Transcriptomic Response of <i>Saccharomyces cerevisiae</i> during Fermentation under Oleic Acid and Ergosterol Depletion
Under anaerobic/hypoxic conditions, <i>Saccharomyces cerevisiae</i> relies on external lipid supplements to modulate membrane lipid fraction in response to different stresses. Here, transcriptomic responses of two <i>S. cerevisiae</i> wine strains were evaluated during hypoxi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/5/3/57 |
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| author | Giacomo Zara Hennie J. J. van Vuuren Ilaria Mannazzu Severino Zara Marilena Budroni |
| author_facet | Giacomo Zara Hennie J. J. van Vuuren Ilaria Mannazzu Severino Zara Marilena Budroni |
| author_sort | Giacomo Zara |
| collection | DOAJ |
| description | Under anaerobic/hypoxic conditions, <i>Saccharomyces cerevisiae</i> relies on external lipid supplements to modulate membrane lipid fraction in response to different stresses. Here, transcriptomic responses of two <i>S. cerevisiae</i> wine strains were evaluated during hypoxic fermentation of a synthetic must with/without ergosterol and oleic acid supplementation. In the absence of lipids, the two strains, namely EC1118 and M25, showed different behaviour, with M25 significantly decreasing its fermentation rate from the 72 h after inoculum. At this time point, the whole genome transcriptomic analysis revealed common and strain-specific responses to the lack of lipid supplementation. Common responses included the upregulation of the genes involved in ergosterol biosynthesis, as well as the seripauperin and the heat shock protein multigene families. In addition, the upregulation of the aerobic isoforms of genes involved in mitochondrial electron transport is compatible with the previously observed accumulation of reactive oxygen species in the two strains during growth in absence of lipids. Considering the strain-specific responses, M25 downregulated the transcription of genes involved in glucose transport, methionine biosynthesis and of those encoding mannoproteins required for adaptation to low temperatures and hypoxia. The identification of these pathways, which are presumably involved in yeast resistance to stresses, will assist industrial strain selection. |
| first_indexed | 2024-12-12T01:58:57Z |
| format | Article |
| id | doaj.art-32a6b62506004a09a0767c19e46892f2 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-12-12T01:58:57Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-32a6b62506004a09a0767c19e46892f22022-12-22T00:42:15ZengMDPI AGFermentation2311-56372019-07-01535710.3390/fermentation5030057fermentation5030057Transcriptomic Response of <i>Saccharomyces cerevisiae</i> during Fermentation under Oleic Acid and Ergosterol DepletionGiacomo Zara0Hennie J. J. van Vuuren1Ilaria Mannazzu2Severino Zara3Marilena Budroni4Department of Agriculture, University of Sassari, 07100 Sassari, ItalyWine Research Centre, The University of British Columbia, Vancouver, BC V6T 1Z4, CanadaDepartment of Agriculture, University of Sassari, 07100 Sassari, ItalyDepartment of Agriculture, University of Sassari, 07100 Sassari, ItalyDepartment of Agriculture, University of Sassari, 07100 Sassari, ItalyUnder anaerobic/hypoxic conditions, <i>Saccharomyces cerevisiae</i> relies on external lipid supplements to modulate membrane lipid fraction in response to different stresses. Here, transcriptomic responses of two <i>S. cerevisiae</i> wine strains were evaluated during hypoxic fermentation of a synthetic must with/without ergosterol and oleic acid supplementation. In the absence of lipids, the two strains, namely EC1118 and M25, showed different behaviour, with M25 significantly decreasing its fermentation rate from the 72 h after inoculum. At this time point, the whole genome transcriptomic analysis revealed common and strain-specific responses to the lack of lipid supplementation. Common responses included the upregulation of the genes involved in ergosterol biosynthesis, as well as the seripauperin and the heat shock protein multigene families. In addition, the upregulation of the aerobic isoforms of genes involved in mitochondrial electron transport is compatible with the previously observed accumulation of reactive oxygen species in the two strains during growth in absence of lipids. Considering the strain-specific responses, M25 downregulated the transcription of genes involved in glucose transport, methionine biosynthesis and of those encoding mannoproteins required for adaptation to low temperatures and hypoxia. The identification of these pathways, which are presumably involved in yeast resistance to stresses, will assist industrial strain selection.https://www.mdpi.com/2311-5637/5/3/57wine fermentationergosteroloxidative stress<i>TIR/TIP</i>methionine biosynthesis |
| spellingShingle | Giacomo Zara Hennie J. J. van Vuuren Ilaria Mannazzu Severino Zara Marilena Budroni Transcriptomic Response of <i>Saccharomyces cerevisiae</i> during Fermentation under Oleic Acid and Ergosterol Depletion Fermentation wine fermentation ergosterol oxidative stress <i>TIR/TIP</i> methionine biosynthesis |
| title | Transcriptomic Response of <i>Saccharomyces cerevisiae</i> during Fermentation under Oleic Acid and Ergosterol Depletion |
| title_full | Transcriptomic Response of <i>Saccharomyces cerevisiae</i> during Fermentation under Oleic Acid and Ergosterol Depletion |
| title_fullStr | Transcriptomic Response of <i>Saccharomyces cerevisiae</i> during Fermentation under Oleic Acid and Ergosterol Depletion |
| title_full_unstemmed | Transcriptomic Response of <i>Saccharomyces cerevisiae</i> during Fermentation under Oleic Acid and Ergosterol Depletion |
| title_short | Transcriptomic Response of <i>Saccharomyces cerevisiae</i> during Fermentation under Oleic Acid and Ergosterol Depletion |
| title_sort | transcriptomic response of i saccharomyces cerevisiae i during fermentation under oleic acid and ergosterol depletion |
| topic | wine fermentation ergosterol oxidative stress <i>TIR/TIP</i> methionine biosynthesis |
| url | https://www.mdpi.com/2311-5637/5/3/57 |
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