A Renewable Biosensor Based on LNA-Aptamer Hybridization for the Detection of <i>Salmonella enteritidis</i> in <i>Penaeus vannamei</i>
<i>Salmonella enteritidis</i> (SE) is an important factor causing foodborne disease, and electrochemical sensors have drawn much attention for SE prevention and detection due to their many advantages. A renewable electrochemical sensor using specially designed locked nucleic acids (LNA)...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/1/450 |
| _version_ | 1797406626421932032 |
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| author | Zhihua Li Ling Zhao Qian Wu Xue Zhang Xiaowei Huang Jiyong Shi Xiaobo Zou |
| author_facet | Zhihua Li Ling Zhao Qian Wu Xue Zhang Xiaowei Huang Jiyong Shi Xiaobo Zou |
| author_sort | Zhihua Li |
| collection | DOAJ |
| description | <i>Salmonella enteritidis</i> (SE) is an important factor causing foodborne disease, and electrochemical sensors have drawn much attention for SE prevention and detection due to their many advantages. A renewable electrochemical sensor using specially designed locked nucleic acids (LNA) as linkers for the detection of SE was proposed to improve the reusability and reproducibility of biosensors. One end of the LNA was designed as an anchor to attach to modified electrodes through the sulfhydryl group; the other end was used to match with a short segment of SE aptamers, which will allow for the convenient renewal of occupied aptamers by raising the temperature. Results revealed that the manufactured biosensor had good stability, reproducibility, and selectivity in addition to a linear range of 6 × 10<sup>1</sup>–6 × 10<sup>5</sup> CFU/mL and a limit of detection (LOD) of 20.704 CFU/mL. The recovery rate of SE for the real sample varied from 98.84% to 134.82% without exceeding 16.27% in the relative standard deviation (RSD). The proposed biosensor appears to be a promising tool for foodborne pathogen detection. |
| first_indexed | 2024-03-09T03:29:17Z |
| format | Article |
| id | doaj.art-32a62a4e10164069a5f7700d090a1462 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T03:29:17Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-32a62a4e10164069a5f7700d090a14622023-12-03T14:58:05ZengMDPI AGMolecules1420-30492023-01-0128145010.3390/molecules28010450A Renewable Biosensor Based on LNA-Aptamer Hybridization for the Detection of <i>Salmonella enteritidis</i> in <i>Penaeus vannamei</i>Zhihua Li0Ling Zhao1Qian Wu2Xue Zhang3Xiaowei Huang4Jiyong Shi5Xiaobo Zou6School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China<i>Salmonella enteritidis</i> (SE) is an important factor causing foodborne disease, and electrochemical sensors have drawn much attention for SE prevention and detection due to their many advantages. A renewable electrochemical sensor using specially designed locked nucleic acids (LNA) as linkers for the detection of SE was proposed to improve the reusability and reproducibility of biosensors. One end of the LNA was designed as an anchor to attach to modified electrodes through the sulfhydryl group; the other end was used to match with a short segment of SE aptamers, which will allow for the convenient renewal of occupied aptamers by raising the temperature. Results revealed that the manufactured biosensor had good stability, reproducibility, and selectivity in addition to a linear range of 6 × 10<sup>1</sup>–6 × 10<sup>5</sup> CFU/mL and a limit of detection (LOD) of 20.704 CFU/mL. The recovery rate of SE for the real sample varied from 98.84% to 134.82% without exceeding 16.27% in the relative standard deviation (RSD). The proposed biosensor appears to be a promising tool for foodborne pathogen detection.https://www.mdpi.com/1420-3049/28/1/450locked nucleic acidsaptamer<i>Salmonella enteritidis</i>renewable electrochemical sensor |
| spellingShingle | Zhihua Li Ling Zhao Qian Wu Xue Zhang Xiaowei Huang Jiyong Shi Xiaobo Zou A Renewable Biosensor Based on LNA-Aptamer Hybridization for the Detection of <i>Salmonella enteritidis</i> in <i>Penaeus vannamei</i> Molecules locked nucleic acids aptamer <i>Salmonella enteritidis</i> renewable electrochemical sensor |
| title | A Renewable Biosensor Based on LNA-Aptamer Hybridization for the Detection of <i>Salmonella enteritidis</i> in <i>Penaeus vannamei</i> |
| title_full | A Renewable Biosensor Based on LNA-Aptamer Hybridization for the Detection of <i>Salmonella enteritidis</i> in <i>Penaeus vannamei</i> |
| title_fullStr | A Renewable Biosensor Based on LNA-Aptamer Hybridization for the Detection of <i>Salmonella enteritidis</i> in <i>Penaeus vannamei</i> |
| title_full_unstemmed | A Renewable Biosensor Based on LNA-Aptamer Hybridization for the Detection of <i>Salmonella enteritidis</i> in <i>Penaeus vannamei</i> |
| title_short | A Renewable Biosensor Based on LNA-Aptamer Hybridization for the Detection of <i>Salmonella enteritidis</i> in <i>Penaeus vannamei</i> |
| title_sort | renewable biosensor based on lna aptamer hybridization for the detection of i salmonella enteritidis i in i penaeus vannamei i |
| topic | locked nucleic acids aptamer <i>Salmonella enteritidis</i> renewable electrochemical sensor |
| url | https://www.mdpi.com/1420-3049/28/1/450 |
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