfully into nanoparticles, and loading efficiency reached 24.9%. Scanning electron microscopy showed that the composite nanoparticles were spherical, with a particle size range of 50~200 nm, and through the free radical scavenging method and the plate counting method to confirm the particle has stronger antioxidant and antibacterial properties, and with the composite nanoparticles with poly (vinyl alcohol) film applied to the preservation of banana together, it was found that PVA film containing 5 wt% CA-loaded composite NPs can significantly extend the storage period of banana. Therefore, when the composite nanoparticles were applied to food packaging, they could effectively inhibit food spoilage and lengthen the shelf life of food, which displays potential application prospects in the food industry. |
| first_indexed | 2024-03-09T04:19:37Z |
| format | Article |
| id | doaj.art-32a3a0c724124b10a18418a1200a7c08 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:19:37Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-32a3a0c724124b10a18418a1200a7c082023-12-03T13:49:14ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316922710.3390/ijms23169227Fabrication, Characterization, and Antimicrobial Activity of Carvacrol-Loaded Zein Nanoparticles Using the pH-Driven MethodHuaming Zheng0Jiangli Wang1Feng You2Mingyu Zhou3Shengwei Shi4Hubei Key Lab of Plasma Chemistry and Advanced Materials, Wuhan Institute of Technology, Wuhan 430205, ChinaHubei Key Lab of Plasma Chemistry and Advanced Materials, Wuhan Institute of Technology, Wuhan 430205, ChinaHubei Key Lab of Plasma Chemistry and Advanced Materials, Wuhan Institute of Technology, Wuhan 430205, ChinaHubei Key Lab of Plasma Chemistry and Advanced Materials, Wuhan Institute of Technology, Wuhan 430205, ChinaHubei Key Lab of Plasma Chemistry and Advanced Materials, Wuhan Institute of Technology, Wuhan 430205, ChinaTo reduce the application of synthetic additives in the field of food preservation, this study utilized carvacrol as an antibacterial agent, and zein and sodium caseinate as carriers, to prepare composite nanoparticles loaded with carvacrol by the pH-driven method. The composite nanoparticles of zein/sodium caseinate had an excellent encapsulation efficiency (77.96~82.19%) for carvacrol, and it had remarkable redispersibility. The results of Fourier transform infrared spectroscopy showed that the formation of the composite nanoparticles mainly depended on the hydrogen bond and the hydrophobic zone force, and thermal gravimetric analysis showed that carvacrol was loaded successfully into nanoparticles, and loading efficiency reached 24.9%. Scanning electron microscopy showed that the composite nanoparticles were spherical, with a particle size range of 50~200 nm, and through the free radical scavenging method and the plate counting method to confirm the particle has stronger antioxidant and antibacterial properties, and with the composite nanoparticles with poly (vinyl alcohol) film applied to the preservation of banana together, it was found that PVA film containing 5 wt% CA-loaded composite NPs can significantly extend the storage period of banana. Therefore, when the composite nanoparticles were applied to food packaging, they could effectively inhibit food spoilage and lengthen the shelf life of food, which displays potential application prospects in the food industry.https://www.mdpi.com/1422-0067/23/16/9227zeincarvacrolcomposite nanoparticlessodium caseinatepH-drivenantimicrobial activity |
| spellingShingle | Huaming Zheng Jiangli Wang Feng You Mingyu Zhou Shengwei Shi Fabrication, Characterization, and Antimicrobial Activity of Carvacrol-Loaded Zein Nanoparticles Using the pH-Driven Method International Journal of Molecular Sciences zein carvacrol composite nanoparticles sodium caseinate pH-driven antimicrobial activity |
| title | Fabrication, Characterization, and Antimicrobial Activity of Carvacrol-Loaded Zein Nanoparticles Using the pH-Driven Method |
| title_full | Fabrication, Characterization, and Antimicrobial Activity of Carvacrol-Loaded Zein Nanoparticles Using the pH-Driven Method |
| title_fullStr | Fabrication, Characterization, and Antimicrobial Activity of Carvacrol-Loaded Zein Nanoparticles Using the pH-Driven Method |
| title_full_unstemmed | Fabrication, Characterization, and Antimicrobial Activity of Carvacrol-Loaded Zein Nanoparticles Using the pH-Driven Method |
| title_short | Fabrication, Characterization, and Antimicrobial Activity of Carvacrol-Loaded Zein Nanoparticles Using the pH-Driven Method |
| title_sort | fabrication characterization and antimicrobial activity of carvacrol loaded zein nanoparticles using the ph driven method |
| topic | zein carvacrol composite nanoparticles sodium caseinate pH-driven antimicrobial activity |
| url | https://www.mdpi.com/1422-0067/23/16/9227 |
| work_keys_str_mv | AT huamingzheng fabricationcharacterizationandantimicrobialactivityofcarvacrolloadedzeinnanoparticlesusingthephdrivenmethod AT jiangliwang fabricationcharacterizationandantimicrobialactivityofcarvacrolloadedzeinnanoparticlesusingthephdrivenmethod AT fengyou fabricationcharacterizationandantimicrobialactivityofcarvacrolloadedzeinnanoparticlesusingthephdrivenmethod AT mingyuzhou fabricationcharacterizationandantimicrobialactivityofcarvacrolloadedzeinnanoparticlesusingthephdrivenmethod AT shengweishi fabricationcharacterizationandantimicrobialactivityofcarvacrolloadedzeinnanoparticlesusingthephdrivenmethod |