Characterization of pulmonary intimal sarcoma cells isolated from a surgical specimen: In vitro and in vivo study.
Pulmonary intimal sarcoma (PIS) constitutes a rare sarcoma originating from the intimal cells of pulmonary arteries. The pathogenesis of PIS remains to be elucidated and specific treatments have not been established; therefore, prognosis is generally poor. The purpose of our study was to isolate and...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0214654 |
| _version_ | 1819144206037286912 |
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| author | Takayuki Jujo Sanada Seiichiro Sakao Akira Naito Hatsue Ishibashi-Ueda Masaki Suga Hiroki Shoji Hideki Miwa Rika Suda Shunichiro Iwasawa Yuji Tada Keiichi Ishida Nobuhiro Tanabe Koichiro Tatsumi |
| author_facet | Takayuki Jujo Sanada Seiichiro Sakao Akira Naito Hatsue Ishibashi-Ueda Masaki Suga Hiroki Shoji Hideki Miwa Rika Suda Shunichiro Iwasawa Yuji Tada Keiichi Ishida Nobuhiro Tanabe Koichiro Tatsumi |
| author_sort | Takayuki Jujo Sanada |
| collection | DOAJ |
| description | Pulmonary intimal sarcoma (PIS) constitutes a rare sarcoma originating from the intimal cells of pulmonary arteries. The pathogenesis of PIS remains to be elucidated and specific treatments have not been established; therefore, prognosis is generally poor. The purpose of our study was to isolate and characterize PIS cells from a specimen resected from a patient with PIS. The surgical specimen was minced and incubated, and spindle-shaped and small cells were successfully isolated and designated as PIS-1. PIS-1 cells at passages 8-9 were used for all in vitro and in vivo experiments. Immunocytochemistry showed that PIS-1 cells were positive for vimentin, murine double minute 2, and CD44 and negative for α-smooth muscle actin, CD31, von Willebrand factor, and desmin. PIS-1 cells exhibited the hallmarks of malignant cells including the potential for autonomous proliferation, anchorage-independent growth, invasion, genetic instability, and tumorigenicity in severe combined immunodeficiency mice. The PIS-1 cells highly expressed tyrosine kinase receptors such as platelet-derived growth factor receptor, and vascular endothelial growth factor receptor 2. Pazopanib, a multi-targeted tyrosine kinase inhibitor, suppressed the proliferation of PIS-1 cells in vitro and the growth of tumors formed from xenografted PIS-1 cells. A PIS cell line was thus successfully established. The PIS-1 cells highly expressed tyrosine kinase receptors, which may be a target for treatment of PIS. |
| first_indexed | 2024-12-22T12:38:27Z |
| format | Article |
| id | doaj.art-32a6caa925cf46e08590fa6cbd6ff1a3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T12:38:27Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-32a6caa925cf46e08590fa6cbd6ff1a32022-12-21T18:25:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01143e021465410.1371/journal.pone.0214654Characterization of pulmonary intimal sarcoma cells isolated from a surgical specimen: In vitro and in vivo study.Takayuki Jujo SanadaSeiichiro SakaoAkira NaitoHatsue Ishibashi-UedaMasaki SugaHiroki ShojiHideki MiwaRika SudaShunichiro IwasawaYuji TadaKeiichi IshidaNobuhiro TanabeKoichiro TatsumiPulmonary intimal sarcoma (PIS) constitutes a rare sarcoma originating from the intimal cells of pulmonary arteries. The pathogenesis of PIS remains to be elucidated and specific treatments have not been established; therefore, prognosis is generally poor. The purpose of our study was to isolate and characterize PIS cells from a specimen resected from a patient with PIS. The surgical specimen was minced and incubated, and spindle-shaped and small cells were successfully isolated and designated as PIS-1. PIS-1 cells at passages 8-9 were used for all in vitro and in vivo experiments. Immunocytochemistry showed that PIS-1 cells were positive for vimentin, murine double minute 2, and CD44 and negative for α-smooth muscle actin, CD31, von Willebrand factor, and desmin. PIS-1 cells exhibited the hallmarks of malignant cells including the potential for autonomous proliferation, anchorage-independent growth, invasion, genetic instability, and tumorigenicity in severe combined immunodeficiency mice. The PIS-1 cells highly expressed tyrosine kinase receptors such as platelet-derived growth factor receptor, and vascular endothelial growth factor receptor 2. Pazopanib, a multi-targeted tyrosine kinase inhibitor, suppressed the proliferation of PIS-1 cells in vitro and the growth of tumors formed from xenografted PIS-1 cells. A PIS cell line was thus successfully established. The PIS-1 cells highly expressed tyrosine kinase receptors, which may be a target for treatment of PIS.https://doi.org/10.1371/journal.pone.0214654 |
| spellingShingle | Takayuki Jujo Sanada Seiichiro Sakao Akira Naito Hatsue Ishibashi-Ueda Masaki Suga Hiroki Shoji Hideki Miwa Rika Suda Shunichiro Iwasawa Yuji Tada Keiichi Ishida Nobuhiro Tanabe Koichiro Tatsumi Characterization of pulmonary intimal sarcoma cells isolated from a surgical specimen: In vitro and in vivo study. PLoS ONE |
| title | Characterization of pulmonary intimal sarcoma cells isolated from a surgical specimen: In vitro and in vivo study. |
| title_full | Characterization of pulmonary intimal sarcoma cells isolated from a surgical specimen: In vitro and in vivo study. |
| title_fullStr | Characterization of pulmonary intimal sarcoma cells isolated from a surgical specimen: In vitro and in vivo study. |
| title_full_unstemmed | Characterization of pulmonary intimal sarcoma cells isolated from a surgical specimen: In vitro and in vivo study. |
| title_short | Characterization of pulmonary intimal sarcoma cells isolated from a surgical specimen: In vitro and in vivo study. |
| title_sort | characterization of pulmonary intimal sarcoma cells isolated from a surgical specimen in vitro and in vivo study |
| url | https://doi.org/10.1371/journal.pone.0214654 |
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