Time-Restricted Feeding Could Not Reduce Rainbow Trout Lipid Deposition Induced by Artificial Night Light
Artificial night light (ALAN) could lead to circadian rhythm disorders and disrupt normal lipid metabolism, while time-restricted feeding (TRF) could maintain metabolic homeostasis. In mammals, TRF has been demonstrated to have extraordinary effects on the metabolic regulation caused by circadian rh...
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MDPI AG
2022-09-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/10/904 |
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| author | Hanying Xu Ce Shi Yangfang Ye Changbin Song Changkao Mu Chunlin Wang |
| author_facet | Hanying Xu Ce Shi Yangfang Ye Changbin Song Changkao Mu Chunlin Wang |
| author_sort | Hanying Xu |
| collection | DOAJ |
| description | Artificial night light (ALAN) could lead to circadian rhythm disorders and disrupt normal lipid metabolism, while time-restricted feeding (TRF) could maintain metabolic homeostasis. In mammals, TRF has been demonstrated to have extraordinary effects on the metabolic regulation caused by circadian rhythm disorders, but studies in lower vertebrates such as fish are still scarce. In this study, the impacts of ALAN on the body composition and lipid metabolism of juvenile rainbow trout were investigated by continuous light (LL) exposure as well as whether TRF could alleviate the negative effects of LL. The results showed that LL upregulated the expression of lipid synthesis (<i>fas</i> and <i>srebp-1c</i>) genes and suppressed the expression of lipid lipolysis (<i>pparβ</i>, <i>cpt-1a</i>, and <i>lpl</i>) genes in the liver, finally promoting lipid accumulation in juvenile rainbow trout. However, LL downregulated the expression of genes (<i>Δ6-fad</i>, <i>Δ9-fad</i>, <i>elovl2</i>, and <i>elovl5</i>) related to long-chain polyunsaturated fatty acid (LC-PUFA) synthesis, resulting in a significant decrease in the proportion of LC-PUFA in the dorsal muscle. In serum, LL led to a decrease in glucose (Glu) levels and an increase in triglyceride (TG) and high-density lipoprotein cholesterol (H-DLC) levels. On the other hand, TRF (mid-dark stage feeding (D)) and mid-light stage feeding (L)) upregulated the expression of both the lipid synthesis (<i>srebp-1c</i> and <i>pparγ</i>), lipolysis (<i>pparα</i>, <i>pparβ</i>, and <i>cpt-1a</i>), and lipid transport (<i>cd36</i>/<i>fat</i> and <i>fatp-1</i>) genes, finally increasing the whole-body lipid, liver protein, and lipid content. Meanwhile, TRF (D and L groups) increased the proportion of polyunsaturated fatty acid (PUFA) and LC-PUFA in serum. In contrast, random feeding (R group) increased the serum Glu levels and decreased TG, total cholesterol (T-CHO), and H-DLC levels, suggesting stress and poor nutritional status. In conclusion, ALAN led to lipid accumulation and a significant decrease in muscle LC-PUFA proportion, and TRF failed to rescue these negative effects. |
| first_indexed | 2024-03-09T19:49:02Z |
| format | Article |
| id | doaj.art-32a73a658be94aacbcd0b43ff299a85e |
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| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T19:49:02Z |
| publishDate | 2022-09-01 |
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| spelling | doaj.art-32a73a658be94aacbcd0b43ff299a85e2023-11-24T01:14:53ZengMDPI AGMetabolites2218-19892022-09-01121090410.3390/metabo12100904Time-Restricted Feeding Could Not Reduce Rainbow Trout Lipid Deposition Induced by Artificial Night LightHanying Xu0Ce Shi1Yangfang Ye2Changbin Song3Changkao Mu4Chunlin Wang5Key Laboratory of Aquacultural Biotechnology, Ministry of Education, Ningbo University, 818 Fenghua Road, Ningbo 315211, ChinaKey Laboratory of Aquacultural Biotechnology, Ministry of Education, Ningbo University, 818 Fenghua Road, Ningbo 315211, ChinaKey Laboratory of Aquacultural Biotechnology, Ministry of Education, Ningbo University, 818 Fenghua Road, Ningbo 315211, ChinaInstitute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, ChinaKey Laboratory of Aquacultural Biotechnology, Ministry of Education, Ningbo University, 818 Fenghua Road, Ningbo 315211, ChinaKey Laboratory of Aquacultural Biotechnology, Ministry of Education, Ningbo University, 818 Fenghua Road, Ningbo 315211, ChinaArtificial night light (ALAN) could lead to circadian rhythm disorders and disrupt normal lipid metabolism, while time-restricted feeding (TRF) could maintain metabolic homeostasis. In mammals, TRF has been demonstrated to have extraordinary effects on the metabolic regulation caused by circadian rhythm disorders, but studies in lower vertebrates such as fish are still scarce. In this study, the impacts of ALAN on the body composition and lipid metabolism of juvenile rainbow trout were investigated by continuous light (LL) exposure as well as whether TRF could alleviate the negative effects of LL. The results showed that LL upregulated the expression of lipid synthesis (<i>fas</i> and <i>srebp-1c</i>) genes and suppressed the expression of lipid lipolysis (<i>pparβ</i>, <i>cpt-1a</i>, and <i>lpl</i>) genes in the liver, finally promoting lipid accumulation in juvenile rainbow trout. However, LL downregulated the expression of genes (<i>Δ6-fad</i>, <i>Δ9-fad</i>, <i>elovl2</i>, and <i>elovl5</i>) related to long-chain polyunsaturated fatty acid (LC-PUFA) synthesis, resulting in a significant decrease in the proportion of LC-PUFA in the dorsal muscle. In serum, LL led to a decrease in glucose (Glu) levels and an increase in triglyceride (TG) and high-density lipoprotein cholesterol (H-DLC) levels. On the other hand, TRF (mid-dark stage feeding (D)) and mid-light stage feeding (L)) upregulated the expression of both the lipid synthesis (<i>srebp-1c</i> and <i>pparγ</i>), lipolysis (<i>pparα</i>, <i>pparβ</i>, and <i>cpt-1a</i>), and lipid transport (<i>cd36</i>/<i>fat</i> and <i>fatp-1</i>) genes, finally increasing the whole-body lipid, liver protein, and lipid content. Meanwhile, TRF (D and L groups) increased the proportion of polyunsaturated fatty acid (PUFA) and LC-PUFA in serum. In contrast, random feeding (R group) increased the serum Glu levels and decreased TG, total cholesterol (T-CHO), and H-DLC levels, suggesting stress and poor nutritional status. In conclusion, ALAN led to lipid accumulation and a significant decrease in muscle LC-PUFA proportion, and TRF failed to rescue these negative effects.https://www.mdpi.com/2218-1989/12/10/904artificial night lightfeeding regimelipid metabolismserum metabolitesrainbow trout |
| spellingShingle | Hanying Xu Ce Shi Yangfang Ye Changbin Song Changkao Mu Chunlin Wang Time-Restricted Feeding Could Not Reduce Rainbow Trout Lipid Deposition Induced by Artificial Night Light Metabolites artificial night light feeding regime lipid metabolism serum metabolites rainbow trout |
| title | Time-Restricted Feeding Could Not Reduce Rainbow Trout Lipid Deposition Induced by Artificial Night Light |
| title_full | Time-Restricted Feeding Could Not Reduce Rainbow Trout Lipid Deposition Induced by Artificial Night Light |
| title_fullStr | Time-Restricted Feeding Could Not Reduce Rainbow Trout Lipid Deposition Induced by Artificial Night Light |
| title_full_unstemmed | Time-Restricted Feeding Could Not Reduce Rainbow Trout Lipid Deposition Induced by Artificial Night Light |
| title_short | Time-Restricted Feeding Could Not Reduce Rainbow Trout Lipid Deposition Induced by Artificial Night Light |
| title_sort | time restricted feeding could not reduce rainbow trout lipid deposition induced by artificial night light |
| topic | artificial night light feeding regime lipid metabolism serum metabolites rainbow trout |
| url | https://www.mdpi.com/2218-1989/12/10/904 |
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